Rolled graphene oxide foams as three-dimensional scaffolds for growth of neural fibers using electrical stimulation of stem cells

Akhavan, Omid; Ghaderi, Elham; Shirazian, Soheil A.; Rahighi, Reza

doi:10.1016/j.carbon.2015.06.079

Cited by 204 publications

(140 citation statements)

References 61 publications

(64 reference statements)

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“…Electrical coupling has also been proposed as a mechanism to explain the enhanced neuronal differentiation of NSCs in laminin-coated graphene (Park et al, 2011; Tang et al, 2013). In fact, electrical currents stimulate NSC differentiation into neurons (Chang et al, 2011a; Akhavan et al, 2016) and electrical activity is known to affect neuronal differentiation and survival in both the developing and adult nervous system (Spitzer, 2006, 2012). It could also be plausible that the topological patterns of the TRG with a characteristic wrinkle nanotexture might favor cell survival and oligodendrocyte morphological maturation.…”

Section: Discussionmentioning

confidence: 99%

“…In fact, scaffolds that support neural growth can be made of porous foams and membranes alone or loaded with a variety of GBNs and CNTs (Ramanathan et al, 2008; Chao et al, 2010; Alvarez et al, 2013; Li et al, 2013; Shah et al, 2014; Song et al, 2014; Weaver and Cui, 2015; Akhavan et al, 2016; Guo et al, 2016b,c). Moreover, there is evidence indicating that graphene and GBNs can serve as substrates for neural stem cells (NSC) differentiation, neuronal and oligodendrocyte growth, and the formation of neural circuits in cell culture ( in vitro) (Li et al, 2011, 2013; Park et al, 2011; Akhavan and Ghaderi, 2013a,b, 2014; Lorenzoni et al, 2013; Solanki et al, 2013; Tang et al, 2013; Akhavan et al, 2014, 2015; Shah et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

Defteralı

Verdejo

Majeed

et al. 2016

Front. Bioeng. Biotechnol.

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Graphene, graphene-based nanomaterials (GBNs), and carbon nanotubes (CNTs) are being investigated as potential substrates for the growth of neural cells. However, in most in vitro studies, the cells were seeded on these materials coated with various proteins implying that the observed effects on the cells could not solely be attributed to the GBN and CNT properties. Here, we studied the biocompatibility of uncoated thermally reduced graphene (TRG) and poly(vinylidene fluoride) (PVDF) membranes loaded with multi-walled CNTs (MWCNTs) using neural stem cells isolated from the adult mouse olfactory bulb (termed aOBSCs). When aOBSCs were induced to differentiate on coverslips treated with TRG or control materials (polyethyleneimine-PEI and polyornithine plus fibronectin-PLO/F) in a serum-free medium, neurons, astrocytes, and oligodendrocytes were generated in all conditions, indicating that TRG permits the multi-lineage differentiation of aOBSCs. However, the total number of cells was reduced on both PEI and TRG. In a serum-containing medium, aOBSC-derived neurons and oligodendrocytes grown on TRG were more numerous than in controls; the neurons developed synaptic boutons and oligodendrocytes were more branched. In contrast, neurons growing on PVDF membranes had reduced neurite branching, and on MWCNTs-loaded membranes oligodendrocytes were lower in numbers than in controls. Overall, these findings indicate that uncoated TRG may be biocompatible with the generation, differentiation, and maturation of aOBSC-derived neurons and glial cells, implying a potential use for TRG to study functional neuronal networks.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

Defteralı

Verdejo

Majeed

et al. 2016

Front. Bioeng. Biotechnol.

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“…The ultrastructure of the SH‐SY5Y cells on the surface of PM sheets was observed by SEM as described previously . Briefly, all samples of 5th day were fixed with 2.5% glutaraldehyde solution at 4°C for 12 h and washed twice with H 2 O.…”

Section: Methodsmentioning

confidence: 99%

In vivo feasibility test using transparent carbon nanotube‐coated polydimethylsiloxane sheet at brain tissue and sciatic nerve

Wang

Lee

et al. 2017

J Biomedical Materials Res

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Carbon nanotubes, with their unique and outstanding properties, such as strong mechanical strength and high electrical conductivity, have become very popular for the repair of tissues, particularly for those requiring electrical stimuli. Polydimethylsiloxane (PDMS)-based elastomers have been used in a wide range of biomedical applications because of their optical transparency, physiological inertness, blood compatibility, non-toxicity, and gas permeability. In present study, most of artificial nerve guidance conduits (ANGCs) are not transparent. It is hard to confirm the position of two stumps of damaged nerve during nerve surgery and the conduits must be cut open again to observe regenerative nerves after surgery. Thus, a novel preparation method was utilized to produce a transparent sheet using PDMS and multiwalled carbon nanotubes (MWNTs) via printing transfer method. Characterization of the PDMS/MWNT (PM) sheets revealed their unique physicochemical properties, such as superior mechanical strength, a certain degree of electrical conductivity, and high transparency. Characterization of the in vitro and in vivo usability was evaluated. PM sheets showed high biocompatibility and adhesive ability. In vivo feasibility tests of rat brain tissue and sciatic nerve revealed the high transparency of PM sheets, suggesting that it can be used in the further development of ANGCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1736-1745, 2017.

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“…The interaction between neuronal cells and biomaterials has recently raised increasing interest due to its great potential in the application field . In general, cell adhesion to synthetic surfaces influences their proliferation and differentiation according to the chemical and topographical properties of the substrate .…”

Section: Introductionmentioning

confidence: 99%

Micro‐Raman detection of the differentiation state of SH‐SY5Y cells grown on silicon and aluminium substrates

Ricci

Sagini

Caponi

et al. 2018

J Raman Spectroscopy

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SH‐SY5Y cell line is a model for neural diseases originating from neuroblastoma, a paediatric embryonal malignancy that may differentiate and regress to a benign state. By monitoring the differentiation in single living cells, it could be possible to reach a better understanding of the process and, consequently, to develop new therapeutic approaches. In this work, we investigated the potential of Raman microspectroscopy to monitor biochemical changes in single living SH‐SY5Y cells treated with a combination of retinoic acid (RA) and brain‐derived neurotrophic factor to stimulate the differentiation toward a neuronal phenotype. The results show that the spectroscopic analysis is able to detect biochemical alterations consistent with the differentiation process. Moreover, we investigated also the influence of the cell culture substrate on the treatment outcome. Silicon and aluminium are 2 materials suitable for the spectroscopic analysis and of interest in neuroscience applications. Even though silicon has an ensured biocompatibility, it is subject to alterations after the treatment with RA. Aluminium revealed to be a cost‐effective cell culture substrate for the RA and brain‐derived neurotrophic factor treatment of SH‐SY5Y cells and particularly suitable for Raman microspectroscopy.

show abstract

Rolled graphene oxide foams as three-dimensional scaffolds for growth of neural fibers using electrical stimulation of stem cells

Cited by 204 publications

References 61 publications

In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

In vivo feasibility test using transparent carbon nanotube‐coated polydimethylsiloxane sheet at brain tissue and sciatic nerve

Micro‐Raman detection of the differentiation state of SH‐SY5Y cells grown on silicon and aluminium substrates

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