Roles of ionic strength and biofilm roughness on adhesion kinetics of Escherichia coli onto groundwater biofilm grown on PVC surfaces

Janjaroen, Dao; Ling, Fangqiong; Monroy, Guillermo L.; Derlon, Nicolas; Mogenroth, Eberhard; Boppart, Stephen A.; Liu, Wen Tso; Nguyen, Thanh H.

doi:10.1016/j.watres.2013.02.032

Cited by 96 publications

(67 citation statements)

References 60 publications

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“…Prior to use, water was passed through a greensand filter to remove iron and manganese, which could precipitate and change the surface characteristics of the BSF. Newmark groundwater, characterized in previous studies, was found to contain 1−2 mg/L TOC 16 and turbidity between 0.25 and 0.7 NTU. 10 Newmark groundwater has also been shown to form biofilms in previous studies.…”

Section: ■ Materials and Methodsmentioning

confidence: 78%

“…10 Newmark groundwater has also been shown to form biofilms in previous studies. 16,17 In addition, the sand media and groundwater were analyzed for total dissolved Al, Ca, Fe, Mg, and Zn for the sand media and Fe, S, and Cl for the groundwater using inductively coupled plasma-optical emission spectrometry (ICP-OES). Daily influent and effluent samples from the filter were analyzed for pH, dissolved oxygen concentration, and alkalinity.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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MS2 Bacteriophage Reduction and Microbial Communities in Biosand Filters

Wang

Narihiro

Straub

et al. 2014

Environ. Sci. Technol.

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This study evaluated the role of physical and biological filter characteristics on the reduction of MS2 bacteriophage in biosand filters (BSFs). Three full-scale concrete Version 10 BSFs, each with a 55 cm sand media depth and a 12 L charge volume, reached 4 log10 reduction of MS2 within 43 days of operation. A consistently high reduction of MS2 between 4 log10 and 7 log10 was demonstrated for up to 294 days. Further examining one of the filters revealed that an average of 2.8 log10 reduction of MS2 was achieved within the first 5 cm of the filter, and cumulative virus reduction reached an average of 5.6 log10 after 240 days. Core sand samples from this filter were taken for protein, carbohydrate, and genomic extraction. Higher reduction of MS2 in the top 5 cm of the sand media (0.56 log10 reduction per cm vs 0.06 log10 reduction per cm for the rest of the filter depth) coincided with greater diversity of microbial communities and increased concentrations of carbohydrates. In the upper layers, "Candidatus Nitrosopumilus maritimus" and "Ca. Nitrospira defluvii" were found as dominant populations, while significant amounts of Thiobacillus-related OTUs were detected in the lower layers. Proteolytic bacterial populations such as the classes Sphingobacteria and Clostridia were observed over the entire filter depth. Thus, this study provides the first insight into microbial community structures that may play a role in MS2 reduction in BSF ecosystems. Overall, besides media ripening and physical reduction mechanisms such as filter depth and long residence time (45 min vs 24 ± 8.5 h), the establishment of chemolithotrophs and proteolytic bacteria could greatly enhance the reduction of MS2.

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Section: ■ Materials and Methodsmentioning

confidence: 78%

Section: ■ Materials and Methodsmentioning

confidence: 99%

MS2 Bacteriophage Reduction and Microbial Communities in Biosand Filters

Wang

Narihiro

Straub

et al. 2014

Environ. Sci. Technol.

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“…The Hamaker constant used to evaluate the van der Waals interactions between chitin, water, and X. fastidiosa was A 132 of 3 ϫ 10 Ϫ21 J. This constant (A 132 ) was calculated using the geometric mean (25) of known constants for water (25) and bacteria (26), and the Hamaker constant was determined using the contact angle of the BGSS hindwings (27,28). Using this information, interaction profiles were developed by assuming sphere-plate geometry (23)(24)(25)29).…”

Section: Methodsmentioning

confidence: 99%

O Antigen Modulates Insect Vector Acquisition of the Bacterial Plant Pathogen Xylella fastidiosa

Rapicavoli

Kinsinger

Perring

et al. 2015

Appl Environ Microbiol

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e Hemipteran insect vectors transmit the majority of plant pathogens. Acquisition of pathogenic bacteria by these piercing/sucking insects requires intimate associations between the bacterial cells and insect surfaces. Lipopolysaccharide (LPS) is the predominant macromolecule displayed on the cell surface of Gram-negative bacteria and thus mediates bacterial interactions with the environment and potential hosts. We hypothesized that bacterial cell surface properties mediated by LPS would be important in modulating vector-pathogen interactions required for acquisition of the bacterial plant pathogen Xylella fastidiosa, the causative agent of Pierce's disease of grapevines. Utilizing a mutant that produces truncated O antigen (the terminal portion of the LPS molecule), we present results that link this LPS structural alteration to a significant decrease in the attachment of X. fastidiosa to blue-green sharpshooter foreguts. Scanning electron microscopy confirmed that this defect in initial attachment compromised subsequent biofilm formation within vector foreguts, thus impairing pathogen acquisition. We also establish a relationship between O antigen truncation and significant changes in the physiochemical properties of the cell, which in turn affect the dynamics of X. fastidiosa adhesion to the vector foregut. Lastly, we couple measurements of the physiochemical properties of the cell with hydrodynamic fluid shear rates to produce a Comsol model that predicts primary areas of bacterial colonization within blue-green sharpshooter foreguts, and we present experimental data that support the model. These results demonstrate that, in addition to reported protein adhesin-ligand interactions, O antigen is crucial for vector-pathogen interactions, specifically in the acquisition of this destructive agricultural pathogen. Insect vectors transmit numerous pathogens to a wide range of animal and plant hosts. Hemipteran vectors, such as aphids, leafhoppers, and whiteflies, are an economically important group of insects because they are the dominant vectors of plant pathogens (1). The molecular determinants of transmission have been explored for only a few phytopathosystems (compared to mammalian systems), with virus-vector interactions being the most extensively studied. Surface entities, such as virion capsid components, have been shown to be important for the retention and transmission of plant viruses (2), indicating the importance of pathogen surface properties in mediating these interactions. However, we have much less information regarding the molecular mechanisms of insect transmission of bacterial pathogens, specifically those infecting plants.Xylella fastidiosa is a Gram-negative bacterium that causes diseases in several economically important crops, including Pierce's disease (PD) of grapevines, citrus variegated chlorosis, and almond leaf scorch. X. fastidiosa forms biofilms within the xylem vessels of plant hosts, which occludes vessels and impedes water flow within the vine (3). Symptoms of PD include marginal l...

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“…[41] Recently, Janjaroen et al monitored the attachment of Escherichia coli onto clean PVC surfaces. [42] They observed that both cell adhesion and biofilm roughness increased with ionic strength (3-10 mm KCl), and suggested that the physical structure of biofilms could facilitate the adhesion of E. coli cells.…”

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confidence: 99%

Correlation of the Electrochemical Kinetics of High‐Salinity‐Tolerant Bioanodes with the Structure and Microbial Composition of the Biofilm

et al. 2014

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Increasing the conductivity of the electrolytes used in microbial electrochemical systems is an essential prerequisite to large‐scale application of these technologies. Microbial anodes formed on carbon felt from a salt marsh inoculum under polarisation at 0.1 V (versus a saturated calomel electrode), generated up to 85 A m−2 in media that contained 30–45 g L−1 of NaCl. Analyses of microbial populations showed a stringent selection of the two microbial genera Marinobacter and Desulfuromonas. Currents decreased if NaCl concentration was increased to 60 g L−1. This highest salinity was shown to consistently impact the bioanode performance in three ways: voltammetry indicated degraded electron‐transfer kinetics, confocal laser scanning microscopy showed a modified biofilm structure and DNA pyrosequencing detected a decrease in the level of Desulfuromonas spp. relative to Marinobacter spp. A consistent correlation was, thus, found between electrochemical kinetics, biofilm structure and the composition of the microbial community.

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Roles of ionic strength and biofilm roughness on adhesion kinetics of Escherichia coli onto groundwater biofilm grown on PVC surfaces

Cited by 96 publications

References 60 publications

MS2 Bacteriophage Reduction and Microbial Communities in Biosand Filters

MS2 Bacteriophage Reduction and Microbial Communities in Biosand Filters

O Antigen Modulates Insect Vector Acquisition of the Bacterial Plant Pathogen Xylella fastidiosa

Correlation of the Electrochemical Kinetics of High‐Salinity‐Tolerant Bioanodes with the Structure and Microbial Composition of the Biofilm

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