Root-Secreted Coumarins and the Microbiota Interact to Improve Iron Nutrition in Arabidopsis

Harbort, Christopher J.; Hashimoto, Masayoshi; Inoue, Haruhiko; Niu, Yulong; Guan, Rui; Rombolà, Adamo Domenico; Kopriva, Stanislav; Voges, Mathias J.E.E.E.; Sattely, Elizabeth S.; Garrido‐Oter, Ruben; Schulze‐Lefert, Paul

doi:10.1016/j.chom.2020.09.006

Cited by 254 publications

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“…Besides their function in the mobilization and uptake of iron from the soil environment, coumarins such as scopoletin, possess a selective antimicrobial activity (Stassen et al 2020;Stringlis et al 2019a). Using the mutant f6'h1, which is impaired in the coumarin biosynthesis gene FERULOYL-CoA 6'HYDROXYLASE1, it was shown that F6'H1-dependent coumarins have a significant effect on the composition of microbiota in the rhizosphere or in gnotobiotic conditions using microbial synthetic communities (Harbort et al 2020;Stringlis et al 2018b;Voges et al 2019). Interestingly, WCS417 was shown to be highly tolerant to the antimicrobial activity of the coumarin scopoletin, while the soil-borne fungal pathogens F. oxysporum and Verticillium dahliae were strongly inhibited in their growth when exposed to scopoletin in the growth medium (Stringlis et al 2018b).…”

Section: Among the Stars: Wcs417 In A Root Microbiome Contextmentioning

confidence: 99%

Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

et al. 2020

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Background Since the 1980s, numerous mutualistic Pseudomonas spp. strains have been used in studies on the biology of plant growth-promoting rhizobacteria (PGPR) and their interactions with host plants. In 1988, a strain from the Pseudomonas fluorescens group, WCS417, was isolated from lesions of wheat roots growing in a take-all disease-suppressive soil. In subsequent trials, WCS417 limited the build-up of take-all disease in field-grown wheat and significantly increased wheat yield. In 1991, WCS417 was featured in one of the first landmark studies on rhizobacteria-induced systemic resistance (ISR), in which it was shown to confer systemic immunity in carnation (Dianthus caryophyllus) against Fusarium wilt. The discovery that WCS417 conferred systemic immunity in the model plant species Arabidopsis thaliana in 1996 incited intensive research on the molecular mechanisms by which PGPR promote plant growth and induce broad-spectrum disease resistance in plants. Since then, the strain name appeared in over 750 studies on beneficial plant-microbe interactions. Scope In this review, we will highlight key discoveries in plant-microbe interactions research that have emerged from over 30 years of research featuring WCS417 as a model rhizobacterial strain. WCS417 was instrumental in improving our understanding of the microbial determinants that are involved in root colonization and the establishment of mutually beneficial interactions with the host plant. The model strain also provided novel insight into the molecular mechanisms of plant growth promotion and the onset and expression of rhizobacteria-ISR. More recently, WCS417 has been featured in studies on host immune evasion during root colonization, and chemical communication in the rhizosphere during root microbiome assembly. Conclusions Numerous studies on the modes of action of WCS417 have provided major conceptual advances in our understanding of how free-living mutualists colonize the rhizosphere, modulate plant immunity, and promote plant growth. The concepts may prove useful in our understanding of the molecular mechanisms involved in other binary plant-beneficial microbe interactions, and in more complex microbial community contexts, such as the root microbiome.

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Section: Among the Stars: Wcs417 In A Root Microbiome Contextmentioning

confidence: 99%

Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

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“…In the “unavailable iron” condition, iron(III) (supplied as FeCl 3 ) is sparingly soluble and is unavailable to plants. This is critical for assessing nutritionally beneficial host-microbiota interactions, which are precluded in artificial systems where iron is removed altogether ( Harbort et al., 2020 ). As a control “available iron” medium with the same iron content and pH, the media is supplied with the same concentration of a chelated form of iron (FeEDTA, Ferric sodium ethylenediaminetetraacetate), which has increased solubility even in these alkaline conditions.…”

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confidence: 99%

“…Example datasets and scripts for analysis from ( Harbort et al., 2020 ) have been deposited to Mendeley Data with the https://doi.org/10.17632/tkdn6zbw7k.1 .…”

Section: Resource Availabilitymentioning

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“…This system provides a framework that can be adapted to study plant-microbiota interactions in further nutritional contexts. For complete details on the use and execution of this protocol, please refer to Harbort et al. (2020) .…”

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“…For complete details on the use and execution of this protocol, please refer to Harbort et al. (2020) .…”

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A gnotobiotic growth assay for Arabidopsis root microbiota reconstitution under iron limitation

et al. 2020

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Summary We present a gnotobiotic system for microbiota reconstitution on Arabidopsis thaliana under contrasting iron availability. This system induces iron starvation in plants by providing an unavailable form, mimicking conditions in alkaline soils. Inoculation of taxonomically diverse bacteria reconstitutes plants with a synthetic microbiota, allowing observation of nutrient-dependent interactions with commensals. Experimental optimization, including media composition and preparation of seedlings and bacteria, is discussed. This system provides a framework that can be adapted to study plant-microbiota interactions in further nutritional contexts. For complete details on the use and execution of this protocol, please refer to Harbort et al. (2020) .

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Microbiome‐assisted Agriculture

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Biocontrol of Plant Disease

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Root-Secreted Coumarins and the Microbiota Interact to Improve Iron Nutrition in Arabidopsis

Cited by 254 publications

References 64 publications

Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

A gnotobiotic growth assay for Arabidopsis root microbiota reconstitution under iron limitation

Microbiome‐assisted Agriculture

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