Root-Associated Bacterial Community Shifts in Hydroponic Lettuce Cultured with Urine-Derived Fertilizer

Gerrewey, Thijs Van; El‐Nakhel, Christophe; Pascale, Stefania De; Paepe, Jolien De; Clauwaert, Peter; Kerckhof, Frederiek‐Maarten; Boon, Nico; Geelen, Danny

doi:10.3390/microorganisms9061326

Cited by 13 publications

(7 citation statements)

References 167 publications

(143 reference statements)

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“…The statistical analyses were carried out as described before by Van Gerrewey, El‐Nakhel, et al. ( 2021 ). In summary, all the statistical analyses were performed in R (v. 4.1.0) using the phyloseq package (v. 1.36.0) to import the data (McMurdie & Holmes, 2013 ; R Core Team, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Bacterially enhanced plant‐growing media for controlled environment agriculture

Van Gerrewey,

Navarrete,

Vandecruys

et al. 2024

Microbial Biotechnology

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Microbe–plant interactions in the root zone not only shape crop performance in soil but also in hydroponic cultivation systems. The biological and physicochemical properties of the plant‐growing medium determine the root‐associated microbial community and influence bacterial inoculation effectiveness, which affects plant growth. This study investigated the combined impact of plant‐growing media composition and bacterial community inoculation on the root‐associated bacterial community of hydroponically grown lettuce (Lactuca sativa L.). Ten plant‐growing media were composed of varying raw materials, including black peat, white peat, coir pith, wood fibre, composted bark, green waste compost, perlite and sand. In addition, five different bacterial community inocula (BCI S1–5) were collected from the roots of lettuce obtained at different farms. After inoculation and cultivation inside a vertical farm, lettuce root‐associated bacterial community structures, diversity and compositions were determined by evaluating 16S rRNA gene sequences. The study revealed distinct bacterial community structures among experimental replicates, highlighting the influence of raw material variations on root‐associated bacterial communities, even at the batch level. However, bacterial community inoculation allowed modulation of the root‐associated bacterial communities independently from the plant‐growing medium composition. Bacterial diversity was identified as a key determinant of plant growth performance with green waste compost introducing Bacilli and Actinobacteria, and bacterial community inoculum S3 introducing Pseudomonas, which positively correlated with plant growth. These findings challenge the prevailing notion of hydroponic cultivation systems as sterile environments and highlight the significance of proper plant‐growing media raw material selection and bacterial community inoculation in shaping root‐associated microbiomes that provide stability through microbial diversity. This study supports the concept of creating bacterially enhanced plant‐growing media to promote plant growth in controlled environment agriculture.

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Section: Methodsmentioning

confidence: 99%

Bacterially enhanced plant‐growing media for controlled environment agriculture

Van Gerrewey,

Navarrete,

Vandecruys

et al. 2024

Microbial Biotechnology

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“…High throughput sequencing has enabled scientists to ascertain which organisms are prevalent in hydroponic systems. A study of the influence of urine-derived fertilizers conveyed the range of OTUs that are native to the hydroponic lettuce rhizosphere [ 97 ]. Pseudomonas was the only genus that was a true indicator organism, present in over 90% of samples.…”

Section: Plant Growth Promoting Bacteria Research In Hydroponicsmentioning

confidence: 99%

The Use of PGPB to Promote Plant Hydroponic Growth

Stegelmeier¹,

Rose²,

Joris³

et al. 2022

Plants

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Improvements to the world’s food supply chain are needed to ensure sufficient food is produced to meet increasing population demands. Growing food in soilless hydroponic systems constitutes a promising strategy, as this method utilizes significantly less water than conventional agriculture, can be situated in urban areas, and can be stacked vertically to increase yields per acre. However, further research is needed to optimize crop yields in these systems. One method to increase hydroponic plant yields involves adding plant growth-promoting bacteria (PGPB) into these systems. PGPB are organisms that can significantly increase crop yields via a wide range of mechanisms, including stress reduction, increases in nutrient uptake, plant hormone modulation, and biocontrol. The aim of this review is to provide critical information for researchers on the current state of the use of PGPB in hydroponics so that meaningful advances can be made. An overview of the history and types of hydroponic systems is provided, followed by an overview of known PGPB mechanisms. Finally, examples of PGPB research that has been conducted in hydroponic systems are described. Amalgamating the current state of knowledge should ensure that future experiments can be designed to effectively transition results from the lab to the farm/producer, and the consumer.

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“…And after harvesting, lettuce leaves will easy to appear yellowing, wilting even decay. Especially when the leaves decayed, it will not only affect appearance but also infects other good quality leaves, and even the nitrite content will sharply increase ( Yan et al., 2015 ; Van Gerrewey et al., 2021 ). These defective leaves will shorten the shelf life of lettuce and also can produce a certain degree of commodity value loss.…”

Section: Introductionmentioning

confidence: 99%

“…and even the nitrite content will sharply increase (Yan et al, 2015;Van Gerrewey et al, 2021). These defective leaves will shorten the shelf life of lettuce and also can produce a certain degree of commodity value loss.…”

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confidence: 99%

Hydroponic lettuce defective leaves identification based on improved YOLOv5s

Jin,

Jiao,

Zhang

et al. 2023

Front. Plant Sci.

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Achieving intelligent detection of defective leaves of hydroponic lettuce after harvesting is of great significance for ensuring the quality and value of hydroponic lettuce. In order to improve the detection accuracy and efficiency of hydroponic lettuce defective leaves, firstly, an image acquisition system is designed and used to complete image acquisition for defective leaves of hydroponic lettuce. Secondly, this study proposed EBG_YOLOv5 model which optimized the YOLOv5 model by integrating the attention mechanism ECA in the backbone and introducing bidirectional feature pyramid and GSConv modules in the neck. Finally, the performance of the improved model was verified by ablation experiments and comparison experiments. The experimental results proved that, the Precision, Recall rate and mAP0.5 of the EBG_YOLOv5 were 0.1%, 2.0% and 2.6% higher than those of YOLOv5s, respectively, while the model size, GFLOPs and Parameters are reduced by 15.3%, 18.9% and 16.3%. Meanwhile, the accuracy and model size of EBG_YOLOv5 were higher and smaller compared with other detection algorithms. This indicates that the EBG_YOLOv5 being applied to hydroponic lettuce defective leaves detection can achieve better performance. It can provide technical support for the subsequent research of lettuce intelligent nondestructive classification equipment.

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Root-Associated Bacterial Community Shifts in Hydroponic Lettuce Cultured with Urine-Derived Fertilizer

Cited by 13 publications

References 167 publications

Bacterially enhanced plant‐growing media for controlled environment agriculture

Bacterially enhanced plant‐growing media for controlled environment agriculture

The Use of PGPB to Promote Plant Hydroponic Growth

Hydroponic lettuce defective leaves identification based on improved YOLOv5s

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