Roles of Ion Channels in Initiation of Signal Transduction in Higher Plants.

Ward, John M.; Pei, Zhen-Ming; Schroeder, Julian

doi:10.1105/tpc.7.7.833

Cited by 261 publications

(85 citation statements)

References 68 publications

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“…Furthermore, under the imposed conditions, activation of slow-vacuolar (SV) cation channels in guard cell vacuoles requires~100 ,uM Ca2+ (Amodeo et al, 1994;Ward and Schroeder, 1994;Allen and Sanders, 1995). Therefore, SV channel currents did not interfere with the CDPK-activated currents under 0-50 jM Ca2+ in this study (Hedrich and Neher, 1987;Colombo et al, 1988;Ward and Schroeder, 1994;Allen and Sanders, 1995;Ward et al, 1995). At low cytosolic Ca2+ concentrations, whole-vacuole resistances were~I GQ (n >20), indicating small background currents that are typically found in guard cell vacuoles under most conditions (see also Ward and Schroeder, 1994;Allen and Sanders, 1995).…”

Section: Resultsmentioning

confidence: 70%

“…However, note that this apparent large anion permeability of SV channels results from the assumption that SV channels are Mg>2 impermeable, with 5 mM Mg2+ on both sides of the membrane (Schulz-Lessdorf and Hedrich, 1995). SV channels have a large relative permeability to Ca2+ (Ward and Schroeder, 1994;Allen and Sanders, 1995;Ward et al, 1995), and also to Mg2+ (Allen and Sanders, 1996;Z.-M.Pei and J.I.Schroeder, unpublished data). Therefore, permeability ratios in the above study for both anions and cations (Schulz-Lessdorf and Hedrich, 1995) are in need of re-examination.…”

Section: Do Sv Channels Transport Anions?mentioning

confidence: 99%

“…Also, comparison with (incorrectly quoted) average Ca2+: K+ permeability ratio quantities from other studies is not permissible, as the use of 5 mM Mg2+ greatly shifts reversal potentials invalidating these comparisons (Schulz-Lessdorf and Hedrich, 1995). Recent well-defined ion substitution experiments by several laboratories have shown SV channels to be largely anion impermeable in guard cell and other vacuoles (Kolb et al, 1987;Colombo et al, 1989;Lado et al, 1989;Amodeo et al, 1994;Allen and Sanders, 1995;Ward et al, 1995). Note that a small derived relative Clpermeability (e.g.…”

Section: Do Sv Channels Transport Anions?mentioning

confidence: 99%

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A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.

et al. 1996

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Calcium‐Dependent Protein Kinases (CDPKs) in higher plants contain a C‐terminal calmodulin‐like regulatory domain. Little is known regarding physiological CDPK targets. Both kinase activity and multiple Ca2+‐dependent signaling pathways have been implicated in the control of stomatal guard cell movements. To determine whether CDPK or other protein kinases could have a role in guard cell signaling, purified and recombinant kinases were applied to Vicia faba guard cell vacuoles during patch‐clamp experiments. CDPK activated novel vacuolar chloride (VCL) and malate conductances in guard cells. Activation was dependent on both Ca2+ and ATP. Furthermore, VCL activation occurred in the absence of Ca2+ using a Ca2+‐independent, constitutively active, CDPK* mutant. Protein kinase A showed weaker activation (22% as compared with CDPK). Current reversals in whole vacuole recordings shifted with the Nernst potential for Cl‐and vanished in glutamate. Single channel recordings showed a CDPK‐activated 34 +/− 5 pS Cl‐ channel. VCL channels were activated at physiological potentials enabling Cl‐ uptake into vacuoles. VCL channels may provide a previously unidentified, but necessary, pathway for anion uptake into vacuoles required for stomatal opening. CDPK‐activated VCL currents were also observed in red beet vacuoles suggesting that these channels may provide a more general mechanism for kinase‐dependent anion uptake.

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Section: Resultsmentioning

confidence: 70%

Section: Do Sv Channels Transport Anions?mentioning

confidence: 99%

Section: Do Sv Channels Transport Anions?mentioning

confidence: 99%

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A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.

et al. 1996

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“…In plants, TPC1 is highly expressed in all tissues, consistent with the ubiquity of SV channel activity in plant vacuoles. The voltage dependence of SV channels limits vacuolar Ca 2+ release, although recordings of deactivating (tail) currents show a clear Ca 2+ permeation toward the cytoplasmic membrane side (17, 180). For physiological Ca 2+ release (181), it has been proposed that the voltage dependence of SV channels would need to be shifted by modulators, as was recently found in Arabidopsis mesophyll vacuoles (182).…”

Section: Vacuolar Ion Channelsmentioning

confidence: 99%

Plant Ion Channels: Gene Families, Physiology, and Functional Genomics Analyses

Ward

Mäser

Schroeder

2009

Annu. Rev. Physiol.

325

262

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Distinct potassium, anion, and calcium channels in the plasma membrane and vacuolar membrane of plant cells have been identified and characterized by patch clamping. Primarily owing to advances in Arabidopsis genetics and genomics, and yeast functional complementation, many of the corresponding genes have been identified. Recent advances in our understanding of ion channel genes that mediate signal transduction and ion transport are discussed here. Some plant ion channels, for example, ALMT and SLAC anion channel subunits, are unique. The majority of plant ion channel families exhibit homology to animal genes; such families include both hyperpolarization-and depolarization-activated Shaker-type potassium channels, CLC chloride transporters/channels, cyclic nucleotide–gated channels, and ionotropic glutamate receptor homologs. These plant ion channels offer unique opportunities to analyze the structural mechanisms and functions of ion channels. Here we review gene families of selected plant ion channel classes and discuss unique structure-function aspects and their physiological roles in plant cell signaling and transport.

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“…However, plants can cope with abiotic stress via intricate mechanisms, including morphological, physiological, and molecular responses (Farooq et al, 2009). A large number of proteins have been reported to be involved in abiotic stress in plants, such as molecular chaperones (Wang et al, 2004), osmoregulatory proteins (Tamura et al, 2003), ion channel proteins (Ward et al, 1995), and transcription factors (Baldoni et al, 2015). Therefore, the identification and functional analysis of stress-associated proteins is helpful for plant breeders to cultivate stress-tolerant plants (Fujita et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

SiASR4, the Target Gene of SiARDP from Setaria italica, Improves Abiotic Stress Adaption in Plants

Yang

et al. 2017

Front. Plant Sci.

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Drought and other types of abiotic stresses negatively affect plant growth and crop yields. The abscisic acid-, stress-, and ripening-induced (ASR) proteins play important roles in the protection of plants against abiotic stress. However, the regulatory pathway of the gene encoding this protein remains to be elucidated. In this study, the foxtail millet (Setaria italica) ASR gene, SiASR4, was cloned and characterized. SiASR4 localized to the cell nucleus, cytoplasm and cytomembrane, and the protein contained 102 amino acids, including an ABA/WDS (abscisic acid/water-deficit stress) domain, with a molecular mass of 11.5 kDa. The abundance of SiASR4 transcripts increased after treatment with ABA, NaCl, and PEG in foxtail millet seedlings. It has been reported that the S. italica ABA-responsive DRE-binding protein (SiARDP) binds to a DNA sequence with a CCGAC core and that there are five dehydration-responsive element (DRE) motifs within the SiASR4 promoter. Our analyses demonstrated that the SiARDP protein could bind to the SiASR4 promoter in vitro and in vivo. The expression of SiASR4 increased in SiARDP-overexpressing plants. SiASR4-transgenic Arabidopsis and SiASR4-overexpressing foxtail millet exhibited enhanced tolerance to drought and salt stress. Furthermore, the transcription of stress-responsive and reactive oxygen species (ROS) scavenger-associated genes was activated in SiASR4 transgenic plants. Together, these findings show that SiASR4 functions in the adaption to drought and salt stress and is regulated by SiARDP via an ABA-dependent pathway.

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Roles of Ion Channels in Initiation of Signal Transduction in Higher Plants.

Cited by 261 publications

References 68 publications

A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.

A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.

Plant Ion Channels: Gene Families, Physiology, and Functional Genomics Analyses

SiASR4, the Target Gene of SiARDP from Setaria italica, Improves Abiotic Stress Adaption in Plants

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