Roles of FERONIA-like receptor genes in regulating grain size and quality in rice

Wang, Long; Wang, Dandan; Yang, Zhuhong; Jiang, Shengli; Qu, Jianing; He, Wei; Liu, Zhenming; Xing, Junjie; Ma, You-Chu; Lin, Qinlu; Yu, Feng

doi:10.1007/s11427-020-1780-x

Cited by 25 publications

(23 citation statements)

References 63 publications

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“…It has been unknown whether the CrRLK1L‐mediated ROS signaling pathway functions in pollen‐tube growth or the rupture of monocot species or not. Most of the CrRLK1L family members in rice have not been functionally characterized, except the FER and BUPS homologs (Li et al ., 2016; Pu et al ., 2017; Wang et al ., 2021; Yang et al ., 2020). Ruptured pollen tube (RUPO), an ortholog of Arabidopsis BUPS in rice, was reported to function in pollen‐tube growth and integrity, affecting K + homeostasis through the regulation of K + transporters such as OsHAK1/19/20 (Liu et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

OsMTD2‐mediated reactive oxygen species (ROS) balance is essential for intact pollen‐tube elongation in rice

Kim

Hong

et al. 2021

The Plant Journal

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SUMMARY The highly specialized haploid male gametophyte—pollen consist of two sperm cells and a large vegetative cell. Successful fertilization requires proper growth timing and rupture of the pollen tube until it delivers sperm cells, which occur immediately after a pollen grain hydrates. Although a tight regulation on polar cell‐wall expansion of the pollen tube is fundamentally important, the underlying molecular mechanism remains largely unknown, especially in crop plants. Here, we characterized the function of male‐gene transfer defective 2 (OsMTD2) gene in rice (Oryza sativa), which belongs to the plant‐specific receptor‐like kinase, the CrRLK1L family. We demonstrated that OsMTD2 is an essential male factor participating in pollen‐tube elongation based on genetic evidence and physiological observations. Because of unavailability of homozygous mutant via conventional methods, we used CRISPR‐Cas9 system to obtain homozygous knockout mutant of OsMTD2. We were able to identify phenotypic changes including male sterility due to early pollen‐tube rupture in the mutant. We observed that the production of reactive oxygen species (ROS) was dramatically reduced in mutants of OsMTD2 pollen grain and tubes with defective pectin distribution. Transcriptome analysis of osmtd2‐2 versus wild‐type anthers revealed that genes involved in defense responses, metabolic alteration, transcriptional and protein modification were highly upregulated in the osmtd2‐2 mutant. Through yeast‐two‐hybrid screening, we found that OsMTD2 kinase interacts with E3 ligase SPL11. Taken together, we propose that OsMTD2 has crucial functions in promoting pollen‐tube elongation through cell‐wall modification, possibly by modulating ROS homeostasis during pollen‐tube growth.

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Section: Introductionmentioning

confidence: 99%

OsMTD2‐mediated reactive oxygen species (ROS) balance is essential for intact pollen‐tube elongation in rice

Kim

Hong

et al. 2021

The Plant Journal

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“…Cr RLK1L homologues in rice have been shown to function in both redundant and complementary ways to regulate reproduction (Li et al ., 2016; Liu et al ., 2016a; Pu et al ., 2017) as well as grain size and quality (Wang et al ., 2021). FERONIA‐LIKE RECEPTOR (FLR) is the FER homologue in rice (Li et al ., 2016).…”

Section: Beyond Arabidopsis: Roles Of Crrlk1ls In Other Plant Lineagesmentioning

confidence: 99%

New paradigms in cell adaptation: decades of discoveries on the CrRLK1L receptor kinase signalling network

Zhu

et al. 2021

New Phytologist

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Receptor-like kinases (RLKs), which constitute the largest receptor family in plants, are essential for perceiving and relaying information about various environmental stimuli. Tremendous progress has been made in the past few decades towards elucidating the mechanisms of action of several RLKs, with emerging paradigms pointing to their roles in cell adaptations. Among these paradigms, Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) proteins and their rapid alkalinization factor (RALF) peptide ligands have attracted much interest. In particular, FERONIA (FER) is a CrRLK1L protein that participates in a wide array of physiological processes associated with RALF signalling, including cell growth and monitoring cell wall integrity, RNA and energy metabolism, and phytohormone and stress responses. Here, we analyse FER in the context of CrRLK1L members and their ligands in multiple species. The FER working model raises many questions about the role of CrRLK1L signalling networks during cell adaptation. For example, how do CrRLK1Ls recognize various RALF peptides from different organisms to initiate specific phosphorylation signal cascades? How do RALF-FER complexes achieve their specific, sometimes opposite, functions in different cell types? Here, we summarize recent major findings and highlight future perspectives in the field of CrRLK1L signalling networks.

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“…The product of the CDS fragment was then cloned into the pCAMBIA1300-GFP vector between the BamHI and XbaI sites. After verification by sequencing, the constructs were transferred into rice embryogenic calli via the Agrobacterium strain EHA105 to generate FLR1-OE lines (Wang et al, 2021). The resulting transgenic and wild-type (DJ) rice plants were grown in a greenhouse for 2 weeks and then transplanted into a field near the village of Taohua in Changsha, China (28…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…FERONIA (FER) is a member of the Catharanthus roseus RLK1-like (CrRLK1L) subfamily in Arabidopsis (Lindner et al, 2012) and participates in several biological processes, including reproduction and defense responses (Wolf and Hofte, 2014). FER regulates multiple biological responses through Ca 2+ signaling (Ngo et al, 2014;Shih et al, 2014;Feng et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…FERONIA-like receptor (FLR) members have been identified through genome-wide characterization of the CrRLK1L family in rice, and the sequences of the FLR1 and FLR2 proteins were found to be highly homologous to that of FER (Yang et al, 2020). FLR1 and FLR2 play distinct functions in fertility, grain size, and grain chalkiness (Li et al, 2016;Wang et al, 2021), and FLR1 and FLR2 are involved in the response to M. oryzae. Interestingly, FLR1 and FLR2 contribute to M. oryzae resistance phenotypes: the flr1 mutant exhibits a susceptible phenotype but the flr2 mutant exhibits a resistance phenotype (Huang et al, 2020;Yang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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FERONIA-like receptor 1-mediated calcium ion homeostasis is involved in the immune response

Luo

Wang

et al. 2022

Front. Plant Sci.

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Calcium (Ca2+) is the most abundant divalent cation in plants, and cellular levels of Ca2+, which functions as a nutrient and secondary messenger, play a critical role in plant immunity. In the present study, we found that FERONIA-like receptor 1 (FLR1) positively regulates Magnaporthe oryzae resistance and that expression of FLR1 is strongly induced in response to Ca2+ deficiency. In addition, the Ca content in the shoots of flr1 was lower than that in wild-type, and the M. oryzae-sensitive phenotype of the flr1 mutant was not rescued by exogenous application of Ca2+. Moreover, RNA sequencing revealed 2,697 differentially expressed genes (DEGs) in the flr1 mutant compared with wild-type, and some of these DEGs are involved in cellular metal ion homeostasis and transition metal ion homeostasis. Changes in expression of overlapping genes between the flr1 mutant and in plants under low-Ca2+ treatment were consistent in terms of direction, indicating that FLR1 is involved in Ca2+ homeostasis. In summary, we detected FLR1-mediated resistance to M. oryzae, a phenomenon associated with Ca2+ homeostasis.

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Roles of FERONIA-like receptor genes in regulating grain size and quality in rice

Cited by 25 publications

References 63 publications

OsMTD2‐mediated reactive oxygen species (ROS) balance is essential for intact pollen‐tube elongation in rice

OsMTD2‐mediated reactive oxygen species (ROS) balance is essential for intact pollen‐tube elongation in rice

New paradigms in cell adaptation: decades of discoveries on the CrRLK1L receptor kinase signalling network

FERONIA-like receptor 1-mediated calcium ion homeostasis is involved in the immune response

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