Roles of the cytoskeleton in human diseases

Li, Mengxin; Li, Peng; Wang, Zhenming; Liu, Lijia; Cao, Mengjiao; Cui, Jingyao; Wu, Fanzi; Yang, Jing

doi:10.1007/s11033-022-08025-5

Cited by 6 publications

(4 citation statements)

References 100 publications

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“…Cytoskeleton mainly includes three highly abundant fibrillar proteins: microfilaments (F-actin), microtubules (α-and β-tubulin), and intermediate filaments (keratin and desmin), as well as numerous actin binding proteins (cofilin, twinfilin, profilin, spectrin, dystrophin, talin, integrin, annexin, and transgelin) and intermediate filaments-associated proteins (plectin), which play a pivotal role in mediating cell functions. 55 In addition, the degradations of cytoskeleton proteins such as desmin, talin, and plectin are associated with postmortem muscle quality, contributing to the enhancement of water holding capacity and tenderness of meat. 56 Myofibrillar Proteins.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Postmortem Muscle Proteome Characteristics of Silver Carp (Hypophthalmichthys molitrix): Insights from Full-Length Transcriptome and Deep 4D Label-Free Proteomic

Jiao,

Li,

Zhang

et al. 2024

J. Agric. Food Chem.

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The coverage of the protein database directly determines the results of shotgun proteomics. In this study, PacBio single-molecule real-time sequencing technology was performed on postmortem silver carp muscle transcripts. A total of 42.43 Gb clean data, 35,834 nonredundant transcripts, and 15,413 unigenes were obtained. In total, 99.32% of the unigenes were successfully annotated and assigned specific functions. PacBio long-read isoform sequencing (Iso-Seq) analysis can provide more accurate protein information with a higher proportion of complete coding sequences and longer lengths. Subsequently, 2671 proteins were identified in deep 4D proteomics informed by a full-length transcriptomics technique, which has been shown to improve the identification of low-abundance muscle proteins and potential protein isoforms. The feature of the sarcomeric protein profile and information on more than 30 major proteins in the white dorsal muscle of silver carp were reported here for the first time. Overall, this study provides valuable transcriptome data resources and the comprehensive muscle protein information detected to date for further study into the processing characteristic of early postmortem fish muscle, as well as a spectral library for data-independent acquisition and data processing. This batch of muscle-specific dependent acquisition data is available via PRIDE with identifier PXD043702.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Postmortem Muscle Proteome Characteristics of Silver Carp (Hypophthalmichthys molitrix): Insights from Full-Length Transcriptome and Deep 4D Label-Free Proteomic

Jiao,

Li,

Zhang

et al. 2024

J. Agric. Food Chem.

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“…STK33 absence is associated with asthenozoospermia (31), but other studies suggest a role of STK33 in tumorigenesis (43)(44)(45)(46) and regulation of extracellular signal-regulated kinase (ERK) signaling pathways (47). On the basis of molecular modeling, mouse and human STK33 have identical ATP binding pockets (fig.…”

Section: Validation Of Chemical Perturbation Of Stk33 For Contracepti...mentioning

confidence: 99%

Reversible male contraception by targeted inhibition of serine/threonine kinase 33

Ku,

Sharma,

et al. 2024

Science

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Men or mice with homozygous serine/threonine kinase 33 ( STK33 ) mutations are sterile owing to defective sperm morphology and motility. To chemically evaluate STK33 for male contraception with STK33-specific inhibitors, we screened our multibillion-compound collection of DNA-encoded chemical libraries, uncovered potent STK33-specific inhibitors, determined the STK33 kinase domain structure bound with a truncated hit CDD-2211, and generated an optimized hit CDD-2807 that demonstrates nanomolar cellular potency (half-maximal inhibitory concentration = 9.2 nanomolar) and favorable metabolic stability. In mice, CDD-2807 exhibited no toxicity, efficiently crossed the blood-testis barrier, did not accumulate in brain, and induced a reversible contraceptive effect that phenocopied genetic STK33 perturbations without altering testis size. Thus, STK33 is a chemically validated, nonhormonal contraceptive target, and CDD-2807 is an effective tool compound.

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“…PDCD6 is a multifunctional protein that interacts with microtubules and actin cytoskeleton components (Figure 2b). The cytoskeleton is a highly dynamic network composed of actin filaments, intermediate filaments, and microtubules (Li et al, 2023; Xie et al, 2022). The stability and dynamics of the cytoskeleton are regulated by cytoskeleton‐related proteins and posttranslational modifications (Chen et al, 2021; Sun et al, 2023; Xie et al, 2021; Xie et al, 2022; Yang et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Multifaceted roles of PDCD6 both within and outside the cell

Zhu,

2024

Journal Cellular Physiology

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Programmed cell death protein 6 (PDCD6) is an evolutionarily conserved Ca2+‐binding protein. PDCD6 is involved in regulating multifaceted and pleiotropic cellular processes in different cellular compartments. For instance, nuclear PDCD6 regulates apoptosis and alternative splicing. PDCD6 is required for coat protein complex II‐dependent endoplasmic reticulum‐to‐Golgi apparatus vesicular transport in the cytoplasm. Recent advances suggest that cytoplasmic PDCD6 is involved in the regulation of cytoskeletal dynamics and innate immune responses. Additionally, membranous PDCD6 participates in membrane repair through endosomal sorting complex required for transport complex‐dependent membrane budding. Interestingly, extracellular vesicles are rich in PDCD6. Moreover, abnormal expression of PDCD6 is closely associated with many diseases, especially cancer. PDCD6 is therefore a multifaceted but pivotal protein in vivo. To gain a more comprehensive understanding of PDCD6 functions and to focus and stimulate PDCD6 research, this review summarizes key developments in its role in different subcellular compartments, processes, and pathologies.

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Roles of the cytoskeleton in human diseases

Cited by 6 publications

References 100 publications

Postmortem Muscle Proteome Characteristics of Silver Carp (Hypophthalmichthys molitrix): Insights from Full-Length Transcriptome and Deep 4D Label-Free Proteomic

Postmortem Muscle Proteome Characteristics of Silver Carp (Hypophthalmichthys molitrix): Insights from Full-Length Transcriptome and Deep 4D Label-Free Proteomic

Reversible male contraception by targeted inhibition of serine/threonine kinase 33

Multifaceted roles of PDCD6 both within and outside the cell

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