ROP11 GTPase is a Negative Regulator of Multiple ABA Responses in <i>Arabidopsis</i>

Li, Zixing; Kang, Jun; Sui, Ning; Liu, Dong

doi:10.1111/j.1744-7909.2012.01100.x

Cited by 48 publications

(64 citation statements)

References 35 publications

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“…In this study, we evaluated the drought resistance of 14 transgenic Arabidopsis-overexpressing PYLs driven by the constitutive 35S cauliflower mosaic virus (CaMV) promoter, the stress-inducible RD29A promoter, the guard cell-specific GC1 and ROP11 promoters (20,21), and the green tissue-specific ribulose bisphosphate carboxylase small subunit (RBCS) RBCS-1A promoter (22). We found that, relative to the WT and all other combinations, pRD29A::PYL9 transgenic Arabidopsis plants had both greater drought resistance and accelerated drought-or ABA-induced leaf senescence.…”

Section: Significancementioning

confidence: 99%

ABA receptor PYL9 promotes drought resistance and leaf senescence

Zhao

Chan

Gao

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

483

402

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Drought stress is an important environmental factor limiting plant productivity. In this study, we screened drought-resistant transgenic plants from 65 promoter-pyrabactin resistance 1-like (PYL) abscisic acid (ABA) receptor gene combinations and discovered that pRD29A::PYL9 transgenic lines showed dramatically increased drought resistance and drought-induced leaf senescence in both Arabidopsis and rice. Previous studies suggested that ABA promotes senescence by causing ethylene production. However, we found that ABA promotes leaf senescence in an ethylene-independent manner by activating sucrose nonfermenting 1-related protein kinase 2s (SnRK2s), which subsequently phosphorylate ABA-responsive element-binding factors (ABFs) and Related to ABA-Insensitive 3/VP1 (RAV1) transcription factors. The phosphorylated ABFs and RAV1 up-regulate the expression of senescence-associated genes, partly by up-regulating the expression of Oresara 1. The pyl9 and ABA-insensitive 1-1 single mutants, pyl8-1pyl9 double mutant, and snrk2.2/3/6 triple mutant showed reduced ABA-induced leaf senescence relative to the WT, whereas pRD29A::PYL9 transgenic plants showed enhanced ABA-induced leaf senescence. We found that leaf senescence may benefit drought resistance by helping to generate an osmotic potential gradient, which is increased in pRD29A::PYL9 transgenic plants and causes water to preferentially flow to developing tissues. Our results uncover the molecular mechanism of ABA-induced leaf senescence and suggest an important role of PYL9 and leaf senescence in promoting resistance to extreme drought stress.drought stress | abscisic acid | PYL | dormancy | Arabidopsis C ell and organ senescence causes programmed cell death to regulate the growth and development of organisms. In plants, leaf senescence increases the transfer of nutrients to developing and storage tissues. Recently, studies on transgenic tobacco showed that delayed leaf senescence increases plant resistance to drought stress (1). However, the senescence and abscission of older leaves and subsequent transfer of nutrients are known to increase plant survival under abiotic stresses, including drought, low or high temperatures, and darkness (2, 3). Senescence mainly develops in an age-dependent manner and is also triggered by environmental stresses and phytohormones, such as abscisic acid (ABA), ethylene, salicylic acid, and jasmonic acid, but delayed by cytokinin (4).Senescence-associated genes (SAGs) are induced by leaf senescence. The expression of SAGs is tightly controlled by several senescence-promoting, plant-specific NAC (NAM, ATAF1, and CUC2) transcription factors, such as Oresara 1 (ORE1) (5), Oresara 1 sister 1 (ORS1) (6), and AtNAP (7). Environmental stimuli and phytohormones may regulate leaf senescence through NACs. Phytochrome-interacting factor 4 (PIF4) and PIF5 transcription factors promote dark-induced senescence by activating ORE1 expression (8). The expression of ORE1, AtNAP, and OsNAP (ortholog of AtNAP) is up-regulated by ABA by an unknown molecular m...

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Section: Significancementioning

confidence: 99%

ABA receptor PYL9 promotes drought resistance and leaf senescence

Zhao

Chan

Gao

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

483

402

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“…Note. When this paper was under preparation, another group reported that ROP11 interacted with ABI1 and regulated ABA responses (34,35), although authors found that ABI1 interaction with ROP11 occurs when the regulatory domain of ABI1 is deleted (35). It seems likely that different cross-talk nodes are present in subcellular compartments, such as the nucleus and cytosol, where they link alternate signals to ABA.…”

Section: Methodsmentioning

confidence: 99%

FERONIA receptor kinase pathway suppresses abscisic acid signaling in Arabidopsis by activating ABI2 phosphatase

Qian

Nibau

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

226

283

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Plant growth and development are controlled by a delicate balance of hormonal cues. Growth-promoting hormones and growth-inhibiting counterparts often antagonize each other in their action, but the molecular mechanisms underlying these events remain largely unknown. Here, we report a cross-talk mechanism that enables a receptor-like kinase, FERONIA (FER), a positive regulator of auxin-promoted growth, to suppress the abscisic acid (ABA) response through activation of ABI2, a negative regulator of ABA signaling. The FER pathway consists of a FER kinase interacting with guanine exchange factors GEF1, GEF4, and GEF10 that, in turn, activate GTPase ROP11/ARAC10. Arabidopsis mutants disrupted in any step of the FER pathway, including fer, gef1gef4gef10, or rop11/arac10, all displayed an ABAhypersensitive response, implicating the FER pathway in the suppression mechanism. In search of the target for the FER pathway, we found that the ROP11/ARAC10 protein physically interacted with the ABI2 phosphatase and enhanced its activity, thereby linking the FER pathway with the inhibition of ABA signaling.A-type protein phosphatase 2C | signal transduction | small GTPase

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“…Moreover, phenotypic analysis of rop10rop11 double mutant showed that they act in parallel pathways in ABA-mediated responses. Li et al ( 2012aLi et al ( , 2012b, using luciferase complementation imaging (LCI), Y2H assay, and co-immunoprecipitation assay, showed that activated ROP11 binds ABI1 (ABA insensitive), a PP2C phosphatase, and allows the downstream SnRKs to be activated, which further activate TFs and upregulate stress-related genes like RAD29 .…”

Section: Rac/rop Gtpases In Abiotic Stressmentioning

confidence: 99%

Small and Large G Proteins in Biotic and Abiotic Stress Responses in Plants

Pandey

Sharma

Pandey

2015

Elucidation of Abiotic Stress Signaling in Plants

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The sessile nature of plants has enabled them to develop a higher level of plasticity in adaptation against the hostile environmental conditions such as biotic and abiotic stresses. The intricate molecular machineries in plants, consisting of signal sensors, signal transducers, and generation of response, are highly similar with animal's signal transduction components; however, a large number of pathways also show a high level of divergence. G proteins are a part of signal transduction components, which play pivotal role in regulating a large number of physiological and developmental processes in both plants and animals. G proteins or guanine nucleotide-binding proteins serve as molecular switches, existing in two forms: an active conformation, when bound to GTP, and an inactive conformation, when bound to GDP. In active conformation, G proteins interact with downstream effector molecules to initiate various cellular responses. Based on their structure, they have been divided into heterotrimeric and monomeric G proteins, also called large and small G proteins, respectively, where the former is composed of three subunits (α, β, and γ) and latter is composed of single subunit (α). Plant growth and development is partially regulated by the combined activity of large and small G proteins. Besides, identifi cation of profuse number of stress-related genes interacting with monomeric and heterotrimeric G-protein complex in both Arabidopsis and rice suggests their involvement in modulation of abiotic stress responses. Further, based on the functional analysis of several G proteins (large and small) regulating cell growth, cell proliferation, seed germination, sugar sensing, and hormonal responses indicate these proteins are equally essential for plant development. Due to increased demand of food crops to sustain growing population in adverse environmental conditions, the role of G proteins and modulation of GTPase signaling has been studied in plants to produce genetically modifi ed crop plants with increased stress tolerance and productivity.

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ROP11 GTPase is a Negative Regulator of Multiple ABA Responses in Arabidopsis

Cited by 48 publications

References 35 publications

ABA receptor PYL9 promotes drought resistance and leaf senescence

ABA receptor PYL9 promotes drought resistance and leaf senescence

FERONIA receptor kinase pathway suppresses abscisic acid signaling in Arabidopsis by activating ABI2 phosphatase

Small and Large G Proteins in Biotic and Abiotic Stress Responses in Plants

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