Room-temperature single-photon emission from zinc oxide nanoparticle defects and their <i>in vitro</i> photostable intrinsic fluorescence

Chung, Kelvin; Karle, Timothy J.; Khalid, Asma; Abraham, Amanda N.; Shukla, Ravi; Gibson, Brant C.; Simpson, David A.; Djurišić, Aleksandra B.; Amekura, H.; Tomljenovic‐Hanic, Snjezana

doi:10.1515/nanoph-2015-0138

Cited by 20 publications

(14 citation statements)

References 65 publications

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“…The initial photobleaching (20 s) might be attributed to some impurities attached to the surface of eggs. A similar trend of in vitro fluorescence was seen inside the human keratinocyte cells with the ZnO nanoparticles 37 . Figure 6 shows that Ascaris lumbricoides have the brightest fluorophores on its outer surface showing emission counts of more than 2 M counts per second.…”

Section: Resultssupporting

confidence: 74%

Real-time detection and identification of nematode eggs genus and species through optical imaging

Qazi

Khalid

Poddar

et al. 2020

Sci Rep

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Nematode eggs are pervasive pathogens that infect billions of people and livestock every year. Adult parasitic nematode worms can be distinguished based on their size and morphology. However, their eggs, particularly their species Ascaris lumbricoides and Ascaris suum cannot be identified from each other. Identifying eggs of helminths from wastewater and sludge is important from a public health perspective to minimize the spread of Ascaris infections. Numerous methods exist for nematode identification, from a morphological-based approach to high throughput sequencing technology. However, these techniques are not consistent and often laborious and time-consuming. In this study, we demonstrate that non-invasive real-time identification of eggs is possible based on their intrinsic fluorescence. Using confocal microscopy, we investigate the autofluorescence properties of five species of nematode eggs and observe clear differences between genus and for the first time their species in sludge samples. This non-invasive imaging technique could lead to better understanding of these species and may assist in early control of diseases.

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Section: Resultssupporting

confidence: 74%

Real-time detection and identification of nematode eggs genus and species through optical imaging

Qazi

Khalid

Poddar

et al. 2020

Sci Rep

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“…The figure illustrates bright and stable emission from ball-milled NPs, within a range 35 k–0.5 M counts/s. The figure also shows an initial decay within the first few seconds attributed to partial photobleaching of cells [15]. This photobleaching is attributed to cell autofluorescence which diminishes in the first few seconds of exposure.…”

Section: Resultsmentioning

confidence: 99%

“…However, full and long term encapsulation is not suitable if the advantage of biodegradability and/or therapeutic properties of these NPs are to be used in biomedical applications. Recently, photostable in vitro single-photon emission has been observed from optical defects within ZnO NPs derived from ion implantation followed by thermal oxidation [15]. Even though, stable fluorescence of these defects has great potential for various applications, for their practical realization this material needs to be widely available.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible and Biodegradable Magnesium Oxide Nanoparticles with In Vitro Photostable Near-Infrared Emission: Short-Term Fluorescent Markers

et al. 2019

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Imaging of biological matter by using fluorescent nanoparticles (NPs) is becoming a widespread method for in vitro imaging. However, currently there is no fluorescent NP that satisfies all necessary criteria for short-term in vivo imaging: biocompatibility, biodegradability, photostability, suitable wavelengths of absorbance and fluorescence that differ from tissue auto-fluorescence, and near infrared (NIR) emission. In this paper, we report on the photoluminescent properties of magnesium oxide (MgO) NPs that meet all these criteria. The optical defects, attributed to vanadium and chromium ion substitutional defects, emitting in the NIR, are observed at room temperature in NPs of commercial and in-house ball-milled MgO nanoparticles, respectively. As such, the NPs have been successfully integrated into cultured cells and photostable bright in vitro emission from NPs was recorded and analyzed. We expect that numerous biotechnological and medical applications will emerge as this nanomaterial satisfies all criteria for short-term in vivo imaging.

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“…Moreover, it exhibits many crystalline defects that can emit visible light, such as oxygen or zinc vacancies (V O or V Zn ), oxygen or zinc interstitials (O i or Zn i ) or combinations of these single defects 4–6 . Some of them are so efficient an emitter that they can be used as single photon sources 7–9 . These defects emit from the blue to the red, with distinct PL QY.…”

Section: Introductionmentioning

confidence: 99%

Doping of ZnO inorganic-organic nanohybrids with metal elements

Zhang¹,

Apostoluk²,

Théron³

et al. 2019

Sci Rep

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We present a general and in-depth study of the effect of dopants in hybrid inorganic/organic ZnO/PAA (polyacrylic acid) nanocomposites. These dopants vary as much by their ionic size, as by their electronic valence and some of them have been used in ZnO due to their known magnetic and/or optical properties. The chemical nature of the dopants controls their ability to incorporate into ZnO crystal lattice. Three concentrations (0.1%, 1% and 5%) of dopants were studied in order to compare the effect of the concentration with the results obtained previously in the literature. Our results confirm in the first place the trend observed in the literature, that increase in dopant concentration leads to quenching of visible luminescence for ZnO nanocrystals obtained by very different processes. However, the degradation of photoluminescence quantum yield (PL QY) is not inevitable in our nanocomposites. At low doping concentration for some dopants with a small or comparable ionic radius than Zn 2+ , PL QY can be maintained or even improved, making it possible to tune the visible emission spectrum between 2.17 eV and 2.46 eV. This opens up the prospect of synthesizing phosphors without rare earth for white LEDs, whose spectrum can be tuned to render warm or cold white light, by a chemical synthesis process with a low environmental impact.

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Room-temperature single-photon emission from zinc oxide nanoparticle defects and their in vitro photostable intrinsic fluorescence

Cited by 20 publications

References 65 publications

Real-time detection and identification of nematode eggs genus and species through optical imaging

Real-time detection and identification of nematode eggs genus and species through optical imaging

Biocompatible and Biodegradable Magnesium Oxide Nanoparticles with In Vitro Photostable Near-Infrared Emission: Short-Term Fluorescent Markers

Doping of ZnO inorganic-organic nanohybrids with metal elements

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