ROS Is a Factor Regulating the Increased Polysaccharide Production by Light Quality in the Edible Cyanobacterium <i>Nostoc flagelliforme</i>

Han, Peipei; Shen, Shi-gang; Guo, Rong-jun; Zhao, Dongxue; Lin, Ya-Hui; Jia, Shiru; Yan, Rong-rong; Wu, Yi-Kai

doi:10.1021/acs.jafc.8b06176

Cited by 40 publications

(31 citation statements)

References 35 publications

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“…Han et al showed in 2018 and 2019, that different light qualities influenced the gene expression of N. flagelliforme, and that cells treated under blue and red light showed more significant changes in carbohydrate metabolism, energy metabolism, amino acid metabolism, nucleotide metabolism, activities of key enzymes and signal transduction. Moreover, light quality was considered to be a key factor controlling the growth and exopolysaccharides biosynthesis (Han et al, 2018;Han et al, 2019).…”

Section: Laboratory Resultsmentioning

confidence: 99%

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Ortiz-Moreno¹,

Cárdenas-Poblador²,

Agredo³

et al. 2020

Univ. Sci.

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Mathematical models provide information about population dynamics under different conditions. In the study, four models were evaluated and employed to describe the growth kinetics of Nostoc ellipsosporum with different light wavelengths: Baranyi-Roberts, Modiﬁed Gompertz, Modiﬁed Logistic, and Richards. N. ellipsosporum was grown in BG-11 liquid medium for 9 days, using 12 hours of photoperiod and the following treatments: white light (400-800 nm), red light (650-800 nm), yellow light (550-580 nm) and blue light (460-480 nm). Each experiment was performed in triplicate. The optical density (OD) was measured on days 1, 3, 5, 7 and 9, using a spectrophotometer at 650 nm. The maximum cell growth was obtained under white light (OD650 : 0.090 ± 0.008), followed by the yellow light (OD650 :0.057 ± 0.004). Conversely, blue light showed a marked inhibitory effect on the growth of N. ellipsosporum (OD650 : 0.009 ± 0.001). The results revealed that the Baranyi-Roberts model had a better ﬁt with the experimental data from N. ellipsosporum growth in all four treatments. The ﬁndings from this modeling study could be used in several biotechnological applications that require the productionof N. ellipsosporum and its bioproducts.

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Section: Laboratory Resultsmentioning

confidence: 99%

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Ortiz-Moreno¹,

Cárdenas-Poblador²,

Agredo³

et al. 2020

Univ. Sci.

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“…Much work has been done on Nostoc flagelliforme [11,61,63,[79][80][81][82][83][84]. This strain is an edible terrestrial cyanobacterium that is used as food in China for more than 2000 years.…”

Section: Process Conditionsmentioning

confidence: 99%

Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

et al. 2020

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Cyanobacteria have the potential to become an industrially sustainable source of functional biopolymers. Their exopolysaccharides (EPS) harbor chemical complexity, which predicts bioactive potential. Although some are reported to excrete conspicuous amounts of polysaccharides, others are still to be discovered. The production of this strain-specific trait can promote carbon neutrality while its intrinsic location can potentially reduce downstream processing costs. To develop an EPS cyanobacterial bioprocess (Cyano-EPS) three steps were explored: the selection of the cyanobacterial host; optimization of production parameters; downstream processing. Studying the production parameters allow us to understand and optimize their response in terms of growth and EPS production though many times it was found divergent. Although the extraction of EPS can be achieved with a certain degree of simplicity, the purification and isolation steps demand experience. In this review, we gathered relevant research on EPS with a focus on bioprocess development. Challenges and strategies to overcome possible drawbacks are highlighted.

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“…Overall, our data suggest that QDs affect the expression levels of a number of proteins by inducing oxidative stress at both treatment times. It is possible to correlate the dysfunction in the glycolysis pathway, the downregulation of oxidative phosphorylation and also the increase in protein misfolding in the ER - all caused by QD treatment-with the production of ROS, which impairs the oxidative balance of the cells and becomes increasingly severe over time [ 44 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs)

et al. 2020

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The use of cadmium sulphide quantum dot (CdS QD)-enabled products has become increasingly widespread. The prospect of their release in the environment is raising concerns. Here we have used the yeast model Saccharomyces cerevisiae to determine the potential impact of CdS QD nanoparticles on living organisms. Proteomic analyses and cell viability assays performed after 9 h exposure revealed expression of proteins involved in oxidative stress and reduced lethality, respectively, whereas oxidative stress declined, and lethality increased after 24 h incubation in the presence of CdS QDs. Quantitative proteomics using the iTRAQ approach (isobaric tags for relative and absolute quantitation) revealed that key proteins involved in essential biological pathways were differentially regulated over the time course of the experiment. At 9 h, most of the glycolytic functions increased, and the abundance of the number of heat shock proteins increased. This contrasts with the situation at 24 h where glycolytic functions, some heat shock proteins as well as oxidative phosphorylation and ATP synthesis were down-regulated. It can be concluded from our data that cell exposure to CdS QDs provokes a metabolic shift from respiration to fermentation, comparable to the situation reported in some cancer cell lines.

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ROS Is a Factor Regulating the Increased Polysaccharide Production by Light Quality in the Edible Cyanobacterium Nostoc flagelliforme

Cited by 40 publications

References 35 publications

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs)

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