Roles of ompA of Citrobacter werkmanii in bacterial growth, biocide resistance, biofilm formation and swimming motility

Zhou, Gang; Wang, Ying-si; Peng, Hong; Li, Sujuan; Sun, Tairen; Shen, Pengfei; Xie, Xiaobao; Shi, Qingshan

doi:10.1007/s00253-020-11057-1

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…The protein encoded by omp A can bind to the receptor on the surface of the host cell so that bacteria can adhere to the host cell closely, thus achieving bacterial invasion [ 39 ]. In addition, omp A could enhance the stability of the bacterial outer membrane and help bacteria resist the invasion of environmental factors such as external mechanical pressure [ 40 ]. When bacteria are faced with oxidative stress, reactive oxygen species cause damage to proteins, DNA and other molecules in cells.…”

Section: Resultsmentioning

confidence: 99%

The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

Yan,

Chen,

Yang

et al. 2023

IJMS

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The high cell density, immobilization and stability of biofilms are ideal characteristics for bacteria in resisting antibiotic therapy. CsgD is a transcription activating factor that regulates the synthesis of curly fimbriae and cellulose in Escherichia coli, thereby enhancing bacterial adhesion and promoting biofilm formation. To investigate the role of CsgD in biofilm formation and stress resistance in bacteria, the csgD deletion mutant ΔcsgD was successfully constructed from the engineered strain E. coli BL21(DE3) using the CRISPR/Cas9 gene-editing system. The results demonstrated that the biofilm of ΔcsgD decreased by 70.07% (p < 0.05). Additionally, the mobility and adhesion of ΔcsgD were inhibited due to the decrease in curly fimbriae and extracellular polymeric substances. Furthermore, ΔcsgD exhibited a significantly decreased resistance to acid, alkali and osmotic stress conditions (p < 0.05). RNA-Seq results revealed 491 differentially expressed genes between the parent strain and ΔcsgD, with enrichment primarily observed in metabolism-related processes as well as cell membrane structure and catalytic activity categories. Moreover, CsgD influenced the expression of biofilm and stress response genes pgaA, motB, fimA, fimC, iraP, ompA, osmC, sufE and elaB, indicating that the CsgD participated in the resistance of E. coli by regulating the expression of biofilm and stress response. In brief, the transcription factor CsgD plays a key role in the stress resistance of E. coli, and is a potential target for treating and controlling biofilm.

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Section: Resultsmentioning

confidence: 99%

The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

Yan,

Chen,

Yang

et al. 2023

IJMS

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“…In particular, curli has been well characterized in pathogenic E. coli and S. enterica and has a role in biofilm formation and in attachment to the extracellular matrix and colonization of the mammalian host cells [ 74 ]. Moreover, recent studies on the C. werkmanii strain BF-6 have demonstrated that OmpA plays an important role in the regulation of multiple virulence phenotypes, including biofilm formation on distinct surfaces, swimming motility, metal ion responses, and resistance to biocides [ 75 ]. Finally, the virulence typing analyses of C. werkmanii indicated the presence of the siderophore enterobactin genes for the acquisition of iron, an essential nutrient for bacteria growth and the increased virulence potential of Citrobacter [ 76 , 77 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomic Analyses of Virulence and Antimicrobial Resistance in Citrobacter werkmanii, an Emerging Opportunistic Pathogen

Aguirre-Sánchez,

Quiñones,

Ortiz-Muñoz

et al. 2023

Microorganisms

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Citrobacter werkmanii is an emerging and opportunistic human pathogen found in developing countries and is a causative agent of wound, urinary tract, and blood infections. The present study conducted comparative genomic analyses of a C. werkmanii strain collection from diverse geographical locations and sources to identify the relevant virulence and antimicrobial resistance genes. Pangenome analyses divided the examined C. werkmanii strains into five distinct clades; the subsequent classification identified genes with functional roles in carbohydrate and general metabolism for the core genome and genes with a role in secretion, adherence, and the mobilome for the shell and cloud genomes. A maximum-likelihood phylogenetic tree with a heatmap, showing the virulence and antimicrobial genes’ presence or absence, demonstrated the presence of genes with functional roles in secretion systems, adherence, enterobactin, and siderophore among the strains belonging to the different clades. C. werkmanii strains in clade V, predominantly from clinical sources, harbored genes implicated in type II and type Vb secretion systems as well as multidrug resistance to aminoglycoside, beta-lactamase, fluoroquinolone, phenicol, trimethoprim, macrolides, sulfonamide, and tetracycline. In summary, these comparative genomic analyses have demonstrated highly pathogenic and multidrug-resistant genetic profiles in C. werkmanii strains, indicating a virulence potential for this commensal and opportunistic human pathogen.

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“…The ompA mutant of E. coli was more sensitive to a variety of antibiotics, including β-lactams, amphenicols, glycopeptides, and lincosamides, compared to the WT [ 6 ]. Furthermore, our team discovered that Citrobacter werkmanii showed increased 1,2-benzisothiazolin-3-one (BIT) resistance following the inactivation of ompA [ 37 ]. By preserving the stability of the outer membrane, OmpA also defends Salmonella Typhimurium from the two β-lactam antibiotics: ceftazidime and meropenem [ 38 ].…”

Section: Ompamentioning

confidence: 99%

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

Zhou

Wang

et al. 2023

Microorganisms

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Gram-negative bacteria depend on their cell membranes for survival and environmental adaptation. They contain two membranes, one of which is the outer membrane (OM), which is home to several different outer membrane proteins (Omps). One class of important Omps is porins, which mediate the inflow of nutrients and several antimicrobial drugs. The microorganism’s sensitivity to antibiotics, which are predominantly targeted at internal sites, is greatly influenced by the permeability characteristics of porins. In this review, the properties and interactions of five common porins, OmpA, OmpC, OmpF, OmpW, and OmpX, in connection to porin-mediated permeability are outlined. Meanwhile, this review also highlighted the discovered regulatory characteristics and identified molecular mechanisms in antibiotic penetration through porins. Taken together, uncovering porins’ functional properties will pave the way to investigate effective agents or approaches that use porins as targets to get rid of resistant gram-negative bacteria.

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Roles of ompA of Citrobacter werkmanii in bacterial growth, biocide resistance, biofilm formation and swimming motility

Cited by 7 publications

References 43 publications

The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

Comparative Genomic Analyses of Virulence and Antimicrobial Resistance in Citrobacter werkmanii, an Emerging Opportunistic Pathogen

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

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