Root explant produces multiple shoot from pericycle in Psoralea corylifolia – a leprosy destroyer medicinal plant

Jani, Jigar N.; Jha, Suman Kumar; Nagar, Durga Singh

doi:10.1016/j.indcrop.2015.02.001

Cited by 11 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…of genetic instability than by the other regeneration routes, and it is a more reliable method for clonal propagation 9,10 . Many endangered magnolia species with high ornamental or commercial value, such as M. dealbata and M. punduana, have been protected and expanded by in vitro propagation [11][12][13] .…”

mentioning

confidence: 99%

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

Cui

Deng

Zheng

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Magnolia sirindhorniae Noot. & Chalermglin is an endangered species with high ornamental and commercial value that needs to be urgently protected and judiciously commercialized. In this study, a protocol for efficient regeneration of this species is standardized. The lateral buds of the M. sirindhorniae plant were used as an explant. Half-strength Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 6-benzyladenine (BA), 0.1 mg/L α-naphthaleneacetic acid (NAA), and 2.0 mg/L gibberellic acid (GA 3 ) was found to be the optimal medium for shoot induction. The maximum shoot multiplication rate (310%) was obtained on Douglas-fir cotyledon revised medium (DCR) fortified with 0.2 mg/L BA, 0.01 mg/L NAA, and additives. The half-strength DCR medium supplemented with 0.5 mg/L NAA and 0.5 mg/L indole-3-butyric acid (IBA) supported the maximum rate (85.0%) of in vitro root induction. After a simple acclimatization process, the survival rate of plantlets in a substrate mixture of sterile perlite and peat soil (1:3; v/v ) was 90.2%. DNA markers were used for assessment of genetic uniformity, confirming the genetic uniformity and stability of regenerated plants of M. sirindhorniae . Thus, the described protocol can safely be applied for large scale propagation of this imperative plant.

show abstract

mentioning

confidence: 99%

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

Cui

Deng

Zheng

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Higher concentration of PG resulted in direct root formation in Tinospora cordifolia. The promotive effect of PG on rooting has been identified in several plant species [15,19,16]. PG has been reported in adventitious root formation in micropropagated shoots of adult wild cherry, In Vitro multiplication of Tebernaemontana fuchsiaefolia L. and direct shoot bud formation in Psoralea corylifolia [23,24,16].…”

Section: Discussionmentioning

confidence: 99%

“…The promotive effect of PG on rooting has been identified in several plant species [15,19,16]. PG has been reported in adventitious root formation in micropropagated shoots of adult wild cherry, In Vitro multiplication of Tebernaemontana fuchsiaefolia L. and direct shoot bud formation in Psoralea corylifolia [23,24,16]. The beneficial effect of PG is due to its ability to depress the peroxidase activity within the cultured explants, thereby protecting the endogenous auxin from peroxidation catalyzed oxidation [25,26].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Phloroglucinol Plays Role in Shoot Bud Induction and In Vitro Tuberization in Tinospora Cordifolia- A Medicinal Plant with Multi- Therapeutic Application

Kumar¹

2015

Adv Tech Biol Med

View full text Add to dashboard Cite

This paper shows the potential of phloroglucinol for In Vitro regeneration and tuberization of microshoots of Tinospora cordifolia. Within a week of nodal explants culture, axillary bud in nodal segments sprouted in all the media investigated including basal MS media without any growth regulators. Maximum response (52.2 per cent) evinced by MS media supplemented with 6.98 µM Kin. The same media produced maximum response for multiple shoot formation out of total shoot bud sprouted fostering average 3 shoots with 3.9 cm shoot length and 4.2 number of leaves per shoot. Effect of PG also investigated for mass multiplication and In Vitro rooting. Basal MS + 6.98 µM Kin + 79.4 µM PG increased shoot bud induction from 52.2 per cent to 84.8 per cent and multiple shoot production per cent from 12.9 to as high as 60.3 percent and also supported maximum number of shoot per explants (7.5). The same treatment promoted highest axillary shoot proliferation in terms of shoot length (3.9 cm) and number of leaves per shoot (4.3). Phloglucinol induced In Vitro tuberization in microshoots. Maximum rooting response observed in ½ MS medium supplemented with 7.4 µM IBA and 793.7 µM PG with 81.1 culture responding taking just 8.7 days to sprout and producing maximum number of roots (3.2) with an average length (3.1 cm). Rooted explants successfully hardened in Soil: Vermicompost: Vermiculite (1:1:1) with 100% success.

show abstract

“…Plant tissue culture has the potential to produce many clonal seedlings in a short time throughout the year, aiding in plant multiplication [ 6 , 7 ]. Direct multiple shoot induction is a more dependable technique for clonal multiplication and a valuable way to produce plantlets from young or mature plants with a reduced risk of genetic instability than by the other regeneration pathways [ 6 , 8 ]. The in vitro process’s success depends on a number of variables, including plant growth regulators and medium composition [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Propagation Technology for the Endangered Aquatic Species Nymphoides coronata

Lin,

Kang,

et al. 2024

Plants

View full text Add to dashboard Cite

Nymphoides coronata is an endangered aquatic plant species with significant medicinal and ecological importance. To preserve N. coronata from going extinct, we need to provide seedlings and efficient multiplication techniques so that it can be extensively studied. This study aimed to identify the most suitable sterilization treatment, growth medium, and substrate for the cultivation and propagation of N. coronata. Ethanol sterilization, fungicide treatment, and sterile water washing were the most important sterilization steps. A combination of 6-benzylaminopurine (6-BA) and indoleacetic acid (IAA) was the most suitable medium for bud induction and shoot proliferation. The use of α-naphthaleneacetic acid (NAA) increased the rooting rate and rooting time compared to indole-3-butyric acid (IBA). Increasing the concentration of NAA from 0.5 to 1.0 mg/L increased the rooting rate from 78 to 100% and reduced the rooting time from 7 to 5 days. The survival rate of N. coronata seedlings was 100% in a mixture of red soil and sand (1:1, w/w). As a result, the procedure mentioned above could potentially be used to safely propagate this rare species on a large scale. These findings provide valuable insights into the optimal conditions for the successful cultivation and propagation of N. coronata, which can contribute to the conservation and sustainable use of this important rare plant species.

show abstract

Root explant produces multiple shoot from pericycle in Psoralea corylifolia – a leprosy destroyer medicinal plant

Cited by 11 publications

References 48 publications

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

Phloroglucinol Plays Role in Shoot Bud Induction and In Vitro Tuberization in Tinospora Cordifolia- A Medicinal Plant with Multi- Therapeutic Application

In Vitro Propagation Technology for the Endangered Aquatic Species Nymphoides coronata

Contact Info

Product

Resources

About