Roles of magnesium and calcium ions in cell-to-substrate adhesion

Takeichi, Masatoshi; Okada, Tamotsu

doi:10.1016/0014-4827(72)90480-6

Cited by 130 publications

(85 citation statements)

References 20 publications

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“…According to these assays, Ca and Mg significantly enhanced the adhesive properties of epithelial cells and fibroblasts, consistent with previous reports in which divalent cations promote the initial attachment of epithelial and fibroblastic cells to a negatively charged plastic dish [32,33]. These treatments also enhanced adhesive strength in fibroblasts, although they were unable to act similarly in epithelial cells.…”

Section: Discussionsupporting

confidence: 90%

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

et al. 2012

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In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium plates hydrothermally-treated with CaCl2 (Ca) or MgCl2 (Mg) solution, or distilled water (DW). The number and adhesive strengths of attached cells on the substrata were then analyzed. The number of Sa3 cells adhering to the Ca- and Mg-treated plates was significantly larger than in the DW group, but the strength of this adhesion did not differ significantly between groups. In contrast, NIH3T3 cell adhesion number and strength were increased in both the Ca and Mg groups compared to the DW group. Fluorescent microscopic observation indicated that, in all groups, Sa3 had identical expression levels of integrin β4 and development of actin filaments, whereas NIH3T3 cells in the Ca and Mg groups displayed much stronger punctate cytoplasmic signals for vinculin and more bundle-shaped actin filaments than cells in the DW group. As a result, it was indicated that the hydrothermal treatment of titanium with Ca or Mg solution improved the integration of soft tissue cells with the substrata, which may facilitate the development of a soft tissue barrier around the implant.

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Section: Discussionsupporting

confidence: 90%

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

et al. 2012

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“…It has been indicated that migrating neural crest cells interact closely with surrounding tissues in their pathways (WESTON, 1970(WESTON, , 1972JOHNSTON and LISTGARTEN, 1972;NODEN, 1975). Furthermore, by in vitro experiments of cell adhesion to the substrate such as the adhesion of embryonic fibroblasts to glass or plastics, it was made clear that cells adhere to the substrate by protruding processes (CURTIS, 1964;TAKEICHI and OKADA, 1972). LESSEPS (1963) observed in embryonic heart cells and pigmented retinal cells that cell contact was actually initiated at the crests of their undulating surfaces.…”

Section: Discussionmentioning

confidence: 99%

Electron microscope study on the contact of neural crest cells in the early stage of migration in bantam embryos.

Takahashi

Yamadori

1979

Arch. Histol. Jap., Arch. Histol. Jpn

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“…and Mg 2? ions from the cell monolayer, since these two ions have a role in increasing cell to substrate adhesion in animal cell cultures (Takeichi and Okada 1972). In preliminary experiments, cultures that were treated with TrypLE TM Express were microscopically observed at 5 min intervals to gauge the time it would take for the complete dissociation of the monolayer.…”

Section: Development Of a Technique To Count Dsir-ha-1179 Cellsmentioning

confidence: 99%

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

et al. 2013

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The DSIR-HA-1179 coleopteran cell line is a susceptible and permissive host to the Oryctes rhinoceros nudivirus (OrNV), which has been used as a biocontrol agent against the coconut rhinoceros beetle (Oryctes rhinoceros); a pest of palms in the Asia-Pacific region. However, little is known about growth and metabolism of this cell line, knowledge of which is necessary to develop an in vitro large-scale OrNV production process. The strong anchoragedependent characteristics of the cell line, its particular fragility and its tendency to form dense clumps when manipulated, are the most likely reasons that have precluded further development of the cell line. In order to characterize DSIR-HA-1179 cells, there was first a need for a reliable technique to count the cells. A homogenous cell suspension suitable for enumeration could be produced by treatment with TrypLE Express TM with optimum mean time for cell release calculated as 30 min. The cell line was adapted to grow in four serum-supplemented culture media namely TC-100, IPL-41, Sf-900 II and Sf-900 III and cell growth, glucose consumption, lactate and ammonia production were assessed from static-batch cultures. The maximum viable cell density was reached in Sf-900 II (17.9 9 10 5 cells/ml), with the maximum specific growth rate observed in this culture medium as well (0.0074 h -1 ). Higher production of OrNV was observed in IPL-41 and TC-100 (4.1 9 10 7 TCID 50 /ml) than in cultures infected in Sf-900 III (2.0 9 10 7 TCID 50 /ml) and Sf-900 II (1.4 9 10 7 TCID 50 /ml). At the end of the growth period, glucose was completely consumed in cultures grown in TC-100, while remained in excess in the other three culture media. The cell line produced lactate and ammonia to very low levels in the TC-100 culture medium which is a promising aspect for its cultivation at large-scale.

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Roles of magnesium and calcium ions in cell-to-substrate adhesion

Cited by 130 publications

References 20 publications

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

Electron microscope study on the contact of neural crest cells in the early stage of migration in bantam embryos.

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

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