Roles of Mso1 and the SM protein Sec1 in efficient vesicle fusion during fission yeast cytokinesis

Gerien, Kenneth S.; Zhang, Sha; Russell, Alexandra; Zhu, Yihua; Purde, Vedud; Wu, Jian-Qiu

doi:10.1091/mbc.e20-01-0067

Cited by 3 publications

(3 citation statements)

References 82 publications

(105 reference statements)

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“…The secondary structure analysis of the VPS33B protein in this patient shows that p .Q361X,257 and p .C82R are indeed located in the Sec1 protein domain ( Figure 3A ). Sec-1/Munc18 protein (SM protein) regulates vesicle trafficking and fusion by binding to the T-SNARE of the Syntaxin family in a variety of modes, and SM protein plays an important role in membrane protein trafficking and maintenance of cell polarity ( 5 , 6 ). This protein binds and modulates the conformation of SNARE protein, which is homologous to neuronal synaptic membrane protein syntaxin and is critical for neurotransmitter release and other secretory events ( 7 ).…”

Section: Discussionmentioning

confidence: 99%

Two novel mutations in VPS33B gene cause a milder ARC syndrome with prolonged survival in a 12-year-old patient: Case report

Zhu

Chen

2022

Front. Pediatr.

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Arthrogryposis–renal dysfunction–cholestasis (ARC) syndrome is a rare autosomal recessive disease caused by VPS33B and VIPAR gene mutations. The main clinical manifestations are congenital joint contracture, renal dysfunction mainly characterized by distal renal tubular dysfunction, and low glutamyltransferase cholestasis. Most patients with ARC die within 2 years of birth. Here, we report the case of a 12-year-old girl with an ARC phenotype who experienced long-term survival with only mild clinical symptoms. We detected two new heterozygous mutation sites of the VPS33B gene in this child, c.1081C > T (p.GLN361X, 257) and c.244T > C (p.Cys82Arg), through the gene detection technique; the tertiary structure of the protein was predicted by using the SWISS-model. We further reviewed the literature and summarized the clinical manifestations and gene loci of 19 ARC syndrome patients with long-term survival reported so far.

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Section: Discussionmentioning

confidence: 99%

Two novel mutations in VPS33B gene cause a milder ARC syndrome with prolonged survival in a 12-year-old patient: Case report

Zhu

Chen

2022

Front. Pediatr.

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“…We carried out coimmunoprecipitation and Western blotting similarly to previously described methods ( Cortes et al. , 2012 ; Lee and Wu, 2012 ; Gerien et al. , 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…We carried out co-immunoprecipitation and Western blotting similarly to previously described (Cortes et al, 2012;Lee and Wu, 2012;Gerien et al, 2020). Lyophilized cells (200 mg) of each strain were ground into fine powder.…”

Section: Co-immunoprecipitation and Western Blottingmentioning

confidence: 99%

Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis

Longo

Goodyear

Zhang

et al. 2022

MBoC

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Cytokinesis is the final step of the cell-division cycle. In fungi, it relies on the coordination of constriction of an actomyosin contractile ring and construction of the septum at the division site. Glucan synthases synthesize glucans, which are the major components in fungal cell walls and division septa. It is known that Rho1 and Rho2 GTPases regulate glucan synthases Bgs1, Bgs4, and Ags1, and Sbg1 and the F-BAR protein Cdc15 play roles in Bgs1 stability and delivery to the plasma membrane. Here we characterize Smi1, an intrinsically disordered protein that interacts with Bgs4 and regulates its trafficking and localization in fission yeast. Smi1 is important for septum integrity, and its absence causes severe lysis during cytokinesis. Smi1 localizes to secretory vesicles and moves together with Bgs4 towards the division site. The concentrations of the glucan synthases Bgs1 and Bgs4 and the glucanases Agn1 and Bgl2 decrease at the division site in smi1 mutant, but Smi1 seems to be more specific to Bgs4. Mistargeting of Smi1 to mitochondria mislocalizes Bgs4, but not Bgs1. Together, our data reveal a novel regulator of glucan synthases and glucanases, Smi1, which is more important for Bgs4 trafficking, stability, and localization during cytokinesis. [Media: see text] [Media: see text]

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Lipid Polarization during Cytokinesis

Govind

Acharya

2022

Cells

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The plasma membrane of eukaryotic cells is composed of a large number of lipid species that are laterally segregated into functional domains as well as asymmetrically distributed between the outer and inner leaflets. Additionally, the spatial distribution and organization of these lipids dramatically change in response to various cellular states, such as cell division, differentiation, and apoptosis. Division of one cell into two daughter cells is one of the most fundamental requirements for the sustenance of growth in all living organisms. The successful completion of cytokinesis, the final stage of cell division, is critically dependent on the spatial distribution and organization of specific lipids. In this review, we discuss the properties of various lipid species associated with cytokinesis and the mechanisms involved in their polarization, including forward trafficking, endocytic recycling, local synthesis, and cortical flow models. The differences in lipid species requirements and distribution in mitotic vs. male meiotic cells will be discussed. We will concentrate on sphingolipids and phosphatidylinositols because their transbilayer organization and movement may be linked via the cytoskeleton and thus critically regulate various steps of cytokinesis.

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Roles of Mso1 and the SM protein Sec1 in efficient vesicle fusion during fission yeast cytokinesis

Abstract: Mso1 and the SM protein Sec1 aid in the efficient fusion of vesicles at the division site in fission yeast, which is important for proper contractile-ring constriction and plasma-membrane closure during cytokinesis.

Cited by 3 publications

References 82 publications

Two novel mutations in VPS33B gene cause a milder ARC syndrome with prolonged survival in a 12-year-old patient: Case report

Two novel mutations in VPS33B gene cause a milder ARC syndrome with prolonged survival in a 12-year-old patient: Case report

Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis

Lipid Polarization during Cytokinesis

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