Roles of the transcriptional regulators Fts1, <scp>YlNrg1</scp>, <scp>YlTup1</scp>, and <scp>YlSsn6</scp> in the repression of the yeast‐to‐filament transition in the dimorphic yeast <i>Yarrowia lipolytica</i>

Mao, Yi-Sheng; Chen, Jia-Wen; Wang, Zhenhua; Xu, Mengyang; Gao, Xiang-Dong

doi:10.1111/mmi.15017

Cited by 5 publications

(5 citation statements)

References 41 publications

(93 reference statements)

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“…They performed the deletion of the gen and RNA-seq analysis. Nrg1 turn out not to be a key player for the dimorphic switch, but the available transcriptomic data (40) proved to be crucial for the interpretation of our results. Among the genes repressed by Nrg1 are 5 encoding erythrose reductases: YALI0B07117g, YALI0B21780, YALI0A15906, YALI0D04092, YALI0E18348.…”

Section: Discussionmentioning

confidence: 90%

Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia lipolytica

Rzechonek,

Szczepańczyk,

Borodina

et al. 2024

Preprint

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Background Erythritol is a four-carbon polyol with an unclear role in metabolism of some unconventional yeasts. Its production has been linked to the osmotic stress response, but the mechanism of stress protection remains unclear. Additionally, erythritol can be used as a carbon source. In the yeast Yarrowia lipolytica, its assimilation is activated by the transcription factor Euf1. The study investigates whether this factor can link erythritol to other processes in the cell. Results The research was performed on two closely related strains of Y. lipolytica: MK1 and K1, where strain K1 has no functional Euf1. Cultures were carried out in erythrol-containing and erythrol-free media. Transcriptome analysis revealed the effect of Euf1 on the regulation of more than 150 genes. Some of these could be easily connected with different aspects of erythritol assimilation, such as: utilization pathway, a new potential isoform of transketolase, or polyol transporters. However, many of the upregulated genes have never been linked to metabolism of erythritol. The most prominent examples are the degradation pathway of branched-chain amino acids and the glyoxylate cycle. The high transcription of genes affected by Euf1 is still dependent on the erythritol concentration in the medium. Moreover, almost all up-regulated genes have an ATGCA motif in the promoter sequence. Conclusions These findings are particularly relevant given the increasing use of erythritol-induced promoters in genetic engineering of Y. lipolytica. Moreover, use of this yeast in biotechnological processes often takes place under osmotic stress conditions. Erythritol might be produce as a by-product, thus better understanding of its influence on cell metabolism could facilitate processes optimization.

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Section: Discussionmentioning

confidence: 90%

Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia lipolytica

Rzechonek,

Szczepańczyk,

Borodina

et al. 2024

Preprint

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“…6A and B). The transcript levels of MAR1 ( YALI0B02266g ) and HOY1 were repressed by Tup1 and Ssn6, both of which are transcriptional repressors involved in filamentous growth (40). These data suggest that Mar1 functions downstream of Tup1 and Ssn6 and upstream of Hoy1 in morphological regulation in Y. lipolytica .…”

Section: Discussionmentioning

confidence: 99%

“…lipolytica is a dimorphic yeast that changes its cell morphology between the yeast form and pseudohyphal or hyphal form in response to various environmental conditions, including pH and nutrition (34). The cell morphology is regulated by transcription factors, such as Mhy1 and Hoy1, that promote the transition from the yeast to hyphal form and by transcription factors, such as Znc1, Tec1, Fts1, and Nrg1, that promote the transition from the hyphal to yeast form (35)(36)(37)(38)(39)(40). Nonetheless, the entire picture of the transcriptional regulatory network of cell morphogenesis in Y. lipolytica remains to be established.…”

Section: Introductionmentioning

confidence: 99%

Mar1, an HMG-box protein, regulatesn-alkane adsorption and cell morphology of the dimorphic yeastYarrowia lipolytica

Kimura-Ishimaru,

Liang,

Matsuse

et al. 2024

Preprint

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The dimorphic yeastYarrowia lipolyticapossesses an excellent ability to utilizen-alkane as a sole carbon and energy source. Although there are detailed studies on the enzymes that catalyze the reactions in the metabolic processes ofn-alkane inY. lipolytica, the molecular mechanism underlying the incorporation ofn-alkane into the cells remains to be elucidated. BecauseY. lipolyticaadsorbsn-alkane, we postulated thatY. lipolyticaincorporatesn-alkane through direct interaction with it. We isolated and characterized mutants defective in adsorption ton-hexadecane. One of the mutants harbored a nonsense mutation inMAR1(Morphology andn-alkane Adsorption Regulator) encoding a protein containing a high mobility group box. The deletion mutant ofMAR1exhibited defects in adsorption ton-hexadecane and filamentous growth on solid media, whereas the strain that overexpressedMAR1exhibited hyperfilamentous growth. Fluorescence microscopic observations suggested that Mar1 localizes in the nucleus. RNA-seq analysis revealed the alteration of the transcript levels of several genes, including those encoding transcription factors and cell surface proteins, by the deletion ofMAR1. These findings suggest thatMAR1is involved in the transcriptional regulation of the genes required forn-alkane adsorption and cell morphology transition.

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“…They performed the deletion of the gene and RNA-seq analysis. Nrg1 turn out not to be a key player for the dimorphic switch, but the available transcriptomic data [ 41 ] proved to be crucial for the interpretation of our results. Among the genes repressed by Nrg1 there are 5 encoding erythrose reductases: YALI0B07117g, YALI0B21780, YALI0A15906, YALI0D04092, YALI0E18348 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia Lipolytica

Rzechonek,

Szczepańczyk,

Borodina

et al. 2024

Microb Cell Fact

View full text Add to dashboard Cite

Background Erythritol is a four-carbon polyol with an unclear role in metabolism of some unconventional yeasts. Its production has been linked to the osmotic stress response, but the mechanism of stress protection remains unclear. Additionally, erythritol can be used as a carbon source. In the yeast Yarrowia lipolytica, its assimilation is activated by the transcription factor Euf1. The study investigates whether this factor can link erythritol to other processes in the cell. Results The research was performed on two closely related strains of Y. lipolytica: MK1 and K1, where strain K1 has no functional Euf1. Cultures were carried out in erythritol-containing and erythritol-free media. Transcriptome analysis revealed the effect of Euf1 on the regulation of more than 150 genes. Some of these could be easily connected with different aspects of erythritol assimilation, such as: utilization pathway, a new potential isoform of transketolase, or polyol transporters. However, many of the upregulated genes have never been linked to metabolism of erythritol. The most prominent examples are the degradation pathway of branched-chain amino acids and the glyoxylate cycle. The high transcription of genes affected by Euf1 is still dependent on the erythritol concentration in the medium. Moreover, almost all up-regulated genes have an ATGCA motif in the promoter sequence. Conclusions These findings may be particularly relevant given the increasing use of erythritol-induced promoters in genetic engineering of Y. lipolytica. Moreover, use of this yeast in biotechnological processes often takes place under osmotic stress conditions. Erythritol might be produce as a by-product, thus better understanding of its influence on cell metabolism could facilitate processes optimization.

show abstract

Roles of the transcriptional regulators Fts1, YlNrg1, YlTup1, and YlSsn6 in the repression of the yeast‐to‐filament transition in the dimorphic yeast Yarrowia lipolytica

Cited by 5 publications

References 41 publications

Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia lipolytica

Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia lipolytica

Mar1, an HMG-box protein, regulatesn-alkane adsorption and cell morphology of the dimorphic yeastYarrowia lipolytica

Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia Lipolytica

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