Root cap size and shape influence responses to the physical strength of the growth medium in Arabidopsis thaliana primary roots

Roue, Juliette; Chauvet, Hugo; Brunel-Michac, Nicole; Bizet, François; Moulia, Bruno; Badel, Éric; Legue;, Valerie

doi:10.1093/jxb/erz418

Cited by 22 publications

(37 citation statements)

References 32 publications

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“…Such a plant root phenotype did not occur in the Litterbox system ( Figure 1G) which, instead, exhibited root growth similar to soil-grown plants. Furthermore, roots on the agar were noticeably curled ( Figure 1H), which is likely linked to a continuous root growth reorientation by the environment-sensing root cap [61]. Predominant growth on top of the agar might be linked to a humidity gradient with the highest water potential on top of the agar, potentially caused by the PDMS sheet [62].…”

Section: Resultsmentioning

confidence: 99%

Litterbox—A gnotobiotic Zeolite-Clay System to Investigate Arabidopsis–Microbe Interactions

et al. 2020

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Figure S1: Optimisation of the Litterbox system. A. Effect of zeolite amount on plant weight (fresh weight aboveground plant parts) of six-week-old plants grown on coarse (35 g or 70 g) zeolite covered by fine ground (20 g) zeolite. B. Effect of zeolite granularity on plant weight of six-week-old plants grown on 90 g of zeolite (70 + 20 g and 90 g). C. Effect of MES buffer (2.5 mM) on plant weight of six-week-old plants grown on coarse zeolite (70 g) covered by fine ground zeolite (20 g). Tukey's boxplots. Figure S2: Effect of agar concentration and tip size of agar filled pipette tips on plant growth. A. Plant weight of six-week-old plants (fresh weight aboveground plant parts) of plants sown either on cut 10 μl or 200 μl pipette tips filled with either 0.6% or 1% agar. Seedlings were transferred seven days after sowing to 70 g coarse zeolite covered by 20 g fine ground zeolite. B. Percentage of plants grown to full size per transplanted seedling. Filled circles represent sample mean, error bars depict standard deviation, dots mark individual plants, thick bar represents median, dotted bars represent quartiles, two-way ANOVA.

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Section: Resultsmentioning

confidence: 99%

Litterbox—A gnotobiotic Zeolite-Clay System to Investigate Arabidopsis–Microbe Interactions

et al. 2020

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“…Most of the roots grew on top of the agar ( Figure 1H), which is unnatural and did not occur in the Litterbox system ( Figure 1G). Furthermore, roots on the agar were noticeably curled ( Figure 1H), which is likely linked to a continuous root growth reorientation by the environment-sensing root cap [62] . Predominant growth on top of the agar might be linked to a humidity gradient with the highest water potential on top of the agar, potentially caused by the PDMS sheet [63] .…”

Section: Resultsmentioning

confidence: 99%

Litterbox - A gnotobiotic zeolite-clay system to investigate Arabidopsis-microbe interactions

Miebach

Schlechter

Clemens

et al. 2020

Preprint

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Plants are colonised by millions of microorganisms representing thousands of species with varying effects on plant growth and health. The microbial communities found on plants are compositionally consistent and their overall positive effect on the plant is well known. However, the effects of individual microbiota members on plant hosts and vice versa , as well as the underlying mechanisms remain largely unknown. Here, we describe 'Litterbox', a highly controlled system to investigate plant-microbe interactions. Plants were grown gnotobiotically on zeolite-clay, an excellent soil replacement that retains enough moisture to avoid subsequent watering. Plants grown on zeolite phenotypically resemble plants grown under environmental conditions. Further, bacterial densities on leaves in the Litterbox system resembled those in temperate environments. A PDMS sheet was used to cover the zeolite, thereby significantly lowering the bacterial load in the zeolite and rhizosphere. This reduced the likelihood of potential systemic responses in leaves induced by microbial rhizosphere colonisation. We present results of example experiments studying the transcriptional responses of leaves to defined microbiota members and the spatial distribution of bacteria on leaves. We anticipate that this versatile and affordable plant growth system will promote microbiota research and help in elucidating plant-microbe interactions and their underlying mechanisms. Introduction:Plants offer three different habitats to microbes: the endosphere, the rhizosphere, and the phyllosphere. The endosphere encompasses the habitat formed by internal tissues of plants, whereas the rhizosphere and phyllosphere encompass the surfaces of belowground and aboveground plant organs, respectively. Plants host remarkably diverse and complex, yet structured, microbial communities, collectively referred to as the plant microbiota [1][2][3] . Due to the microbiota's diversity and complexity, it is not surprising that the traditional view of host-microbe interactions focussing on plant pathogens, nitrogen-fixing rhizobacteria, and phosphate-mobilizing mycorrhizal fungi, has recently shifted to a holistic view considering the plant and its associated microbiota as a metaorganism or holobiont [4][5][6] . It is widely recognised that members of the microbiota assist in nutrient uptake, promote growth, and protect against biotic and abiotic stresses [7][8][9][10][11][12][13][14] . To harness these positive impacts of plant-associated microbiota, the use of synthetic microbiota has been proposed [15][16][17] . The prospect of using synthetic microbial communities to promote sustainable agriculture is leading to a growing appreciation of plant microbiota research.Generally, plant microbiota research aims to understand 1) plant-microbe and microbe-microbe interactions ranging from the individual microorganism to the microbial community resolution, 2) the underlying molecular mechanisms of these interactions, and 3) their contribution to microbial community structure. Thus far...

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“…To investigate the ability of Arabidopsis roots to progress in a medium with varying strength, Roué et al [69] designed an experimental setup consisting of two layers of growth medium containing Phytagel at different concentrations. The lower layer has a higher concentration and is consequently more resistant to penetration, mimicking a more compact soil layer.…”

Section: Localized Versus Distant Responsesmentioning

confidence: 99%

“…The lower layer has a higher concentration and is consequently more resistant to penetration, mimicking a more compact soil layer. Upon contact with the interface between the two layers, the root either penetrates the lower layer or reorients its growth [69]. Arabidopsis genotypes exhibiting contrasted root cap morphologies have different penetration abilities.…”

Section: Localized Versus Distant Responsesmentioning

confidence: 99%

Between Stress and Response: Function and Localization of Mechanosensitive Ca2+ Channels in Herbaceous and Perennial Plants

Hartmann

Tinturier

Julien

et al. 2021

IJMS

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Over the past three decades, how plants sense and respond to mechanical stress has become a flourishing field of research. The pivotal role of mechanosensing in organogenesis and acclimation was demonstrated in various plants, and links are emerging between gene regulatory networks and physical forces exerted on tissues. However, how plant cells convert physical signals into chemical signals remains unclear. Numerous studies have focused on the role played by mechanosensitive (MS) calcium ion channels MCA, Piezo and OSCA. To complement these data, we combined data mining and visualization approaches to compare the tissue-specific expression of these genes, taking advantage of recent single-cell RNA-sequencing data obtained in the root apex and the stem of Arabidopsis and the Populus stem. These analyses raise questions about the relationships between the localization of MS channels and the localization of stress and responses. Such tissue-specific expression studies could help to elucidate the functions of MS channels. Finally, we stress the need for a better understanding of such mechanisms in trees, which are facing mechanical challenges of much higher magnitudes and over much longer time scales than herbaceous plants, and we mention practical applications of plant responsiveness to mechanical stress in agriculture and forestry.

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Root cap size and shape influence responses to the physical strength of the growth medium in Arabidopsis thaliana primary roots

Abstract: Analysis of the growth and orientation of roots of Arabidopsis mutants with differing root cap sizes and shapes indicates that the form of the cap affects root responses to variations in the strength of the growth medium.

Cited by 22 publications

References 32 publications

Litterbox—A gnotobiotic Zeolite-Clay System to Investigate Arabidopsis–Microbe Interactions

Litterbox—A gnotobiotic Zeolite-Clay System to Investigate Arabidopsis–Microbe Interactions

Litterbox - A gnotobiotic zeolite-clay system to investigate Arabidopsis-microbe interactions

Between Stress and Response: Function and Localization of Mechanosensitive Ca2+ Channels in Herbaceous and Perennial Plants

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