Roles of Kinases in Osteoblast Function

Matsuguchi, Tetsuya

doi:10.5772/38384

Cited by 1 publication

(1 citation statement)

References 104 publications

(109 reference statements)

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“…The role of kinases in osteoblast differentiation was recently reviewed [38]. In addition to their role in proliferation and apoptosis, MAPKs were found to be key players in the mechanism of osteoblast differentiation.…”

Section: Introductionmentioning

confidence: 99%

Combinatorial MAPLE gradient thin film assemblies signalling to human osteoblasts

Axente

Sima

et al. 2014

Biofabrication

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There is increased interest in smart bioactive materials to control tissue regeneration for the engineering of cell instructive scaffolds. We introduced combinatorial matrix-assisted pulsed laser evaporation (C-MAPLE) as a new method for the fabrication of organic thin films with a compositional gradient. Synchronized C-MAPLE of levan and oxidized levan was employed to assemble a two-compound biopolymer film structure. The gradient of the film composition was validated by fluorescence microscopy. In this study, we investigated the cell response induced by the compositional gradient using imaging of early osteoblast attachment and analysis of signalling phosphoprotein expression. Cells attached along the gradient in direct proportion to oxidized levan concentration. During this process distinct areas of the binary gradient have been shown to modulate the osteoblasts' extracellular signal-regulated kinase signalling with different propensity. The proposed fabrication method results in the preparation of a new bioactive material, which could control the cell signalling response. This approach can be extended to screen new bioactive interfaces for tissue regeneration.

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Section: Introductionmentioning

confidence: 99%