Romanian Wormwood (Artemisia absinthium L.): Physicochemical and Nutraceutical Screening

Moacă, Elena-Alina; Pavel, Ioana Zinuca; Danciu, Corina; Crăiniceanu, Zorin; Minda, Daliana; Ardelean, Florina; Antal, Diana; Ghiulai, Roxana; Cioca, Andreea; Derban, Mihnea; Simu, Sebastian; Chioibaş, Raul; Szuhanek, Camelia; Dehelean, Cristina-Adriana

doi:10.3390/molecules24173087

Cited by 42 publications

(36 citation statements)

References 38 publications

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“…For the analysis of cell migration, we used cell Sense Dimension Software. To determine the migratory ability of the tumor cells, the scratch closure rate was calculated as previously described [ 31 ]:

where A t 0 is the scratch area at 0 h and A t is the scratch area at 24 h.…”

Section: Methodsmentioning

confidence: 99%

Biological Evaluation of Black Chokeberry Extract Free and Embedded in Two Mesoporous Silica-Type Matrices

et al. 2020

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Black chokeberry fruits possess a wide range of biological activities, among which the most important that are frequently mentioned in the literature are their antioxidant, anti-inflammatory, anti-proliferative, and antimicrobial properties. The present paper reports, for the first time, the encapsulation of the ethanolic extract of Aronia melanocarpa L. fruits into two mesoporous silica-type matrices (i.e., pristine MCM-41 and MCM-41 silica decorated with zinc oxide nanoparticles). The aim of this work was to evaluate the antiradicalic capacity, the antimicrobial potential, and the effects on the cell viability on a cancer cell line (i.e., A375 human melanoma cell line) versus normal cells (i.e., HaCaT human keratinocytes) of black chokeberry extract loaded on silica-type matrices in comparison to that of the extract alone. The ethanolic polyphenolic extract obtained by conventional extraction was characterized by high-performance liquid chromatography with a photodiode array detector (HPLC–PDA) and spectrophotometric methods. The extract was found to contain high amounts of polyphenols and flavonoids, as well as good radical scavenging activity. The extract-loaded materials were investigated by Fourier transform infrared spectroscopy, N2 adsorption–desorption isotherms, thermal analysis, and radical scavenger activity on solid samples. The black chokeberry extract, both free and loaded onto mesoporous silica-type matrices, exhibited a significant antioxidant capacity. Antibacterial activity was recorded only for Gram-positive bacteria, with a more potent antibacterial effect being observed for the extract loaded onto the ZnO-modified MCM-41 silica-type support than for the free extract, probably due to the synergistic effect of the ZnO nanoparticles that decorate the pore walls of silica. The cellular viability test (i.e., MTT assay) showed dose- and time-dependent activity regarding the melanoma cell line. The healthy cells were less affected than the cancer cells, with all tested samples showing good cytocompatibility at doses of up to 100 µg/mL. Improved in vitro antiproliferative and antimigratory (i.e., scratch assay) potential was demonstrated through the loading of black chokeberry extract into mesoporous silica-type matrices, and the screened samples exhibited low selectivity against the tested non-tumor cell line. Based on presented results, one can conclude that mesoporous silica-type matrices are good hosts for black chokeberry extract, increasing its antioxidant, antibacterial (on the screened strains), and in vitro antitumor (on the screened cell line) properties.

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where A t 0 is the scratch area at 0 h and A t is the scratch area at 24 h.…”

Section: Methodsmentioning

confidence: 99%

Biological Evaluation of Black Chokeberry Extract Free and Embedded in Two Mesoporous Silica-Type Matrices

et al. 2020

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“…This condition is characterized by hyperproliferation of keratinocytes and massive in ltration of white blood cells [28]. Although the pathogenesis of psoriasis has not been fully understood, there is evidence that IL-10, IL-6, and IFN-α produce many immuno-derived cytokines, including IL-23, IL-17A, IL-20, and IL-22, TNF-γ and TNF-α are involved in the pathogenesis and interaction network of psoriasis [29][30][31][32][33][34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Anti-Psoriasis Inflammation And Anti-Oxidation of The Extract From The Flower of Datura Metel L.

Wang¹,

Zhang²,

Wang³

et al. 2021

Preprint

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Background： At present, Flos Datura L. has been reported to have obvious anti-psoriasis effect, but its active ingredient is still unknown. Objectives: The chemical characteristics and anti-psoriasis inflammatory and antioxidant activities of partially purified flavonoids components of Flos Daturae (FC-FD) were investigated.Methods: FC-FD were analyzed by ultra-performance liquid chromatography (UPLC)-electrospray ionization (ESI)-quadrupole time-of-flight (Q-TOF)-mass spectrometry (MSE) combined, while antioxidant potency was monitored using a drosophila model. And the immune inflammatory response was measured in guinea pig animal model.Results: After comparison, 14 products were identified. In vitro antioxidant activity assays showed that FC-FD could effectively scavenge hydroxyl radicals. Furthermore, the longest survival time of the male and female Drosophila melanogaster was extended by 100.50% and 96.82%, respectively. In addition, in the psoriasiform guinea pigs model, FC-FD could decrease the histopathological score and the expression of PCNA protein, and block the production of TNF-α. Notably, the partial purified ﬂavonoids extracted from Flos Datura was and identiﬁed for the ﬁrst time. Conclusions: The results indicated that FC-FD are potential natural antioxidants in extending lifespan and anti-psoriatic inflammatory drugs.

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“…The experiment followed the rules and was approved by the Bioethical Committee of the Faculty of Pharmacy of Victor Babes University of Medicine and Pharmacy Timisoara, Romania (No. 1/13.01.2020) [44,45]. Five groups (n = 5), each consisting of 7 mice, were included in the study as follows: (i) control (surface~1 cm 2 -inner surface of the left ear); (ii) animals, to which the experimental animal model of inflammation was induced by using TPA dissolved in acetone (20 µL/mouse ear) (surface~1 cm 2 -inner surface of the right ear); (iii) animals, to which acetone (20 µL/mouse ear) was applied on the ear (surface~1 cm 2 -inner surface of the right ear); (iv) animals, to which indomethacin cream (4%) was typically applied as the treatment after the induction of the experimental animal model of inflammation (surface~1 cm 2 -inner surface of the right ear); (v) animals to which OB extract (300 µg/cm 2 ) was typically applied as the treatment after the induction of the experimental animal model of inflammation (surface~1 cm 2 -inner surface of the right ear) [46].…”

Section: In Vivo Tpa-induced Ear Inflammation Protocolmentioning

confidence: 99%

Phytochemical and Biological Screening of Oenothera biennis L. Hydroalcoholic Extract

et al. 2020

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Oenothera biennis L. (OB), also commonly known as evening primrose, belongs to the Onagraceae family and has the best studied biological activity of all the members in the family. In therapy, the most frequently used type of extracts are from the aerial part, which are the fatty oils obtained from the seeds and have a wide range of medicinal properties. The aim of this study was to evaluate the phytochemical composition and biological activity of OB hydroalcoholic extract and to provide directions for the antimicrobial effect, antiproliferative and pro-apoptotic potential against A375 melanoma cell line, and anti-angiogenic and anti-inflammatory capacity. The main polyphenols and flavonoids identified were gallic acid, caffeic acid, epicatechin, coumaric acid, ferulic acid, rutin and rosmarinic acid. The total phenolic content was 631.496 µgGAE/mL of extract and the antioxidant activity was 7258.67 μmolTrolox/g of extract. The tested extract had a mild bacteriostatic effect on the tested bacterial strains. It was bactericidal only against Candida spp. and S. aureus. In the set of experimental conditions, the OB extract only manifested significant antiproliferative and pro-apoptotic activity against the A375 human melanoma cell line at the highest tested concentration, namely 60 μg/mL. The migration potential of A375 cells was hampered by the OB extract in a concentration-dependent manner. Furthermore, at the highest tested concentration, the OB extract altered the mitochondrial function in vitro, while reducing the angiogenic reaction, hindering compact tumor formation in the chorioallantoic membrane assay. Moreover, the OB extract elicited an anti-inflammatory effect on the experimental animal model of ear inflammation.

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Romanian Wormwood (Artemisia absinthium L.): Physicochemical and Nutraceutical Screening

Cited by 42 publications

References 38 publications

Biological Evaluation of Black Chokeberry Extract Free and Embedded in Two Mesoporous Silica-Type Matrices

Biological Evaluation of Black Chokeberry Extract Free and Embedded in Two Mesoporous Silica-Type Matrices

Anti-Psoriasis Inflammation And Anti-Oxidation of The Extract From The Flower of Datura Metel L.

Phytochemical and Biological Screening of Oenothera biennis L. Hydroalcoholic Extract

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