Roles of hydroxyeicosatetraenoic acids in diabetes (HETEs and diabetes)

Dong, Linyue; Wang, He-Yao; Chen, Kaixian; Li, Yiming

doi:10.1016/j.biopha.2022.113981

Cited by 4 publications

(3 citation statements)

References 214 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While 11-HETE and 15- HETE are associated with COX metabolism [29], 15-HETE can also exhibit anti-inflammatory properties [27]. Additionally, while 12-HETE decreases insulin secretion in vitro [30], it is also decreased in obesity and inversely associated with arterial stiffness even after BMI adjustment [31]. Little is known about the metabolic impacts of oleic acid and alpha-linolenic acid-derived epoxides and diols, however, the analogous linoleate metabolites have received some attention.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic Responses to an Acute Glucose Challenge: The Differential Effects of Eight Weeks of Almond vs. Cracker Consumption in Young Adults

Dhillon,

Pandey,

Newman

et al. 2024

Preprint

View full text Add to dashboard Cite

This study investigated the dynamic responses to an acute glucose challenge following chronic almond versus cracker consumption for 8 weeks (clinicaltrials.gov ID:NCT03084003). Seventy-three young adults (age: 18-19 years, BMI: 18-41 kg/m2) participated in an 8-week randomized, controlled, parallel-arm intervention and were randomly assigned to consume either almonds (2 oz/d, n=38) or an isocaloric control snack of graham crackers (325 kcal/d, n=35) daily for 8 weeks. Twenty participants from each group underwent a 2-hour oral glucose tolerance test (oGTT) at the end of the 8-week intervention. Metabolite abundances in the oGTT serum samples were quantified using untargeted metabolomics, and targeted analyses for free PUFAs, total fatty acids, oxylipins, and endocannabinoids. Multivariate, univariate, and chemical enrichment analyses were conducted to identify significant metabolic shifts. Findings exhibit a biphasic lipid response distinguished by higher levels of unsaturated triglycerides in the earlier periods of the oGTT followed by lower levels in the latter period in the almond versus cracker group (p-value<0.05, chemical enrichment analyses). Almond (vs. cracker) consumption was also associated with higher AUC120 min of aminomalonate, and oxylipins (p-value<0.05), but lower AUC120 min of L-cystine, N-acetylmannosamine, and isoheptadecanoic acid (p-value<0.05). Additionally, the Matsuda Index in the almond group correlated with AUC120 min of CE 22:6 (r=-0.46; p-value<0.05) and 12,13 DiHOME (r=0.45; p-value<0.05). Almond consumption for 8 weeks leads to dynamic, differential shifts in response to an acute glucose challenge, marked by alterations in lipid and amino acid mediators involved in metabolic and physiological pathways.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Partial least squares discriminant analysis (PLS-DA) was conducted in JMP Pro (version 17.0) to identify shifts in metabolites over the oGTT time points (0, 15,30,60,120). Before the analysis, missing values were imputed using multivariate imputations [7].…”

Section: Multivariate and Univariate Analyses Of Metabolomics Datamentioning

confidence: 99%

Metabolic Responses to an Acute Glucose Challenge: The Differential Effects of Eight Weeks of Almond vs. Cracker Consumption in Young Adults

Dhillon,

Pandey,

Newman

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Cytochrome P450 (CYP) enzymes are a group of membrane-bound hemoproteins responsible for the synthesis of a great number of endogenous compounds including steroid hormones, bile acids, fatty acids, and eicosanoids [ 1 , 2 , 3 ]. CYPs are also major phase I metabolizing enzymes, bio-transforming xenobiotics such as drugs and carcinogens, in the body [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Heterologous Expression of Recombinant Human Cytochrome P450 (CYP) in Escherichia coli: N-Terminal Modification, Expression, Isolation, Purification, and Reconstitution

Tao

Fang

Ong

et al. 2023

BioTech

View full text Add to dashboard Cite

Cytochrome P450 (CYP) enzymes play important roles in metabolising endogenous and xenobiotic substances. Characterisations of human CYP proteins have been advanced with the rapid development of molecular technology that allows heterologous expression of human CYPs. Among several hosts, bacteria systems such as Escherichia coli (E. coli) have been widely used thanks to their ease of use, high level of protein yields, and affordable maintenance costs. However, the levels of expression in E. coli reported in the literature sometimes differ significantly. This paper aims to review several contributing factors, including N-terminal modifications, co-expression with a chaperon, selections of vectors and E. coli strains, bacteria culture and protein expression conditions, bacteria membrane preparations, CYP protein solubilizations, CYP protein purifications, and reconstitution of CYP catalytic systems. The common factors that would most likely lead to high expression of CYPs were identified and summarised. Nevertheless, each factor may still require careful evaluation for individual CYP isoforms to achieve a maximal expression level and catalytic activity. Recombinant E. coli systems have been evidenced as a useful tool in obtaining the ideal level of human CYP proteins, which ultimately allows for subsequent characterisations of structures and functions.

show abstract

Alteration of Hepatic Cytochrome P450 Expression and Arachidonic Acid Metabolism by Arsenic Trioxide (ATO) in C57BL/6 Mice

El-Ghiaty,

Alqahtani,

El-Mahrouk

et al. 2024

Biol Trace Elem Res

View full text Add to dashboard Cite

Roles of hydroxyeicosatetraenoic acids in diabetes (HETEs and diabetes)

Cited by 4 publications

References 214 publications

Metabolic Responses to an Acute Glucose Challenge: The Differential Effects of Eight Weeks of Almond vs. Cracker Consumption in Young Adults

Metabolic Responses to an Acute Glucose Challenge: The Differential Effects of Eight Weeks of Almond vs. Cracker Consumption in Young Adults

Heterologous Expression of Recombinant Human Cytochrome P450 (CYP) in Escherichia coli: N-Terminal Modification, Expression, Isolation, Purification, and Reconstitution

Alteration of Hepatic Cytochrome P450 Expression and Arachidonic Acid Metabolism by Arsenic Trioxide (ATO) in C57BL/6 Mice

Contact Info

Product

Resources

About