Root membrane lipids as potential biomarkers to discriminate silage‐corn genotypes cultivated on podzolic soils in boreal climate

Nadeem, Muhammad; Thomas, Raymond; Adigun, Oludoyin Adeseun; Manful, Charles F.; Wu, Jiaxu; Pham, Thu Huong; Zhu, Xiashi; Galagedara, Lakshman; Cheema, Mumtaz

doi:10.1111/ppl.13181

“…2c, 3c) were generated for the lipids with influential loadings accounting for the genotype and treatment segregation to further classify the treatments based on the altered membrane lipidome following infection. The cut-off value for variables important in projection (VIP) scores was defined as >1 (Nadeem et al 2020, Ravipati et al 2015). The 22 important root membrane lipid molecular species and 21 important stem membrane lipid molecular species were selected based on VIP scores greater than 1.…”

Section: Resultsmentioning

confidence: 99%

“…The method of lipid analysis was as described previously (Nadeem et al 2020). Lipids extracted from the soybean roots and stems were separated using an Accucore C30 reverse phase (C30RP) column (150 × 2 mm I.D., particle size: 2.6 µm, pore diameter: 150 Å; ThermoFisher Scientific, ON, Canada) applying the following solvent system: Solvent A (40: 60 v/v H 2 O and acetonitrile), and Solvent B (1:10: 90 v/v/v water: acetonitrile: isopropanol).…”

Section: Methods Of Lipid Extractionmentioning

confidence: 99%

Plant lipid metabolism in susceptible and tolerant soybean (Glycine max) cultivars in response to Phytophthora sojae colonization and infection

Adigun

¹

,

Pham

²

,

Nadeem

³

et al. 2021

Preprint

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Soybean is one of the most cultivated crops globally and a staple food for much of the world's population. The annual global crop losses due to infection by the Phytophthora sojae are currently estimated at approximately $2B USD, yet we have limited understanding of the role of lipid metabolism in the adaptative strategies used to limit infection and crop loss. We employed a multi-modal lipidomics approach to investigate how soybean cultivars remodel their lipid metabolism to successfully limit infection by Phytophthora sojae. Both the tolerant and susceptible soybean cultivars showed alterations in lipid metabolism in response to Phytophthora sojae infection. Relative to non-inoculated controls, induced accumulation of stigmasterol was observed in the susceptible cultivar whereas, induced accumulation of phospholipids and glycerolipids occurred in tolerant soybean cultivar. We have generated a comprehensive metabolic map of susceptible and tolerant soybean root and stem lipid metabolism to identify lipid modulators of host immune or tolerance response to Phytophthora sojae infection and identified potential pathways and unique lipid biomarkers like TG(15:0/22:0/22:5), TG(10:0/10:0/10:0), TG(10:0/10:0/14:0), DG(18:3/18:3), DG(16:0/18:3) and DG(24:0/18:2) as possible targets for the development of future plant protection solutions.

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“…(Zhao, Chang, Toh, & Arvan, 2007). Phosphatidic acid regulates phosphorylation of proteins, translation and transcription in biosynthesis of glycolipids, proliferation of cells and growth (Huang & Frohman, 2007;Nadeem et al, 2019;Nadeem et al, 2020). Survival, cell multiplication and reproduction along with plant-pathogen interactions are linked to the functionality of F I G U R E 3 Basic structure of phospholipids (PLs) and common head groups.…”

Section: Role Of Phospholipase Dmentioning

confidence: 99%

“…They also function in the modulation of cell physiological and phenotypic outcomes such as disease susceptibility or resistance (Zhao, Chang, Toh, & Arvan, 2007). Phosphatidic acid regulates phosphorylation of proteins, translation and transcription in biosynthesis of glycolipids, proliferation of cells and growth (Huang & Frohman, 2007; Nadeem et al, 2019; Nadeem et al, 2020). Survival, cell multiplication and reproduction along with plant–pathogen interactions are linked to the functionality of phosphatidic acid and PLD.…”

Section: Role and Regulation Of Phospholipids In Plant Defense Mechanmentioning

confidence: 99%

Recent advances in bio‐chemical, molecular and physiological aspects of membrane lipid derivatives in plant pathology

Adigun

¹

,

Nadeem

²

,

Pham

³

et al. 2020

Plant Cell & Environment

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Plant pathogens pose a significant threat to the food industry and food security accounting for 10-40% crop losses annually on a global scale. Economic losses from plant diseases are estimated at $300B for major food crops and are associated with reduced food availability and accessibility and also high food costs. Although strategies exist to reduce the impact of diseases in plants, many of these introduce harmful chemicals to our food chain. Therefore, it is important to understand and utilize plants' immune systems to control plant pathogens to enable more sustainable agriculture. Lipids are core components of cell membranes and as such are part of the first line of defense against pathogen attack. Recent developments in omics technologies have advanced our understanding of how plant membrane lipid biosynthesis, remodelling and/or signalling modulate plant responses to infection. Currently, there is limited information available in the scientific literature concerning lipid signalling targets and their biochemical and physiological consequences in response to plant pathogens. This review focusses on the functions of membrane lipid derivatives and their involvement in plant responses to pathogens as biotic stressors. We describe major plant defense systems including systemic-acquired resistance, basal resistance, hypersensitivity and the gene-for-gene concept in this context. 1 | INTRODUCTION The advancement in global agricultural production, the food industry and food security necessitates consideration of the impact of infectious pathogens on plants. This is because pathogens are widely recognized as significant obstacles to important and dependable food systems (Savary et al., 2019). Recent reports have demonstrated that plant diseases pose a significant threat to the food industry and to food security accounting for 10 to 40% crop losses annually on a global scale. Economic losses from plant diseases are estimated at $300B for major food crops, and diseases are associated with reduced food production, availability and accessibility as well as high food costs (Fletcher et al., 2006; Savary et al., 2019). Plants face different biotic stresses during their life cycle. For instance, a variety of diseases are caused by fungi, bacteria, protozoa, nematodes, viruses and phytoplasmas. These pathogens change favourable growing environments for plants into unfavourable conditions, particularly during susceptible growth stages. These cause significant yield losses both in greenhouses and under field conditions. Therefore, it is important to understand and utilize plants' innate immune systems to control plant pathogens to enable more sustainable agriculture (Brackin, Atkinson, Sturrock, & Rasmussen, 2017). Natural defense mechanisms involve a variety of signalling events and responses, which serve to combat intruding pathogens. The defense mechanism is categorized into constitutive and induced defense mechanisms. As the first line of defense, constitutive mechanisms utilize pre-formed chemicals and barriers such as ce...

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“…Lipid metabolites can also function as intracellular and extracellular signal mediators 18,20 . Plant lipids include glycerophospholipids (GPL), phytosterols (PST), sphingolipids (SGL), glycoglycerolipids (GGL) and glycerolipids (GL) 4,21,22 , and their metabolites are actively involved in plant defence responses against pathogen colonization 23,24 . They play important roles in the formation of the membrane interface between plant and microbial pathogen 23,24 .…”

Section: Introductionmentioning

confidence: 99%

Plant lipid metabolism in response to Phytophthora sojae infection in susceptible and tolerant soybean cultivars

Adigun

¹

,

Pham²,

Nadeem

³

et al. 2021

Preprint

Self Cite

0

View full text Add to dashboard Cite

Soybean is one of the most cultivated crops globally and a staple food for much of the world's population. The annual global crop losses due to infection by the Phytophthora sojae are currently estimated at approximately $2B USD, yet we have limited understanding of the role of lipid metabolism in the adaptative strategies used to limit infection and crop loss. We employed a multi-modal lipidomics approach to investigate how soybean cultivars remodel their lipid metabolism to successfully limit infection by Phytophthora sojae. Both the tolerant and susceptible soybean cultivars showed alterations in lipid metabolism in response to Phytophthora sojae infection. Relative to non-inoculated controls, induced accumulation of stigmasterol was observed in the susceptible cultivar whereas, induced accumulation of phospholipids and glycerolipids occurred in tolerant soybean cultivar. We have generated a comprehensive metabolic map of susceptible and tolerant soybean root and stem lipid metabolism to identify lipid modulators of host immune or tolerance response to Phytophthora sojae infection and identified potential pathways and unique lipid biomarkers like TG(15:0/22:0/22:5), TG(10:0/10:0/10:0), TG(10:0/10:0/14:0), DG(18:3/18:3), DG(16:0/18:3) and DG(24:0/18:2) as possible targets for the development of future plant protection solutions.

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Root membrane lipids as potential biomarkers to discriminate silage‐corn genotypes cultivated on podzolic soils in boreal climate

Cited by 7 publications

References 51 publications

Plant lipid metabolism in susceptible and tolerant soybean (Glycine max) cultivars in response to Phytophthora sojae colonization and infection

Plant lipid metabolism in susceptible and tolerant soybean (Glycine max) cultivars in response to Phytophthora sojae colonization and infection

Recent advances in bio‐chemical, molecular and physiological aspects of membrane lipid derivatives in plant pathology

Plant lipid metabolism in response to Phytophthora sojae infection in susceptible and tolerant soybean cultivars

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