ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells

Shukla, Ritesh K.; Sharma, Vyom; Pandey, Alok Kumar; Singh, Shashi; Sultana, Sarwat; Dhawan, Alok

doi:10.1016/j.tiv.2010.11.008

Cited by 483 publications

(359 citation statements)

References 53 publications

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“…These results are supported by recent reports that an increase in oxidative stress led to an increase in apoptosis formation and DNA damage, and subsequently a decrease in cell viability (Guichard et al, 2012;Jugan et al, 2011;Kumar et al, 2011). Another recent study demonstrated the correlation between DNA damage and micronucleus formation with ROS in human epidermal cells treated by anatase nano-TiO 2 (Shukla et al, 2011). Nanoparticles compared to their larger counterparts having the same mass weight possess a higher surface area to volume ratio, which enhance contact area with their surroundings and may induce more formation of ROS (Fig.…”

Section: Most Of the Toxicological Results On Nps Have Been Generatedsupporting

confidence: 78%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

119

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/npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr Access and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://dx.doi.org/10. 1016/j.tiv.2012.12.018 Toxicology in Vitro, 27, 2, pp. 864-873, 2012-12-28 In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells Hamzeh, Mahsa; Sunahara, Geoffrey I. Therefore, our objective was to investigate the cytotoxicity and genotoxicity of commercially available TiO 2 nanoparticles with respect to their selected physicochemical properties, as well as the role of surface coating of these nanoparticles. While all types of tested TiO 2 samples decrease cell viability in a mass-based concentration-and size-dependent manner, the polyacrylate-coated nano-TiO 2 product was only cytotoxic at higher concentrations. A similar pattern of response was observed for induction of apoptosis/necrosis, and no DNA damage was detected in the polyacrylate-coated nano-TiO 2 model. Given the increasing production of TiO 2 nanoparticles, toxicological studies should take into account the physiochemical properties of these nanoparticles that may help researchers to develop new nanoparticles with minimum toxicity.

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Section: Most Of the Toxicological Results On Nps Have Been Generatedsupporting

confidence: 78%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

119

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“…It has been demonstrated in literature that carbon nanotubes (CNTs) are capable of inducing reactive oxygen species (ROS) (C. S. Sharma, et al, 2007) and pulmonary effects (Shvedova, et al, 2005). It has also been shown in toxicological studies that nano-sized titanium dioxide (TiO2) particles have the potential to induce cytotoxic (Saquib, et al, 2012;Setyawati, et al, 2012), genotoxic (Shukla, et al, 2011;Trouiller, Reliene, Westbrook, Solaimani, & Schiestl, 2009) and inflammatory effects (Grassian, O'Shaughnessy, Adamcakova-Dodd, Pettibone, & Thorne, 2007;S. G. Han, Newsome, & Hennig, 2013).…”

Section: Nanomaterials Toxicitymentioning

confidence: 99%

(Q)SAR modelling of nanomaterial toxicity: A critical review

et al. 2015

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There is an increasing recognition that nanomaterials pose a risk to human health, and that the novel engineered nanomaterials (ENMs) in the nanotechnology industry and their increasing industrial usage poses the most immediate problem for hazard assessment, as many of them remain untested. The large number of materials and their variants (different sizes and coatings for instance) that require testing and ethical pressure towards non-animal testing means that expensive animal bioassay is precluded, and the use of (quantitative) structure activity relationships ((Q)SAR) models as an alternative source of hazard information should be explored.(Q)SAR modelling can be applied to fill the critical knowledge gaps by making the best use of existing data, prioritize physicochemical parameters driving toxicity, and provide practical solutions to the risk assessment problems caused by the diversity of ENMs. This paper covers the core components required for successful application of (Q)SAR technologies to ENMs toxicity prediction, and summarizes the published nano-(Q)SAR studies and outlines the challenges ahead for nano-(Q)SAR modelling. It provides a critical review of (1) the present status of the availability of ENMs characterization/toxicity data, (2) the characterization of nanostructures that meets the need of (Q)SAR analysis, (3) the summary of published nano-(Q)SAR studies and their limitations, (4) the in silico tools for (Q)SAR screening of nanotoxicity and (5) the prospective directions for the development of nano-(Q)SAR models.

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“…LMA stored at 4°C until required. When needed, briefly melt agarose in microwave and kept on dry/water bath maintained at 37°C (Shukla et al 2011). 2) Normal melting agarose (NMA) -1% NMA is mixed into the distilled water and heat until the agarose dissolves in distilled water.…”

Section: ) Low Melting Agarose (Lma) -1% Lma Is Mixed Intomentioning

confidence: 99%

“…2) Normal melting agarose (NMA) -1% NMA is mixed into the distilled water and heat until the agarose dissolves in distilled water. It always freshly prepared and kept on dry bath at 60°C during the preparation of base slides (Shukla et al 2011). …”

Section: ) Low Melting Agarose (Lma) -1% Lma Is Mixed Intomentioning

confidence: 99%

“…It further disrupts nucleosomes and almost all histones being solubilized by the high salt. Furthermore, it form nucleoids consisting of a nuclear matrix composed of ribonucleic acid (RNA), proteins and containing negatively charged super coiled loops of DNA (Collins 2004;Shukla et al 2011). In this step cover-slips are removed and the slides immersed in a freshly prepared and chilled lysis solution (146.1 g NaCl, 37.2 g EDTA, 1.2 g Tris, pH 10) with 1% Triton X-100 and 10% Dimethyl sulfoxide (DMSO) added just before use.…”

Section: Lysismentioning

confidence: 99%

See 1 more Smart Citation

An introduction to the single cell gel electrophoresis assay: a technique resolving issues in forensic science

Shukla

2018

Egypt J Forensic Sci

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Time since death (TSD) estimation is a crucial issue in death investigation. In majority of homicide/suicide cases deceased body recovered within the first 48 h, therefore, it is critically important to determine the time of death quickly and precisely. In the present scenario, TSD estimation still remains difficult even for experienced pathologists undertaken with extreme caution. After death numerous factors may be of cadaveric or environmental origin can influence the 'normal' rate of postmortem changes. Therefore, it could be more difficult to estimate longer TSD. However, these environmental influencing factors can also effect on DNA in the form of degradation, which start after 1-2 h of death. This DNA degradation could be work as molecular clock which help to estimate early TSD. Single-cell gel electrophoresis (SCGE), also known as the Comet assay, is the technique by which DNA degradation can be visualized and measured qualitatively as well as quantitatively. Using this assay not only early TSD estimated but many other challenges such as time since deposition of biological fluids, repair of genetic material from degraded biological sample could also be resolved. With the help of this paper an attempt was made to introduce a well-known cytogenetic technique that is SCGE assay, which could be a versatile technique for forensic science.

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ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells

Cited by 483 publications

References 53 publications

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

(Q)SAR modelling of nanomaterial toxicity: A critical review

An introduction to the single cell gel electrophoresis assay: a technique resolving issues in forensic science

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