Roles of PER immunoreactive neurons in circadian rhythms and photoperiodism in the blow fly, <i>Protophormia terraenovae</i>

Shiga, Sakiko; Numata, Hideharu

doi:10.1242/jeb.027003

Cited by 85 publications

(68 citation statements)

References 57 publications

(83 reference statements)

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“…The operation at the control region, where PDF neurons did not reside (Site 2), had no effect on the photoperiodic response, indicating that induction of ovarian development by Site 1 operation was not the result of damage to any brain tissue. Therefore, our result suggests that the region containing PDF neurons is essential for the photoperiodic response, as demonstrated in P. terraenovae (Shiga and Numata, 2009). Removal of this region induced reproduction irrespective of photoperiod, which was expected because disruption of proper functions of the circadian clock under constant light induces ovarian development in R. pedestris (Kobayashi and Numata, 1993).…”

Section: Research Articlesupporting

confidence: 71%

“…These results suggest that the cell bodies of PDFMe neurons, not the fibre patterns, are important for the photoperiodic response. However, there was no correlation between the number of PDFMe cell bodies and reproductive status, as was also observed in P. terraenovae (Shiga and Numata, 2009). These results could be interpreted in several ways.…”

Section: Research Articlementioning

confidence: 49%

“…These results could be interpreted in several ways. The first possibility is that only part of the PDFMe neurons are important for the photoperiodic response and ovarian development induced in shortday conditions was caused by absence of these important neurons, as proposed by Shiga and Numata (Shiga and Numata, 2009). This could be supported by the observation that the PDFMe cluster contains different types of PDF-ir neurons.…”

Section: Research Articlementioning

confidence: 99%

“…Surgical removal of the region containing PDFir s-LN v neurons, which express the circadian clock protein PERIOD, abolished not only the circadian rhythm in locomotor activity but also the photoperiodic regulation of diapause in the blow fly Protophormia terraenovae (Shiga and Numata, 2009). In this insect, s-LN v s were shown to be synaptically connected to neurons with somata in the pars lateralis, called PL neurons, which are indispensable for diapause induction (Hamanaka et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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The involvement of the brain region containing pigment-dispersing factor-immunoreactive neurons in the photoperiodic response of the bean bugRiptortus pedestris

Ikeno

Numata

Goto

et al. 2013

Journal of Experimental Biology

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The concept of insect photoperiodism based on a circadian clock has been supported by many studies demonstrating that the behavioural circadian rhythm and the photoperiodic response are driven by the same circadian clock genes. However, the neuronal mechanism of the circadian clock underlying photoperiodism is poorly understood. To examine whether circadian rhythm and photoperiodism share a neuronal mechanism, we focused on the neurons that express neuropeptide pigment-dispersing factor (PDF) in the bean bug, Riptortus pedestris. PDF has been identified as an important regulator of the insect circadian rhythm and is expressed in circadian clock neurons of various insect species. In R. pedestris, PDF immunoreactivity was detected in some clusters of cells and their fibres in the optic lobe and the protocerebrum. cDNA encoding a PDF precursor protein was highly conserved between R. pedestris and many other insects. Differences between day and night were not observed in the immunolabelling intensity in cell bodies of PDFimmunoreactive neurons and pdf mRNA expression levels in the head. Surgical removal of the region containing PDF-immunoreactive cell bodies at the medulla disrupted the photoperiodic regulation of diapause. However, gene suppression of pdf by RNA interference did not affect the photoperiodic response. These results suggest that the region containing PDF-immunoreactive somata is important for the photoperiodic response in R. pedestris, but pdf mRNA expression is probably not required for the response.

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Section: Research Articlesupporting

confidence: 71%

Section: Research Articlementioning

confidence: 49%

Section: Research Articlementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The involvement of the brain region containing pigment-dispersing factor-immunoreactive neurons in the photoperiodic response of the bean bugRiptortus pedestris

Ikeno

Numata

Goto

et al. 2013

Journal of Experimental Biology

Self Cite

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“…A subset of the core circadian neurons also express pigment dispersing factor (pdf), a circadian clock-associated gene that maintains synchronous cycling among individual circadian clock neurons (Shafer and Yao, 2014). These PDF-positive neurons innervate regions of the brain involved in the hormonal regulation of insect diapause in the blow fly Protophormia terraenovae, and ablation of these PDF-positive neurons interferes with photoperiodic diapause induction (Hamanaka et al, 2005;Shiga and Numata, 2009). Such studies suggest a physical connection between the circadian and photoperiodic clocks, as well as the potential for PDF to coordinate both daily and seasonal responses.…”

Section: Introductionmentioning

confidence: 99%

Functional circadian clock genes are essential for the overwintering diapause of the Northern house mosquito,Culex pipiens

Meuti

Stone

Ikeno

et al. 2015

Journal of Experimental Biology

137

101

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The short day lengths of late summer are used to program the overwintering adult diapause (dormancy) of the Northern house mosquito, Culex pipiens. Here, we investigated the role of clock genes in initiating this diapause and asked whether the circadian cycling of clock gene expression persists during diapause. We provide evidence that the major circadian clock genes continue to cycle throughout diapause and after diapause has been terminated. RNA interference (RNAi) was used to knock down the core circadian clock genes and to then assess the impact of the various clock genes on the ability of females to enter diapause. RNAi directed against negative circadian regulators (period, timeless and cryptochrome2) caused females that were reared under diapause-inducing, short day conditions to avert diapause. In contrast, knocking down the circadian-associated gene pigment dispersing factor caused females that were reared under diapause-averting, long day conditions to enter a diapause-like state. Our results implicate the circadian clock in the initiation of diapause in C. pipiens.

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The circadian clock network in the brain of different Drosophila species

Hermann

Saccon

Senthilan

et al. 2012

J of Comparative Neurology

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Comparative studies on cellular and molecular clock mechanisms have revealed striking similarities in the organization of the clocks among different animal groups. To gain evolutionary insight into the properties of the clock network within the Drosophila genus, we analyzed sequence identities and similarities of clock protein homologues and immunostained brains of 10 different Drosophila species using antibodies against vrille (VRI), PAR-protein domain1 (PDP1), and cryptochrome (CRY). We found that the clock network of both subgenera Sophophora and Drosophila consists of all lateral and dorsal clock neuron clusters that were previously described in Drosophila melanogaster. Immunostaining against CRY and the neuropeptide pigment-dispersing factor (PDF), however, revealed species-specific differences. All species of the Drosophila subgenus and D. pseudoobscura of the Sophophora subgenus completely lacked CRY in the large ventrolateral clock neurons (lLN(v) s) and showed reduced PDF immunostaining in the small ventrolateral clock neurons (sLN(v) s). In contrast, we found the expression of the ion transport peptide (ITP) to be consistent within the fifth sLN(v) and one dorsolateral clock neuron (LN(d) ) in all investigated species, suggesting a conserved putative function of this neuropeptide in the clock. We conclude that the general anatomy of the clock network is highly conserved throughout the Drosophila genus, although there is variation in PDF and CRY expression. Our comparative study is a first step toward understanding the organization of the circadian clock in Drosophila species adapted to different habitats.

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Roles of PER immunoreactive neurons in circadian rhythms and photoperiodism in the blow fly, Protophormia terraenovae

Cited by 85 publications

References 57 publications

The involvement of the brain region containing pigment-dispersing factor-immunoreactive neurons in the photoperiodic response of the bean bugRiptortus pedestris

The involvement of the brain region containing pigment-dispersing factor-immunoreactive neurons in the photoperiodic response of the bean bugRiptortus pedestris

Functional circadian clock genes are essential for the overwintering diapause of the Northern house mosquito,Culex pipiens

The circadian clock network in the brain of different Drosophila species

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