Root Dentin Morphology After Different Modes of Citric Acid and Tetracycline Hydrochloride Conditioning

Abstract

The purpose of this study was to assess the effect of citric acid and tetracycline HCl application to dentin surfaces by a "passive dripping" or an "active burnishing" technique. Twenty dentin blocks were prepared from freshly extracted non-diseased human impacted third molars. The blocks were root planed and randomly assigned to two groups for treatment with either citric acid or tetracycline HCl. The duration of treatment was 30, 60, 120, or 240 seconds. Control blocks were treated with distilled water. After treatment the blocks were processed for observation and measurements in the scanning electron microscope (SEM). Application of either of the acid solutions resulted in removal of the smear layer. Measurements indicated a time dependent increase in the mean dentinal tubule orifice diameter ranging from 1.05 microns in control specimens to 3.18 microns after 4 minutes treatment (citric acid group). The increase in tubule diameter was significantly greater (P less than or equal to 0.01) for both citric acid treatment modalities than tetracycline HCl treatment. There was also a time dependent increase in the depth of penetration as measured by a trumpeting of the tubule profiles, and this penetration was significantly greater (P less than or equal to 0.01) after citric acid treatments. Passive or active application of the acids did not seem to have any major impact on the measurements or on the surface morphology. It was concluded that citric acid causes more extensive changes than tetracycline HCl and that the mode of application of the agent is probably not critical.

Effects of Citric Acid and EDTA on Periodontally Involved Root Surfaces: A SEM Study

“…The results of this study were similar to the studies done by Hanes PJ and Labahn R. 4,6 However, this study is in contrast to studies done by Lafferty and Lasho where the dentinal tubules and the dentin between the tubules were affected to the same degree by either of the acid solutions. 7,8 Intergroup comparison showed citric acid had greater mean total surface area occupied by the dentinal tubules than EDTA.…”

Gingivarecessies en parodontale plastische chirurgie

“…Daarmee worden de collagene vezels blootgelegd. Het doel hiervan is de epitheliale groei te vertragen en de aanhechting van bindweefsel te bevorderen (Labahn et al, 1992). Er is echter nooit aangetoond dat deze maatregelen het uiteindelijke resultaat van de recessiebedekking verbeteren (Wennström, 1996;Cairo et al, 2009;Chambrone en Tatakis, 2015).…”

Efeito da desmineralização óssea sobre parâmetros de superfície e sobre o comportamento de pré-osteoblastos em cultura: estudo em microscopia eletrônica de varredura e confocal

“…O uso de ácidos para desmineralizar tecidos duros é bastante antigo, tendo sido amplamente documentado em biomodificação de superfícies de dentina e cemento visando potencializar os processos reparativos em tratamentos de defeitos periodontais (SELVIG et al, 1981;BAL;EREN;BALOS, 1990;HERITIER, 1983;LABAHN et al, 1992;PITARU et al, 1984b;HALS, 1977). O condicionamento ácido de superfícies radiculares tem sido defendido por proporcionar remoção da smear layer resultante da raspagem (REGISTER, 1973;REGISTER;BURDICK, 1975;BLOMLÖF et al, 2000;HERITIER, 1983), descontaminação da superfície (BAL; EREN; BALOS, 1990), liberação de fatores que atuam na cicatrização dos tecidos periodontais (POLSON; PROYE, 1983) e criação de um substrato que privilegia a seleção de células nos eventos de reparação (REGISTER, 1973;REGISTER;BURDICK, 1975;PITARU et al, 1984a;PITARU et al,1984b).…”

“…remodelação óssea e eventual substituição óssea (GRAYSON et al, 2015 de dentina e cemento (SELVIG et al, 1981;BAL;EREN;BALOS, 1990;HERITIER, 1983;LABAHN et al, 1992;PITARU et al, 1984a;PITARU et al, 1984b;HALS, 1977), visando potencializar os processos reparativos em tratamentos cirúrgicos periodontais.…”