Room-Temperature Harvesting Oxidase-Mimicking Enzymes with Exogenous ROS Generation in One Step

Abstract

Despite the advantages of low cost, high stability, and activities, a majority of nanozymes rely on strict synthesis conditions and precise size/structure control, hindering the stable, bulk, and high-yield production that is necessary for general use. To facilitate the transition of nanozymes from benchtop to real-world applications, we herein present a one-step approach, which only needs mixing of two broad commercialized reagents at room temperature, to harvest gold nanoparticles–bovine serum albumin (BSA) nanocomposite (BSA-Au) with distinct oxidase-like activity and good stability in a broad range of harsh conditions. Density functional theory (DFT) calculations demonstrate the oxidase-like activity of BSA-Au stemming from thermodynamically and kinetically favored facets for O2 activation. The reactive oxygen species (ROS) generation of BSA-Au contributes to the catalytic activities and further enables water sterilization and antibacterial applications against superbugs. This one-step strategy promises great potential in bulk production of nanozyme for broad application beyond laboratory use.

Porous, Oxygen Vacancy Enhanced CeO2–x Microspheres with Efficient Enzyme-Mimetic and Photothermal Properties

“…The signals marked by an asterix in the ESR spectrum obtained at zero-time disappeared significantly and the ESR spectrum became more symmetric at 20 min (Figure A,B). The spectrum obtained at 20 min is very similar to the ESR spectrum obtained for the DMPO–OOH spin adduct with six partially superimposed resonance signals which shows the presence of superoxide in the studied system. , The change of ESR spectrum with the time also shows that two or more radical species are responsible from the ESR spectrum of the CeO 2 –H 2 O 2 –CH 3 OH system. To determine the spectroscopic features of these radicals, ESR spectra of the CeO 2 –H 2 O 2 –CH 3 OH system recorded at different times were used for simulation via nonlinear least square solver fminsearch.m in MATLAB ® .…”

Antibacterial Nanomaterials: Mechanisms, Impacts on Antimicrobial Resistance and Design Principles

“…In terms of killing mechanisms, most antimicrobial nanomaterials interact with either multiple targets or specific conserved bacterial constituents that are hard to modify by gene mutations [18c] . The variable physicochemical properties of nanomaterials provide more opportunities for engineering design, [141] which can confer long‐lasting, controllable bactericidal effects and a high capability to prevent/degrade biofilms [142] . These features make antimicrobial nanomaterials promising candidates to kill resistant bacteria in biofilms and prevent AMR spread.…”

Sensitive colorimetric assay of T4 DNA ligase by the oxidase nanozyme of LaMnO3.26 coupled with a hyperbranched amplification reaction

“…The electron spin resonance (ESR) spectroscopy results further confirm this conclusion, and the ESR spectra are considered as direct evidence for the identification of ROS. 30 2,2,6,6-Tetramethylpiperidine (TEMP) traps 1 O 2 , 31 producing a 1 : 1 : 1 characteristic peak (Fig. 3C), indicating the production of 1 O 2 in the system.…”