ROS mediated antibacterial activity of photoilluminated riboflavin: A photodynamic mechanism against nosocomial infections

Khan, Shehtaj; P, Mohammed Rayis; Rizvi, Asim; Alam, Mohammad Zubair; Rizvi, Meher; Naseem, Imrana

doi:10.1016/j.toxrep.2019.01.003

Cited by 73 publications

(39 citation statements)

References 40 publications

(41 reference statements)

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“…Recently, interest in silicon carbide films as a promising material for nanoelectronics and Riboflavin (vitamin B2) is an important and necessary drug for the development and viability of the human body. Vitamin preparation is used in the form of powders, and in the form of aqueous solutions [1,2]. Self-assembly is a process in which only the components of the final structure are involved [3,4].…”

Section: Introductionmentioning

confidence: 99%

Hipochromic Effect in Riboflavin Solutions

Astanov¹,

Sharipov²,

Kasimova³

2019

Eurasian phys. tech. j.

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The process of self-assembly of riboflavin molecules in aqueous and binary mixtures of solvents was investigated by a spectroscopic method. It was shown that the self-assembly of vitamin B2 molecules by the dipole-dipole interaction of van der Waals forces, as a result of which resonant splitting of excited electronic levels of riboflavin occurs. In concentrated solutions and in binary mixtures of solvents, the observed hypochromic effects are due to a decrease in the intensity of the absorption capacity of self-aggregated riboflavin molecules relative to their monomers. The absorption band of self-assembled riboflavin molecules is determined by the obtained linear dichroism spectra in a laminar hydrodynamic flow.

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Section: Introductionmentioning

confidence: 99%

Hipochromic Effect in Riboflavin Solutions

Astanov¹,

Sharipov²,

Kasimova³

2019

Eurasian phys. tech. j.

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“…A great deal of literature has confirmed that RF has an excellent antioxidant property that can be taped to nullify the toxic effects of many xenobiotics and drugs [14,27,29,30]. PB-induced toxicities because of its extensive usage in edibles, drinking water, colas, paints, and cosmetics are one of the profound clinical challenges in society.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier, Santos De Souza et al [48] and De Souza Queiroz et al [49] in separate studies on different cell lines reported induction of Fas-FasL-mediated apoptosis by RF under photoillumination. Recently, Khan et al [30] have shown that the photoactivated vitamin caused cell death in mice with lung carcinoma. It is noteworthy that the vitamin is a strong antioxidant in its native form, but it can also act as a prooxidant under photoillumination.…”

mentioning

confidence: 99%

“…It is noteworthy that the vitamin is a strong antioxidant in its native form, but it can also act as a prooxidant under photoillumination. Both of its properties are being exploited for medical intervention in various clinical challenges [30,46]. Besides, its adequate supplementation improves the intestinal mucosa for the absorption of nutrients, enhancing the overall health of the individuals in the many studies [50].…”

mentioning

confidence: 99%

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The Alleviative Effect of Vitamin B₂ on Potassium Bromate-Induced Hepatotoxicity in Male Rats

Hassan

Ebaid

Alhazza

et al. 2020

BioMed Research International

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Potassium bromate (PB) is a food enhancer, water disinfection by-product, and a proven carcinogen. It elicits toxicities in the living organism due to exposure and in a dose-dependent manner. The present study discourses the ameliorative efficacy of riboflavin (RF) in PB-administered rodents. The animals were distributed into five treatment groups: control (group I), PB alone (group II, 150 mg/kg), RF alone (group III, 2 mg/kg), PB+RF1 (group IV, 150 mg/kg+2 mg/kg), and PB+RF2 (group V, 150 mg/kg+4 mg/kg). After the round of the treatment, the animals were sacrificed to collect their blood and liver samples for the detailed analysis. Group II depicted perturbed liver functions evidenced by altered serum and toxicity markers along with the disturbed redox balance. Also, these biochemical results were found harmonious with histopathological analysis and comet assay. However, group III showed no noticeable alteration in the same parameters, whereas the combination groups (IV and V) exhibited dose-dependent amelioration in the PB-induced toxicities. Interestingly, RF favored apoptosis concomitant with suppressing the necrosis in the PB-challenged groups, as shown by the activity of caspase-3 and lactate dehydrogenase. Histopathological analysis and comet assay further consolidate these results. Hence, RF has significant alleviative property against PB-induced hepatotoxicity in vivo that can be used in the consumer items containing the toxicant.

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“…The generation of ROS in PBS was estimated using the 2',7'-dichlorofluorescin diacetate (DCFH-DA) method [29,30]. To achieve the adsorption/desorption equilibrium, polished and nanosecond laser irradiated Zr-BMG samples were immersed in a bacterial solution (0.01 mol/L PBS with 10 7 cfu/mL S. aureus ) for 24 h and then incubated with 500 μL of 2',7'-dichlorofluorescin diacetate (DPBF) solution (10 mM dissolved in DMSO) in the dark for 5 min.…”

Section: Antibacterial Characterization 231 Detection Of Rosmentioning

confidence: 99%

Antibacterial performance of nanosecond laser irradiated zirconium-based bulk metallic glass

Wang

Zhang

et al. 2020

Preprint

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Bulk metallic glass (BMG) have attracted considerable attention during the last decades due to their excellent physical, chemical, and biomedical properties. This report aims to investigate the relationship between antibacterial performance, crystallization behavior and laser ablation parameters of Zr-based bulk metallic glass (Zr-BMG) after nanosecond laser irradiation. The surface morphology, crystallization behavior, surface quality, binding energy and Cu/Ni release properties were explored after laser irradiation. The formation of reactive oxygen species following the immersion of Zr-BMG in phosphate-buffered saline was evaluated using the 2',7'-dichlorofluorescin diacetate method. Staphylococcus aureus was selected to assess the antibacterial performance of Zr-BMG and mouse osteoblasts were used to examine the in vitro cytotoxicity. The results showed that at a laser energy intensity lower than 0.08 J/mm2 the amorphous structure of Zr-BMG was retained after the irradiation process. Furthermore, the laser irradiation process considerably increased the antibacterial performance of Zr-BMG. The Cu/Ni release rate, reactive oxygen species concentration, and antibacterial properties were directly proportional to the laser energy intensity. However, surfaces with a high antibacterial rate also showed high cytotoxicity. The surface irradiated with 7 μJ ablation pulse and 200 mm/s irradiation speed showed a better balance between antibacterial and cytotoxic properties with amorphous status.

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ROS mediated antibacterial activity of photoilluminated riboflavin: A photodynamic mechanism against nosocomial infections

Abstract: HighlightsRiboflavin undergoes intersystem conversion under photoillumination.Interacts with molecular oxygen and generates ROS.Generated ROS disrupts E. coli cell membranes.Ultimately killing E. coli.Mechanism can be used to kill E. coli on hospital ware causing nosocomial infections.

Cited by 73 publications

References 40 publications

Hipochromic Effect in Riboflavin Solutions

Hipochromic Effect in Riboflavin Solutions

The Alleviative Effect of Vitamin B₂ on Potassium Bromate-Induced Hepatotoxicity in Male Rats

Antibacterial performance of nanosecond laser irradiated zirconium-based bulk metallic glass

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ROS mediated antibacterial activity of photoilluminated riboflavin: A photodynamic mechanism against nosocomial infections

Abstract: HighlightsRiboflavin undergoes intersystem conversion under photoillumination.Interacts with molecular oxygen and generates ROS.Generated ROS disrupts E. coli cell membranes.Ultimately killing E. coli.Mechanism can be used to kill E. coli on hospital ware causing nosocomial infections.

Cited by 73 publications

References 40 publications

Hipochromic Effect in Riboflavin Solutions

Hipochromic Effect in Riboflavin Solutions

The Alleviative Effect of Vitamin B2 on Potassium Bromate-Induced Hepatotoxicity in Male Rats

Antibacterial performance of nanosecond laser irradiated zirconium-based bulk metallic glass

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Product

Resources

About

The Alleviative Effect of Vitamin B₂ on Potassium Bromate-Induced Hepatotoxicity in Male Rats