Root Bacteria Recruited by Phragmites australis in Constructed Wetlands Have the Potential to Enhance Azo-Dye Phytodepuration

Riva, Valentina; Mapelli, Francesca; Syranidou, Evdokia; Crotti, Elena; Choukr‐Allah, Redouane; Kalogerakis, Nicolas; Borin, Sara

doi:10.3390/microorganisms7100384

Cited by 34 publications

(19 citation statements)

References 82 publications

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“…Finally, the biofilm production capacity of the isolates, as a proxy for root colonization, was evaluated by a colorimetric assay based on crystal violet staining ( Riva et al, 2019a ), and the results were evaluated according to the method of Stepanović et al (2007) .…”

Section: Methodsmentioning

confidence: 99%

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

et al. 2021

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Global population growth and climate change raise a challenge to agriculture, which, combined with the issues concerning the use of chemical fertilizers, have generated increasing attention in the use of plant-associated bacteria as a sustainable strategy in agri-food systems. The objective of this study is to evaluate the ability of five bacterial strains, previously isolated from the rhizosphere or endosphere of plants adapted to harsh environmental conditions, to act as potential plant biofertilizers in different conditions of water availability. The strain biosafety for a deliberate environmental release was investigated through a literature survey and antibiotic resistance testing. The selected strains were first characterized for their plant growth–promoting (PGP) and rhizocompetence-related traits through in vitro assays and then on short-term in vivo experiments on tomato plants. A long-term greenhouse experiment was further conducted to monitor the PGP effect of the bacteria during the entire life cycle of tomato plants subjected to full irrigation or to severe water deficit conditions, aiming to assess their actual effect on plant productivity, which is the ultimate target of the agricultural sector. Some of the strains showed a potential in improving water use efficiency and mitigating plant water stress. Under severe irrigation deficit, four of the tested strains, Micrococcus yunnanensis M1, Bacillus simplex RP-26, Pseudomonas stutzeri SR7-77, and Paenarthrobacter nitroguajacolicus 2–50, significantly increased the number of productive plants in comparison to non-bacterized control ones. Two of them, Bacillus simplex RP-26 and Paenarthrobacter nitroguajacolicus 2–50, demonstrated also, under full irrigation, to significantly improve the water productivity in comparison with non-bacterized plants. Despite all the strains showed promising PGP potential in short-term assays, the positive effect of the bacterial inoculants on plant physiology and fruit yield was observed in some cases but never corroborated by statistical significance. These results highlight the importance of performing long-term in vivo experiments to define the real PGP ability of a bacterial inoculant to positively impact plant production.

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Section: Methodsmentioning

confidence: 99%

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

et al. 2021

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“…Each decimal dilution was plated in triplicate to count the colony-forming unit (cfu) and calculate the cultivable bacteria abundance (cfu/g) present in each fraction. Medium 869 has been proposed among the optimal media for the isolation of endophytes [ 30 , 31 ], and it was also successfully applied to isolate rhizosphere bacteria [ 41 ]. Colonies were randomly picked after two days of incubation at 30 °C and were spread three times on the same medium to obtain pure cultures.…”

Section: Methodsmentioning

confidence: 99%

“…We established a collection of 371 bacterial isolates from the root system (E-R-SSR fractions) of A. spinosa, its residuesphere and the bulk soil, using the same cultivation medium for all the fractions in order to compare their composition in terms of cultivable bacteria taxonomy. With this aim, we selected a medium previously applied for the isolation of both endophytes and rhizospheric bacteria [30,31,41]. We avoided media mimicking the specific conditions of the different niches (e.g., endosphere, rhizosphere, soil) which, by the application of selecting cultivation conditions (e.g., the use of saline medium) could hamper the comparison between the niche sub-collections.…”

Section: Phylogenetic Classification and Distribution Of The Cultivabmentioning

confidence: 99%

Unveiling the Microbiota Diversity of the Xerophyte Argania spinosa L. Skeels Root System and Residuesphere

et al. 2020

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The microbiota associated to xerophyte is a “black box” that might include microbes involved in plant adaptation to the extreme conditions that characterize their habitat, like water shortage. In this work, we studied the bacterial communities inhabiting the root system of Argania spinosa L. Skeels, a tree of high economic value and ecological relevance in Northern Africa. Illumina 16S rRNA gene sequencing and cultivation techniques were applied to unravel the bacterial microbiota’s structure in environmental niches associated to argan plants (i.e., root endosphere, rhizosphere, root-surrounding soil), not associated to the plant (i.e., bulk soil), and indirectly influenced by the plant being partially composed by its leafy residue and the associated microbes (i.e., residuesphere). Illumina dataset indicated that the root system portions of A. spinosa hosted different bacterial communities according to their degree of association with the plant, enriching for taxa typical of the plant microbiome. Similar alpha- and beta-diversity trends were observed for the total microbiota and its cultivable fraction, which included 371 isolates. In particular, the residuesphere was the niche with the highest bacterial diversity. The Plant Growth Promotion (PGP) potential of 219 isolates was investigated in vitro, assessing several traits related to biofertilization and biocontrol, besides the production of exopolysaccharides. Most of the multivalent isolates showing the higher PGP score were identified in the residuesphere, suggesting it as a habitat that favor their proliferation. We hypothesized that these bacteria can contribute, in partnership with the argan root system, to the litter effect played by this tree in its native arid lands.

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“…Isolation of effective reactive dye degrading bacteria was performed from soil samples collected from the rhizosphere region (Riva et al, 2019; Sinha et al, 2018) of the paddy fields in the Brahmapuram village in Vellore, Tamil Nadu, India (12.9165°N, 79.1325°E). A total of 12 soil samples were collected by random sampling procedure (Shah et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

Biodegradation and biosorption of Reactive Red 120 dye by immobilized Pseudomonas guariconensis: Kinetic and toxicity study

Reddy

Osborne

2020

Water Environment Research

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Reactive dyes are pernicious pollutants in textile effluent, which are to be treated passably before discharging into the environment. In the present study, a potential dye degrading bacterial strain Pseudomonas guariconensis was isolated from paddy rhizosphere and was characterized by 16S rRNA gene sequencing. The biodegradation ability of the strain was evaluated by time‐based study with immobilized bacterial cells in calcium alginate biocarrier matrix and also with free cells. The results indicated that the strain exhibited maximum degradation of 91% when immobilized in the biocarrier matrix. The enzymatic study revealed the production of oxidoreductase enzymes. The degraded products were identified as 2‐amino‐3‐phenylpropanoic acid and benzoquinone by gas chromatography–mass spectroscopy (GC‐MS) analysis, and a degradative pathway was derived based on the enzymatic profile. A packed bed column was designed using P. guariconensis VITSAJ5 immobilized in calcium alginate beads as a biosorbent for the removal of Reactive Red 120. The immobilized bacterial cells exhibited 87% uptake of RR120, whereas the nonimmobilized bacterial cells exhibited a maximum uptake of 37%. The phytotoxicity analysis by seed germination assay revealed an enhanced plumule and radicle length, indicating the nontoxic byproducts after the treatment of Reactive Red 120 by VITSAJ5 compared to the untreated Reactive Red 120 solution. Practitioner points Current study is the first report on Pseudomonas guariconensis capable of degrading reactive dyes (Reactive Red 120) It was observed that the degradation potential was maximum when cells were immobilized with Ca‐Ag biocarrier matrix Breakdown metabolism of Reactive Red 120 was derived through pathway prediction Employing immobilized bacteria in a packed bed column found to possess a prominent biosorption ability on the matrix enhancing the degradation process Toxic reactive dye was converted into nontoxic compounds, evidenced by phytotoxicity studies

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Root Bacteria Recruited by Phragmites australis in Constructed Wetlands Have the Potential to Enhance Azo-Dye Phytodepuration

Cited by 34 publications

References 82 publications

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

Unveiling the Microbiota Diversity of the Xerophyte Argania spinosa L. Skeels Root System and Residuesphere

Biodegradation and biosorption of Reactive Red 120 dye by immobilized Pseudomonas guariconensis: Kinetic and toxicity study

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