Roles of NOLC1 in cancers and viral infection

Zhai, Fengguang; Wang, Jie; Meng, Yahan; Jin, Xiaofeng

doi:10.1007/s00432-023-04934-5

Cited by 4 publications

(3 citation statements)

References 146 publications

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“…The nucleolar and coiled-body phosphoprotein 1 ( NOLC1 ) protein is responsible for various cellular life activities, including ribosome biosynthesis, DNA replication, transcription regulation, RNA processing, cell cycle regulation, apoptosis and cell regeneration [ 46 ]. Our results demonstrated that gene expression was higher in samples of gastric cancer tumors than in normal tissues ( Figure 2 ), which was confirmed by RT-qPCR for the GC cell lines ACP-03 and AGS ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

A Shortcut from Genome to Drug: The Employment of Bioinformatic Tools to Find New Targets for Gastric Cancer Treatment

Brito,

Lima,

Mesquita

et al. 2023

Pharmaceutics

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Gastric cancer (GC) is a highly heterogeneous, complex disease and the fifth most common cancer worldwide (about 1 million cases and 784,000 deaths worldwide in 2018). GC has a poor prognosis (the 5-year survival rate is less than 20%), but there is an effort to find genes highly expressed during tumor establishment and use the related proteins as targets to find new anticancer molecules. Data were collected from the Gene Expression Omnibus (GEO) bank to obtain three dataset matrices analyzing gastric tumor tissue versus normal gastric tissue and involving microarray analysis performed using the GPL570 platform and different sources. The data were analyzed using the GEPIA tool for differential expression and KMPlot for survival analysis. For more robustness, GC data from the TCGA database were used to corroborate the analysis of data from GEO. The genes found in in silico analysis in both GEO and TCGA were confirmed in several lines of GC cells by RT-qPCR. The AlphaFold Protein Structure Database was used to find the corresponding proteins. Then, a structure-based virtual screening was performed to find molecules, and docking analysis was performed using the DockThor server. Our in silico and RT-qPCR analysis results confirmed the high expression of the AJUBA, CD80 and NOLC1 genes in GC lines. Thus, the corresponding proteins were used in SBVS analysis. There were three molecules, one molecule for each target, MCULE-2386589557-0-6, MCULE-9178344200-0-1 and MCULE-5881513100-0-29. All molecules had favorable pharmacokinetic, pharmacodynamic and toxicological properties. Molecular docking analysis revealed that the molecules interact with proteins in critical sites for their activity. Using a virtual screening approach, a molecular docking study was performed for proteins encoded by genes that play important roles in cellular functions for carcinogenesis. Combining a systematic collection of public microarray data with a comparative meta-profiling, RT-qPCR, SBVS and molecular docking analysis provided a suitable approach for finding genes involved in GC and working with the corresponding proteins to search for new molecules with anticancer properties.

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Section: Discussionmentioning

confidence: 99%

A Shortcut from Genome to Drug: The Employment of Bioinformatic Tools to Find New Targets for Gastric Cancer Treatment

Brito,

Lima,

Mesquita

et al. 2023

Pharmaceutics

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“…Another link between CB and phosphorylation is the protein NOLC1/Nopp140, which is one of the most phosphorylated proteins in the cell. NOLC1 is an essential protein that accumulates in nucleoli and CBs (reviewed in [ 85 ]. NOLC1 interacts with small nucleolar RNPs (snoRNPs) but is not directly involved in rRNA modifications and has been suggested to act as an snoRNP chaperone [ 86–88 ].…”

Section: Cajal Body Formationmentioning

confidence: 99%

Coilin and Cajal bodies

Staněk

2023

Nucleus

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The nucleus of higher eukaryotes contains a number of structures that concentrate specific biomolecules and play distinct roles in nuclear metabolism. In recent years, the molecular mechanisms controlling their formation have been intensively studied. In this brief review, I focus on coilin and Cajal bodies. Coilin is a key scaffolding protein of Cajal bodies that is evolutionarily conserved in metazoans. Cajal bodies are thought to be one of the archetypal nuclear structures involved in the metabolism of several short non-coding nuclear RNAs. Yet surprisingly little is known about the structure and function of coilin, and a comprehensive model to explain the origin of Cajal bodies is also lacking. Here, I summarize recent results on Cajal bodies and coilin and discuss them in the context of the last three decades of research in this field.

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“…Indeed, recent studies have shown that FGF11-14 interact with several nuclear proteins, including the nucleolar and coiled-body phosphoprotein 1, NOLC1 (Nopp140) and treacle ribosome biogenesis factor 1, TCOF1 (Treacle) [ 6 , 21 ]. NOLC1 is a natively unfolded scaffold protein that forms a complex with TCOF1, which in turn connects RNA polymerase I with ribosome-modifying enzymes to facilitate ribosome biogenesis [ 22 – 24 ]. Notably, TCOF1 mutations are behind the ribosomopathy observed as Treachers-Collins Syndrome [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

The intracellular interplay between galectin-1 and FGF12 in the assembly of ribosome biogenesis complex

Gędaj,

Chorążewska,

Ciura

et al. 2024

Cell Commun Signal

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Galectins constitute a class of lectins that specifically interact with β-galactoside sugars in glycoconjugates and are implicated in diverse cellular processes, including transport, autophagy or signaling. Since most of the activity of galectins depends on their ability to bind sugar chains, galectins exert their functions mainly in the extracellular space or at the cell surface, which are microenvironments highly enriched in glycoconjugates. Galectins are also abundant inside cells, but their specific intracellular functions are largely unknown. Here we report that galectin-1, -3, -7 and -8 directly interact with the proteinaceous core of fibroblast growth factor 12 (FGF12) in the cytosol and in nucleus. We demonstrate that binding of galectin-1 to FGF12 in the cytosol blocks FGF12 secretion. Furthermore, we show that intracellular galectin-1 affects the assembly of FGF12-containing nuclear/nucleolar ribosome biogenesis complexes consisting of NOLC1 and TCOF1. Our data provide a new link between galectins and FGF proteins, revealing an unexpected glycosylation-independent intracellular interplay between these groups of proteins.

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Roles of NOLC1 in cancers and viral infection

Cited by 4 publications

References 146 publications

A Shortcut from Genome to Drug: The Employment of Bioinformatic Tools to Find New Targets for Gastric Cancer Treatment

A Shortcut from Genome to Drug: The Employment of Bioinformatic Tools to Find New Targets for Gastric Cancer Treatment

Coilin and Cajal bodies

The intracellular interplay between galectin-1 and FGF12 in the assembly of ribosome biogenesis complex

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