Roles of Helicobacter pylori BabA in gastroduodenal pathogenesis

Yamaoka, Yoshio

doi:10.3748/wjg.14.4265

Cited by 86 publications

(95 citation statements)

References 52 publications

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“…Nevertheless, it has been demonstrated in vitro that H. pylori strains with good adhesion properties could be babA2-negative by PCR probably due to allele variations (Olfat et al 2005). Yamaoka (2008b) has recommended immunoblot to evaluate BabA as well as the use of several primer pairs by PCR because of possible sequence variations.…”

Section: Discussionmentioning

confidence: 98%

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Association of iceA and babA genotypes in Helicobacter pylori strains with patient and strain characteristics

Boyanova

Yordanov

Gergova

et al. 2010

Antonie van Leeuwenhoek

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Data on the geographic prevalence of Helicobacter pylori iceA and babA alleles in Eastern Europe are still relatively scant. The aim of this study was to evaluate the prevalence of iceA and babA genotypes in Bulgarian symptomatic patients. The iceA and babA genotypes were evaluated by PCR with pure cultures in strains from 196 and 181 patients, respectively. Mixed infections were found in 10.2% of all 196 patients. Prevalence of H. pylori genotypes in patients with single-strain infections was 69.3% for iceA1, 30.7% for iceA2, 82.4% for cagA(+), 89.2% for vacA s1, 10.8% for vacA s2, 39.8% for vacA m1, 60.2% for vacA m2 and 48.8% for babA2. Within the iceA1 positive strains, 94.3% and 88.5% were also vacA s1a and cagA positive, respectively. Of the babA2 positive strains, 100.0%, 92.4% and 72.2% were also vacA s1a, cagA and iceA1 positive, respectively. Ulcer patients had more often strains with cagA positive status and vacA s1a allele. Although neither iceA1 nor babA2 were more common in ulcer patients, the combination of both alleles was more frequent (48.1%) in the ulcer patients than in the rest (28.7%). Clarithromycin susceptible strains had more often iceA1 allele (74.4%) than the resistant strains (55.3%). In conclusion, the results demonstrated a high prevalence of virulent H. pylori in Bulgaria. Both iceA1 and babA2 genotypes were associated with other virulence factors of H. pylori and, in addition, the iceA1 allele was associated with the strain susceptibility.

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Section: Discussionmentioning

confidence: 98%

“…BabA mediates the binding of H. pylori to fucosylated bloodgroup antigens Lewis b (Le b ) on the human gastric cells. Yamaoka (2008b) has observed BabA-high producers, which mediate Le b binding and BabA-low producers, which cannot bind to Le b .…”

Section: Introductionmentioning

confidence: 96%

Association of iceA and babA genotypes in Helicobacter pylori strains with patient and strain characteristics

Boyanova

Yordanov

Gergova

et al. 2010

Antonie van Leeuwenhoek

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“…In our study, babA2 was very rare and only associated with higher H. pylori density scores in the gastric body. To confirm the real prevalence of babA, some authors recommend immunoblot as well as the use of several primer pairs by PCR because of possible sequence variations [50,51].…”

Section: Discussionmentioning

confidence: 99%

Correlation of Helicobacter pylori Genotypes with Gastric Histopathology in the Central Region of a South-European Country

et al. 2014

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Background Outcome of Helicobacter pylori (H. pylori) infection results from interaction of multiple variables including host, environmental and bacterial-associated virulence factors. Aim This study aimed to investigate the correlation of cagA, cagE, vacA, iceA and babA2 genotypes with gastric histopathology and disease phenotype in the central region of a South-European country. Methods This prospective study involved 148 infected patients (110 female; mean age 43.5 ± 13.4 years) submitted to endoscopy with corpus and antrum biopsies. H. pylori was cultured and DNA extracted from the isolates. Genotypes were determined by PCR. Histopathological features were graded according to the updated Sydney system and OLGA/OLGIM classification. Only patients with single H. pylori genotypes and complete histopathological results were included.Results Antrum samples presented higher degrees of atrophy, intestinal metaplasia, chronic inflammation and neutrophil activity. Genotype distribution was as follows: cagA-31.8 %; cagE-45.9 %; vacA s1a-24.3 %; vacA s1b-19.6 %; vacA s1c-0.7 %; vacA s2-55.4 %; vacA m1-20.9 %; vacA m2-79.1 %; vacA s1m1-18.9 %; vacA s1m2-25.7 %; vacA s2m1-2 %; vacA s2m2-53.4 %; iceA1-33.8 %; iceA2-66.2 %; babA2-12.2 %. CagA genotype was significantly associated with higher degrees of intestinal metaplasia, neutrophil activity, chronic inflammation and OLGIM stages. BabA2 was linked with higher H. pylori density. Strains with vacA s1m1 or vacA s1m1 ? cagA positive genotypes had a significant association with peptic ulcer and vacA s2m2 with iron-deficient anemia. Conclusions cagA, vacA s1m1 and babA2 genotypes are relatively rare in the central region of Portugal. cagApositive strains are correlated with more severe histopathological modifications. This gene is commonly associated

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“…According to these results, the G27 strain, which possesses a tfs3a, might not produce DupA protein due to the presence of a stop codon in dupA (33). Similarly to DupA, expression of the blood group antigen binding adhesin (BabA) protein is not always correlated with babA gene expression (34). Further analysis of the dupA DNA sequence is necessary to clarify the significance of intact dupA.…”

Section: Discussionmentioning

confidence: 99%

The Intact dupA Cluster Is a More Reliable Helicobacter pylori Virulence Marker than dupA Alone

et al. 2012

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The duodenal ulcer promoting (dupA) gene, located in the plasticity region of Helicobacter pylori, is associated with duodenal ulcer development. dupA was predicted to form a type IV secretory system (T4SS) with vir genes around dupA (dupA cluster). We investigated the prevalence of dupA and dupA clusters and clarified associations between the dupA cluster status and clinical outcomes in the U.S. population. In all, 245 H. pylori strains were examined using PCR to evaluate the status of dupA and the adjacent vir genes predicted to form T4SS, in addition to the status of cag pathogenicity island (PAI). The associations between dupA cluster status and interleukin-8 (IL-8) and IL-12 production were also examined. The presence of dupA and all adjacent vir genes were defined as a complete dupA cluster. Many variations related to the status of dupA and dupA cluster genes were identified. Concurrent H. pylori infection and the presence of a complete dupA cluster increases duodenal ulcer risk compared to H. pylori infection with incomplete dupA cluster or without the dupA gene independent on the cag PAI status (adjusted odds ratio, 2.13; 95% confidence interval, 1.13 to 4.03). Gastric mucosal IL-8 levels were also significantly higher in the complete dupA cluster group than in other groups (P ‫؍‬ 0.01). In conclusion, although the causal relationship between the dupA cluster and duodenal ulcer development is not proved, the presence of a complete dupA cluster but not dupA alone, is associated with duodenal ulcer development.

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Roles of Helicobacter pylori BabA in gastroduodenal pathogenesis

Abstract: doi:10.3748/wjg.14.426

Cited by 86 publications

References 52 publications

Association of iceA and babA genotypes in Helicobacter pylori strains with patient and strain characteristics

Association of iceA and babA genotypes in Helicobacter pylori strains with patient and strain characteristics

Correlation of Helicobacter pylori Genotypes with Gastric Histopathology in the Central Region of a South-European Country

The Intact dupA Cluster Is a More Reliable Helicobacter pylori Virulence Marker than dupA Alone

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