ROS-initiated in-situ polymerization of diacetylene-containing lipidated peptide amphiphile in living cells

Lv, Niannian; Ma, Teng; Qin, Huimin; Yang, Zhuoran; Wu, Yanggui; Li, Danqi; Tao, Juan; Jiang, Hao; Zhu, Jintao

doi:10.1007/s40843-022-2008-1

Cited by 9 publications

(4 citation statements)

References 58 publications

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“…According to our previous report, ROS in the intracellular microenvironment could initiate the in situ polymerization reaction of DEVD‐DLPAs. [ 25 ] Therefore, it is expected that the high level of ROS during the second laser irradiation could not only damage mitochondria, trigger mtROS burst, but also promote the in situ polymerization reaction of DEVD‐DLPA. Before investigation of the ROS induced in situ polymerization reaction in living cells, the morphological transformation of DEVD‐DLPA@C3 NPs was investigated.…”

Section: Resultsmentioning

confidence: 99%

Morphological Transformation and In Situ Polymerization of Caspase‐3 Responsive Diacetylene‐Containing Lipidated Peptide Amphiphile for Self‐Amplified Cooperative Antitumor Therapy

Chen

et al. 2022

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In order to artificially regulate cell behaviors, intracellular polymerization as an emerging chemical technique has attracted much attention. Yet, it is still a challenge to achieve effective intracellular polymerization to conquer tumors in the complex cellular environment. Herein, this work develops a tumor‐targeting and caspase‐3 responsive nanoparticle composed of a diacetylene‐containing lipidated peptide amphiphile and mitochondria‐targeting photosensitizer (C3), which undergoes nanoparticle‐to‐nanofiber transformation and efficient in situ polymerization triggered by photodynamic treatment and activation of caspase‐3. The locational nanofibers on the mitochondria membranes lead to mitochondrial reactive oxygen species (mtROS) burst and self‐amplified circulation, offering persistent high oxidative stress to induce cell apoptosis. This study provides a strategy for greatly enhanced antitumor therapeutic efficacy through mtROS burst and self‐amplified circulation induced by intracellular transformation and in situ polymerization.

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Section: Resultsmentioning

confidence: 99%

Morphological Transformation and In Situ Polymerization of Caspase‐3 Responsive Diacetylene‐Containing Lipidated Peptide Amphiphile for Self‐Amplified Cooperative Antitumor Therapy

Chen

et al. 2022

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“…Diacetylene-containing peptide amphiphiles also have the potential of polymerization initiated by intracellular ROS. 165,166 Zhu et al designed two precursors, PA-OH and PA-NH 2 , with different charge properties (Fig. 31).…”

Section: Other Mechanism-mediated Polymerizationmentioning

confidence: 99%

Synthesizing biomaterials in living organisms

Zhang,

Wang,

Zhang

et al. 2023

Chem. Soc. Rev.

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“…Recently, Zhu and coworkers designed lipidated peptides (Y-DLPA) containing diacetylene for ROS-mediated in situ polymerization. 41 The positively charged Y-DLPA1 inside cells were converted to poly(diacetylene) derivatives that showed red-shifted fluorescence for cell tracking. As a demonstration of regulating cell behaviors, the in situ polymerized Y-DLPA1 increased the viscosity and decreased the motility and viability of tumor cells.…”

Section: Redox-responsive Polymerizationmentioning

confidence: 99%

“…In vivo anticancer effects from this ROS-triggered polymerization also implied new access to manipulate cellular activities through designed in situ polymerizations. Recently, Zhu and co-workers designed lipidated peptides (Y-DLPA) containing diacetylene for ROS-mediated in situ polymerization . The positively charged Y-DLPA1 inside cells were converted to poly(diacetylene) derivatives that showed red-shifted fluorescence for cell tracking.…”

Section: In Situ Polymerizations Inside Living Cellsmentioning

confidence: 99%

Polymer Materials Synthesized through Cell-Mediated Polymerization Strategies for Regulation of Biological Functions

et al. 2022

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Metrics & MoreArticle Recommendations CONSPECTUS: As essential components of living organisms, biomacromolecules construct cell scaffolds and regulate cell activities and biological functions through chemical transformations in biological systems. Inspired by the functional evolution in the formation of natural structures, in situ polymerization methods have been developed to create functional synthetic macromolecules inside or on the surface of living cells. Given the diversity of cell species and the complexity of biological pathways, selected strategies can be employed to control the synthesis of functional polymers that utilize the dynamic cellular microenvironment.In this Account, we summarize recent work in the field of designing cell-mediated in situ polymerization methods, with which we demonstrate their application prospects including tumor cell labeling and treatment, microbial photosynthetic efficiency regulation, and hydrogel generation. The purpose of these efforts is to design polymerization reactions in response to endogenous or exogenous stimuli and to describe the underlying response mechanisms. By reasonable design of molecular structures, in situ synthesized polymers in the cell microenvironment implement regulation of biological functions. For example, using specific redox activity combined with light irradiation, bacteria can mediate the generation of functional polymers as the encapsulating matrix or with antibacterial effects. Conjugated polymers synthesized on the microalgae surface expanded the spectral absorption and improved photosynthetic efficiency. Meanwhile, characteristics of the cellular microenvironment could initiate various polymerization reactions inside living cells, including oxidative thiol cross-linking, condensation polymerization, and free radical polymerization. These reactions can be selectively conducted with reactive species generated in tumor cells, and the resulting polymers showed prolonged retention inside cells for modulating cell behaviors. Further development of cell-mediated polymerization strategies would provide an innovative platform for research and applications of multifunctional biomaterials and engineered biohybrid systems.

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ROS-initiated in-situ polymerization of diacetylene-containing lipidated peptide amphiphile in living cells

Cited by 9 publications

References 58 publications

Morphological Transformation and In Situ Polymerization of Caspase‐3 Responsive Diacetylene‐Containing Lipidated Peptide Amphiphile for Self‐Amplified Cooperative Antitumor Therapy

Morphological Transformation and In Situ Polymerization of Caspase‐3 Responsive Diacetylene‐Containing Lipidated Peptide Amphiphile for Self‐Amplified Cooperative Antitumor Therapy

Synthesizing biomaterials in living organisms

Polymer Materials Synthesized through Cell-Mediated Polymerization Strategies for Regulation of Biological Functions

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