Roles of Peroxisomes in the Rice Blast Fungus

Chen, Xiao‐Lin; Wang, Zhao; Liu, Caiyun

doi:10.1155/2016/9343417

Cited by 25 publications

(32 citation statements)

References 63 publications

(125 reference statements)

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“…When cells are exposed by UV light and different oxidizing agents, peroxisome proliferation with the formation of tubular peroxisome and up-regulation of peroxins (PEX) related genes will be induced [73]. In M. oryzae , many peroxisome related genes are identified and influence pathogenicity [74]. We found that many up-regulated DEGs involving peroxisomes were enriched both in the WT and ∆ Momcp1 mutant (Table S9).…”

Section: Discussionmentioning

confidence: 98%

MoMCP1, a Cytochrome P450 Gene, Is Required for Alleviating Manganese Toxin Revealed by Transcriptomics Analysis in Magnaporthe oryzae

Wang

Liu

et al. 2019

IJMS

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Manganese, as an essential trace element, participates in many physiological reactions by regulating Mn associated enzymes. Magnaporthe oryzae is a serious pathogen and causes destructive losses for rice production. We identified a cytochrome P450 gene, MoMCP1, involving the alleviation of manganese toxin and pathogenicity. To identify the underlying mechanisms, transcriptomics were performed. The results indicated that many pathogenicity related genes were regulated, especially hydrophobin related genes in ∆Momcp1. Furthermore, the Mn2+ toxicity decreased the expressions of genes involved in the oxidative phosphorylation and energy production, and increased the reactive oxygen species (ROS) levels, which might impair the functions of mitochondrion and vacuole, compromising the pathogenicity and development in ∆Momcp1. Additionally, our results provided further information about Mn associated the gene network for Mn metabolism in cells.

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Section: Discussionmentioning

confidence: 98%

MoMCP1, a Cytochrome P450 Gene, Is Required for Alleviating Manganese Toxin Revealed by Transcriptomics Analysis in Magnaporthe oryzae

Wang

Liu

et al. 2019

IJMS

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“…Up-regulation of large number of PEX genes in our study suggests a possible important role of these genes for S. sclerotiorum pathogenicity/ virulence. Fatty acid β-oxidation is a lipid metabolic pathway for degrading long chain fatty acids for nutrient and energy generation [102,103]. This is an enzyme mediated, four-step process pathway which results in acetyl-CoA, which can be fed into glyoxylate cycle or transported to mitochondria for energy generation through citric acid cycle.…”

Section: Peroxisome Associated Pathwaysmentioning

confidence: 99%

“…The glyoxylate cycle is important for gluconeogenesis, generation of glucose under nutrient scarce condition by assimilating the Acetyl-CoA generated via fatty acid β-oxidation. Isocitrate lyase (ICL1) and Maleate synthetase (MSL1) are two important enzymes of glyoxylate cycle [102]. ICL1 was found to be essential for the pathogenicity of another canola pathogen L. maculans [106]; and was shown to be important for full virulence of M. oryzae [107] and C. orbiculare [108].…”

Section: Peroxisome Associated Pathwaysmentioning

confidence: 99%

Transcriptome analysis of the plant pathogen Sclerotinia sclerotiorum interaction with resistant and susceptible canola (Brassica napus) lines

et al. 2020

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Sclerotinia stem rot is an economically important disease of canola (Brassica napus) and is caused by the fungal pathogen Sclerotinia sclerotiorum. This study evaluated the differential gene expression patterns of S. sclerotiorum during disease development on two canola lines differing in susceptibility to this pathogen. Sequencing of the mRNA libraries derived from inoculated petioles and mycelium grown on liquid medium generated approximately 164 million Illumina reads, including 95 million 75-bp-single reads, and 69 million 50-bp-paired end reads. Overall, 36% of the quality filter-passed reads were mapped to the S. sclerotiorum reference genome. On the susceptible line, 1301 and 1214 S. sclerotiorum genes were differentially expressed at early (8-16 hours post inoculation (hpi)) and late (24-48 hpi) infection stages, respectively, while on the resistant line, 1311 and 1335 genes were differentially expressed at these stages, respectively. Gene ontology (GO) categories associated with cell wall degradation, detoxification of host metabolites, peroxisome related activities like fatty acid ß-oxidation, glyoxylate cycle, oxidoreductase activity were significantly enriched in the up-regulated gene sets on both susceptible and resistant lines. Quantitative RT-PCR of six selected DEGs further validated the RNA-seq differential gene expression analysis. The regulation of effector genes involved in host defense suppression or evasion during the early infection stage, and the expression of effectors involved in host cell death in the late stage of infection provide supporting evidence for a two-phase infection model involving a brief biotrophic phase during early stages of infection. The findings from this study emphasize the role of peroxisome related pathways along with cell wall degradation and detoxification of host metabolites as the key mechanisms underlying pathogenesis of S. sclerotiorum on B. napus.

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“…, Alternaria alternata and Fusarium sp. (Wang et al ., ; Fujihara et al ., ; Imazaki et al ., ; Goh et al ., ; Min et al ., ; Chen et al ., ) although the underlying mechanisms are not well known.…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that the disruption of structural genes of peroxisomes significantly attenuated virulence in several fungal species, including Magnaporthe oryzae, Colletotrichum sp., Alternaria alternata and Fusarium sp. (Wang et al, 2007;Fujihara et al, 2010;Imazaki et al, 2010;Goh et al, 2011;Min et al, 2012;Chen et al, 2016) although the underlying mechanisms are not well known.…”

Section: Introductionmentioning

confidence: 99%

Contribution of peroxisomal docking machinery to mycotoxin biosynthesis, pathogenicity and pexophagy in the plant pathogenic fungus Fusarium graminearum

Chen

Zheng

et al. 2018

Environmental Microbiology

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Peroxisomal proliferation is highly stimulated during the biosynthesis of mycotoxins and plant infection by Fusarium graminearum. Currently, the functions of the peroxisome in these cellular processes are poorly understood. In this study, we applied genetic, cell biological and biochemical analyses to investigate the functions of the peroxisomes. We constructed targeted deletion of docking machinery components, including FgPex13, FgPex14 and the filamentous fungal specific peroxin FgPex33. Our results indicated that peroxisome dysfunction resulted in a shortage of acetyl-CoA, the precursor of trichothecene biosynthesis, and subsequently decreased deoxynivalenol (DON) production. Deletion mutants of ΔFgPex13, ΔFgPex14 or ΔFgPex33 showed an increased accumulation of endogenous reactive oxygen species (ROS) and reduced phosphorylation of MAP (Mitogen-Activated Protein) kinase FgMgv1. In addition, mutants of the docking peroxin exhibited increased sensitivity toward host oxidative bursts and cell wall integrity stress agents and reduced virulence on host plants. More importantly, we found for the first time that FgPex14 is required for pexophagy in F. graminearum. Overall, our study suggests that peroxisomes play critical roles in DON biosynthesis and virulence in F. graminearum.

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Roles of Peroxisomes in the Rice Blast Fungus

Cited by 25 publications

References 63 publications

MoMCP1, a Cytochrome P450 Gene, Is Required for Alleviating Manganese Toxin Revealed by Transcriptomics Analysis in Magnaporthe oryzae

MoMCP1, a Cytochrome P450 Gene, Is Required for Alleviating Manganese Toxin Revealed by Transcriptomics Analysis in Magnaporthe oryzae

Transcriptome analysis of the plant pathogen Sclerotinia sclerotiorum interaction with resistant and susceptible canola (Brassica napus) lines

Contribution of peroxisomal docking machinery to mycotoxin biosynthesis, pathogenicity and pexophagy in the plant pathogenic fungus Fusarium graminearum

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