Roles of Exopolysaccharides and Lipopolysaccharides in the Adsorption of the Siphovirus Phage NM8 to Rhizobium meliloti M11S Cells

Defives, C.; Werquin, Michel; Mary, P.; Hornez, Jean‐Christophe

doi:10.1007/s002849900130

Cited by 19 publications

(15 citation statements)

References 22 publications

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“…Phage adsorption tests were carried out on the L. lactis strains SMQ-419, SMQ-420, SMQ-737, and SMQ-738, as described previously (11), but with the following modifications: GM17 broth was used, and the incubation temperature was 30°C. Phage adsorption assays were also performed on bacterial cells washed twice with 0.1% peptone (9). Detection of the eps gene cluster.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Exopolysaccharides on Phage-Host Interactions in Lactococcus lactis

Deveau

Calsteren

Moineau

2002

Appl Environ Microbiol

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In this study, we report that Lactococcus lactis strains producing exopolysaccharides (EPSstrains were insensitive to these phages. The monosaccharide composition of the polymer produced by the seven Eps ؉ strains was determined. The EPS produced by strains MLT3, SMQ-419, and SMQ-575 contained glucose, galactose, and rhamnose. The EPS fabricated by H414 contained only galactose. The EPS made by MLT2, SMQ-420, and SMQ-461 contained glucose and galactose. These findings indicate that the sugar composition of the EPS has no effect on phage sensitivity. The plasmid encoding the eps operon was cured from the two phage-sensitive strains. The cured derivatives were still phage sensitive, which indicates that EPS are not necessary for phage infection. Phage adsorption assays showed that the production of EPS does not confer a significant phage resistance phenotype.

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Section: Methodsmentioning

confidence: 99%

“…Specific phages infecting Escherichia coli and Vibrio cholerae strains that possess polysaccharide capsule have been isolated (2,8,30). In Rhizobium meliloti, the EPS production has been shown to inhibit phage adsorption (9).…”

mentioning

confidence: 99%

Effect of Exopolysaccharides on Phage-Host Interactions in Lactococcus lactis

Deveau

Calsteren

Moineau

2002

Appl Environ Microbiol

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“…EPS is presumed to provide a physical barrier between infecting phages and cell surface receptors (27,62). A protective function has been reported for Rhizobium meliloti, as EPS nonspecifically prevented phage adsorption (8). The physical removal of the K1 EPS capsule restored phage susceptibility of Escherichia coli (57).…”

Section: Discussionmentioning

confidence: 99%

“…Amylovorin was subsequently renamed amylovoran to be consistent with polysaccharide nomenclature (4). In due course, the linkages between E. amylovora's capsule, slime, polysaccharides, ooze, and pathogenicity were made (5,6,7,8,9,10). Growth of the bacterium on sucrose-, glucose-, or sorbitol-enriched media results in the production of excess quantities of two exopolysaccharides (EPSs), one acidic and one neutral (2,6,7).…”

Section: T He Common Characteristic Of Infection Of Rosaceous Plants Bymentioning

confidence: 99%

Host Exopolysaccharide Quantity and Composition Impact Erwinia amylovora Bacteriophage Pathogenesis

Roach

Sjaarda

Castle

et al. 2013

Appl Environ Microbiol

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Erwinia amylovora bacteriophages (phages) belonging to the Myoviridae and Podoviridae families demonstrated a preference for either high-exopolysaccharide-producing (HEP) or low-exopolysaccharide-producing (LEP) bacterial hosts when grown on artificial medium without or with sugar supplementation. Myoviridae phages produced clear plaques on LEP hosts and turbid plaques on HEP hosts. The reverse preference was demonstrated by most Podoviridae phages, where clear plaques were seen on HEP hosts. Efficiency of plating (EOP) was determined by comparing phage growth on the original isolation host to the that on the LEP or HEP host. Nine of 10 Myoviridae phages showed highest EOPs on LEP hosts, and 8 of 11 Podoviridae phages had highest EOPs on HEP hosts. Increasing the production of EPS on sugar-supplemented medium or decreasing production by knocking out the synthesis of amylovoran or levan, the two EPSs produced by E. amylovora, indicated that these components play crucial roles in phage infection. Amylovoran was virtually essential for proliferation of most Podoviridae phages when phage population growth was compared to the wild type. Decreased levan production resulted in a significant reduction of progeny from phages in the Myoviridae family. Thus, Podoviridae phages are adapted to hosts that produce high levels of exopolysaccharides and are dependent on host-produced amylovoran for pathogenesis. Myoviridae phages are adapted to hosts that produce lower levels of exopolysaccharides and host-produced levan.

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“…Along the same lines, GI8 also contains genes related with sialic acid synthesis genes, CMP-N-acetylneuraminic acid synthase (NeuA) and a sialic acid synthase (NeuB). Sialic acid has been shown in a-proteobacteria to influence phage adsorption (Defives et al, 1996). In Aeromonas, it has been described that Maf1, together with Maf-2, NeuB-like, FlmD and NeuAlike proteins are responsible for the glycosilation of polar and lateral flagella (Canals et al, 2007).…”

Section: Phage Resistance and Cell Surfacementioning

confidence: 99%

Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter

et al. 2008

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Alteromonas macleodii is a common marine heterotrophic c-proteobacterium. Isolates from this microbe cluster by molecular analysis into two major genotypic groups or ecotypes, one found in temperate latitudes in the upper water column and another that is for the most part found in the deep water column of the Mediterranean. Here, we describe the genome of one strain of the 'deep ecotype' (AltDE) isolated from 1000 m in the Eastern Mediterranean and compare this genome with that of the type strain ATCC 27126, a representative of the global 'surface' ecotype. The genomes are substantially different with DNA sequence similarity values that are borderline for microbes belonging to the same species, and a large differential gene content, mainly found in islands larger than 20 kbp, that also recruit poorly to the Global Ocean Sampling project (GOS). These genomic differences indicate that AltDE is probably better suited to microaerophilic conditions and for the degradation of recalcitrant compounds such as urea. These, together with other features, and the distribution of this genotypic group, indicate that this microbe colonizes relatively large particles that sink rapidly to meso and bathypelagic depths. The genome of ATCC 27126 on the other hand has more potential for regulation (two component systems) and degrades more sugars and amino acids, which is consistent with a more transient particle attachment, as would be expected for lineages specialized in colonizing smaller particulate organic matter with much slower sinking rates. The genomic data are also consistent with a picture of incipient speciation driven by niche specialization.

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Roles of Exopolysaccharides and Lipopolysaccharides in the Adsorption of the Siphovirus Phage NM8 to Rhizobium meliloti M11S Cells

Cited by 19 publications

References 22 publications

Effect of Exopolysaccharides on Phage-Host Interactions in Lactococcus lactis

Effect of Exopolysaccharides on Phage-Host Interactions in Lactococcus lactis

Host Exopolysaccharide Quantity and Composition Impact Erwinia amylovora Bacteriophage Pathogenesis

Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter

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