Root endophytic fungus Piriformospora indica improves drought stress adaptation in barley by metabolic and proteomic reprogramming

Ghaffari, Mohammad Reza; Mirzaei, Mehdi; Ghabooli, Mehdi; Khatabi, Behnam; Wu, Yunqi; Zabet-Moghaddam, Masoud; Mohammadi-Nejad, Ghasem; Haynes, Paul A.; Hajirezaei, Mohammad; Sepehri, Mozhgan; Salekdeh, Ghasem Hosseini

doi:10.1016/j.envexpbot.2018.10.002

Cited by 95 publications

(64 citation statements)

References 41 publications

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“…Invertase catalyzes the hydrolysis of sucrose into glucose and fructose, plays a crucial role in primary metabolism and plant development. Down-regulation of cell wall invertase and sucrose synthase was reported from Piriformospora indica-inoculated barley exposed to drought stress compared to non-inoculated plants [52]. Our results imply a crucial role of SS and invertase in Lolium genotypes in the regulation of sugar biosynthesis when exposed to drought stress.…”

Section: Discussionsupporting

confidence: 57%

“…Our results show that the abundance of GS and GOGAT decreased in the two commercial genotypes under stress, but no change was observed in the abundance of these two enzymes in the S10 genotype. Similarly, Ghaffari et al [52] reported that drought stress declined NR, GS, and GOGAT enzymes in non-inoculated barley, but this was not the case for inoculated plants with Piriformospora indica. It was demonstrated in numerous studies that leaf nitrogen reduces progressively as the drought proceeds, and this can be correlated to photosynthetic machinery damage [71,69].…”

Section: Discussionmentioning

confidence: 96%

“…However, in the S10 genotype under drought stress, the antioxidant system did not respond as vigorously as expected, except for SOD and GST enzymes, which were enhanced to play defensive roles against ROS. Ghaffari et al [52] reported that four isoforms of GST and three isoforms of ascorbate peroxidase were up-regulated only in the non-inoculated barley; but not in the inoculated ones with mycorrhiza. Glutathione S-transferase was also up-regulated in all three genotypes of Lolium.…”

Section: Discussionmentioning

confidence: 99%

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Comparative physiological and proteomic analysis indicates lower shock response to drought stress conditions in a self-pollinating perennial ryegrass

Vanani¹,

Shabani²,

Rahimmalek³

et al. 2020

PLoS ONE

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We investigated the physiological and proteomic changes in the leaves of three Lolium perenne genotypes, one Iranian putative self-pollinating genotype named S10 and two commercial genotypes of Vigor and Speedy, subjected to drought stress conditions. The results of this study indeed showed higher RWC (relative water content), SDW (shoot dry weight), proline, ABA (abscisic acid), nitrogen and amino acid contents, and antioxidant enzymes activities of S10 under drought stress in comparison with the two other genotypes. A total of 915 proteins were identified using liquid chromatography-mass spectrometry (LC/MS) analysis, and the number of differentially abundant proteins between normal and stress conditions was 467, 456, and 99 in Vigor, Speedy, and S10, respectively. Proteins involved in carbon and energy metabolism, photosynthesis, TCA cycle, redox, and transport categories were up-regulated in the two commercial genotypes. We also found that some protein inductions, including those involved in amino acid and ABA metabolisms, aquaporin, HSPs, photorespiration, and increases in the abundance of antioxidant enzymes, are essential responses of the two commercial genotypes to drought stress. In contrast, we observed only slight changes in the protein profile of the S10 genotype under drought stress. Higher homozygosity due to self-pollination in the genetic background of the S10 genotype may have led to a lower variation in response to drought stress conditions.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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Comparative physiological and proteomic analysis indicates lower shock response to drought stress conditions in a self-pollinating perennial ryegrass

Vanani¹,

Shabani²,

Rahimmalek³

et al. 2020

PLoS ONE

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“…Ghaffari et al. also explored the molecular mechanisms underlying S. indica ‐mediated salt tolerance in rice by integrating high throughput shotgun proteomic and metabolomics data showing that S. indica ‐mediated salt tolerance altered primary metabolism to provide ATP and deal with the oxidative stress by minimizing the production of ROS under salinity. It is shown that S. indica enhanced the activity of proteins involved in the photosystem and the electron transfer chain, together with the accumulation of protector proteins in photorespiration, energy modulation, transporters, and autophagy.…”

Section: Symbiotic Proteomics In the Nonlegumes Plantsmentioning

confidence: 99%

“…Taken together, proteomic analysis has been used to dissect the molecular linkage between root endophytes and improved host tolerance. Proteomics could explain the impact of endophytes in enhancing the plant's antioxidative capacity via the activation of cellular antioxidant defense enzymes involved in the glutathione–ascorbate cycle, and various other functional groups of proteins, including photosynthesis, proteins involved in translation and degradation, energy production, signal transduction, and cell wall rearrangement …”

Section: Symbiotic Proteomics In the Nonlegumes Plantsmentioning

confidence: 99%

Plant–Microbe Symbiosis: What Has Proteomics Taught Us?

et al. 2019

Self Cite

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Beneficial microbes have a positive impact on the productivity and fitness of the host plant. A better understanding of the biological impacts and underlying mechanisms by which the host derives these benefits will help to address concerns around global food production and security. The recent development of omics-based technologies has broadened our understanding of the molecular aspects of beneficial plant-microbe symbiosis. Specifically, proteomics has led to the identification and characterization of several novel symbiosis-specific and symbiosis-related proteins and post-translational modifications that play a critical role in mediating symbiotic plant-microbe interactions and have helped assess the underlying molecular aspects of the symbiotic relationship. Integration of proteomic data with other "omics" data can provide valuable information to assess hypotheses regarding the underlying mechanism of symbiosis and help define the factors affecting the outcome of symbiosis. Herein, an update is provided on the current and potential applications of symbiosis-based "omic" approaches to dissect different aspects of symbiotic plant interactions. The application of proteomics, metaproteomics, and secretomics as enabling approaches for the functional analysis of plant-associated microbial communities is also discussed. 1 of 20) www.advancedsciencenews.com www.proteomics-journal.com to refer to the nodule-forming bacteria of the Rhizobium-and Frankia-type as true symbionts for plants. Other types of beneficial bacteria are referred to as plant growth promoting bacteria (PGPBs), which include endophytes or associative ectophytes that are much more variable in terms of their beneficial contributions. Over the past decades, proteomic approaches have been particularly successful in identifying numerous sets of host and symbiont-related and/or responsive proteins, and components of the signaling factors, which regulate and promote symbiosisrelated processes. Proteomics has also facilitated the discovery of the molecular basis of symbioses initiation and maintenance, and nutrient exchange between the symbiotic partners. (This review focuses on current proteomic applications used to investigate plant-microbe symbiosis such as the large-scale analysis of key regulators of symbiotic-related proteins and their post-translational modifications (PTMs). We also review protein changes at the cellular level, PTMs, and the associated interactions with other molecules during plant symbiotic relationships, with a particular emphasis on the developmental steps of legume-rhizobia symbiosis. The review also links data from proteomics and other "omic" approaches to provide a comprehensive understanding of the molecular basis of plant-microbe symbiosis.

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Cactus Endophytic Fungi and Bioprospecting for their Enzymes and Bioactive Molecules: A Systematic Review

de Menezes Souza,

de Menezes Fonseca,

Pinheiro de Souza

et al. 2023

Chemistry & Biodiversity

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Endophytic fungi are associated with plant health and represent a remarkable source of potential of enzymes and bioactive compounds, but the diversity of endophytes remains uncertain and poorly explored, especially in Cactaceae, one of the most species‐rich families adapted to growing in arid and semi‐arid regions. The aim of this study was to conduct a systematic review on the diversity and bioprospecting of endophytic fungi from Cactaceae. We analysed peer‐reviewed articles from seven databases using PRISMA guidelines. The results showed that the Cactaceae family is a source of new taxa, but the diversity of endophytic fungi of Cactaceae is little explored, mainly the diversity among tissues and by metagenomics. Bioprospecting studies have shown that these microorganisms can be used in the production of enzymes and larvicidal and antifungal compounds. Our results are relevant as a starting point for researchers to develop studies that expand the knowledge of plant mycobiota in arid and semi‐arid ecosystems, as well as comprising a remarkable source of fungal compounds with several biotechnological applications.

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Root endophytic fungus Piriformospora indica improves drought stress adaptation in barley by metabolic and proteomic reprogramming

Cited by 95 publications

References 41 publications

Comparative physiological and proteomic analysis indicates lower shock response to drought stress conditions in a self-pollinating perennial ryegrass

Comparative physiological and proteomic analysis indicates lower shock response to drought stress conditions in a self-pollinating perennial ryegrass

Plant–Microbe Symbiosis: What Has Proteomics Taught Us?

Cactus Endophytic Fungi and Bioprospecting for their Enzymes and Bioactive Molecules: A Systematic Review

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