Roles of ESCRT-III polymers in cell division across the tree of life

Carlton, Jeremy Graham; Baum, Buzz

doi:10.1016/j.ceb.2023.102274

Cited by 5 publications

(6 citation statements)

References 129 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…32,33 The ESCRT-IIIA group (hereafter A-type) contains CHMP1/Vps46/Did2, CHMP2/Vps2, CHMP3/Vps24, and IST1, while the ESCRT-IIIB group (hereafter B-type) contains CHMP4/Vps32/Snf7, CHMP5/Vps60, CHMP6/Vps20, and CHMP7/CHM7. 1,32,50 The topology of the tree, together with previous results, 1 suggests a simple model of ESCRT-III evolution in which an ancestral gene was duplicated in the common ancestor of all Asgard archaea, giving rise to two specialised ESCRT-III proteins (Figure 1B). Over the course of several rounds of gene duplication and diversification during eukaryogenesis, these paralogues gave rise to the complete eukaryotic set (Figure 1B).…”

Section: Resultssupporting

confidence: 61%

“…Since the discovery of ESCRT-III proteins in eukaryotes, 7,12 numerous studies have examined ESCRT-III structure, biochemistry, and ESCRT-III-dependent membrane remodelling. While there were a few unique findings in these data, including polymers that contain subunits in the closed conformation, 42,44,45 which have different membrane binding interfaces, 42,44,66 or engage with membranes of opposite curvature to those in the cell, 49,50 a common set of features has emerged.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evolutionarily conserved principles of ESCRT-III-mediated membrane remodelling revealed by a two-subunit Asgard archaeal system

Souza,

Espadas,

Chaaban

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

SummaryESCRT-III proteins assemble into composite polymers that undergo stepwise changes in composition and structure to deform membranes across the tree of life. Here, using a phylogenetic analysis we demonstrate that the two ESCRT-III proteins present in our closest archaeal relatives are evolutionarily related to B-type and A-type eukaryotic paralogues, which initiate and execute membrane remodelling, respectively. This deep homology is reflected in ESCRT-III structure and function as demonstrated by the fact that ESCRT-IIIB assembles into parallel arrays on planar membranes to initiate membrane deformation, and is required to recruit ESCRT-IIIA to generate composite polymers. ESCRT-IIIA homopolymers can then remodel membranes into tubes, as a likely prelude to scission. Taken together, this analysis reveals a set of conserved principles governing ESCRT-III-dependent membrane remodelling that first emerged with the evolution of a two-component ESCRT-III system in the Asgard archaea, and which continue to underlie complex multi-component, ESCRT-III-dependent membrane remodelling in eukaryotes.

show abstract

Section: Resultssupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Evolutionarily conserved principles of ESCRT-III-mediated membrane remodelling revealed by a two-subunit Asgard archaeal system

Souza,

Espadas,

Chaaban

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Otherwise, MVBs are routed to the lysosomes where their content is degraded, including membrane receptors [ 33 , 34 ]. The ILV genesis process relies on an intricate protein machinery called the Endosomal Sorting Complex Required for Transport (ESCRT) which reshapes the endosomal membrane topology [ 35 , 36 ]. During MVB formation, the ESCRT allows the intraluminal budding of vesicles and the sorting of embedded cargo proteins.…”

Section: Exosomes Are Small Specialized Extracellular Vesicles (Evs)mentioning

confidence: 99%

Exosome-Mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control and Vaccine Design

El Safadi,

Mokhtari,

Krejbich

et al. 2024

Vaccines

View full text Add to dashboard Cite

Exosomes are small subtypes of extracellular vesicles (EVs) naturally released by different types of cells into their environment. Their physiological roles appear to be multiple, yet many aspects of their biological activities remain to be understood. These vesicles can transport and deliver a variety of cargoes and may serve as unconventional secretory vesicles. Thus, they play a crucial role as important vectors for intercellular communication and the maintenance of homeostasis. Exosome production and content can vary under several stresses or modifications in the cell microenvironment, influencing cellular responses and stimulating immunity. During infectious processes, exosomes are described as double-edged swords, displaying both beneficial and detrimental effects. Owing to their tractability, the analysis of EVs from multiple biofluids has become a booming tool for monitoring various pathologies, from infectious to cancerous origins. In this review, we present an overview of exosome features and discuss their particular and ambiguous functions in infectious contexts. We then focus on their properties as diagnostic or therapeutic tools. In this regard, we explore the capacity of exosomes to vectorize immunogenic viral antigens and their function in mounting adaptive immune responses. As exosomes provide interesting platforms for antigen presentation, we further review the available data on exosome engineering, which enables peptides of interest to be exposed at their surface. In the light of all these data, exosomes are emerging as promising avenues for vaccine strategies.

show abstract

“…Routing to the lysosome enables ILV components to be degraded, which in turn allows turnover of membrane receptors [27,28]. The whole process requires an intricate protein machinery called the Endosomal Sorting Complex Required for Transport (ESCRT) which reshapes the endosomal membrane topology [29,30]. During MVB formation, ESCRT allows intraluminal budding of vesicles with the sorting of embedded cargo proteins.…”

Section: Exosomes Biogenesismentioning

confidence: 99%

Exosome-mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control

EL SAFADI,

KREJBICH,

MOKHTARI

et al. 2024

Preprint

View full text Add to dashboard Cite

Exosomes are small subtypes of extracellular vesicles (EVs) naturally released by different types of cells into their environment. Their physiological roles appear to be multiple, and many aspects of their biological activities remain to be understood. These vesicles can transport and deliver a variety of cargoes, including proteins, metabolites, nucleic acids, and lipids. Consequently, it is argued that exosomes may serve as unconventional secretory vesicles, playing a crucial role as important vectors for intercellular communication and the maintenance of homeostasis. Exosome production and content can vary under several stresses or modifications in the cell microenvironment, influencing cellular responses both qualitatively and quantitatively. Thus, the analysis of EVs from multiple biofluids, due to its ease of implementation, has become a booming tool for monitoring various pathologies, particularly cancer, as we shall outline further on. During infectious processes, exosomes are described as double-edged swords, displaying both beneficial and detrimental effects. Indeed, during a viral infection, the production of EVs and circulating exosomes is often enhanced to signal danger, stimulate immunity, and possibly serve as pathological biomarkers. Conversely, viruses can disrupt or hijack the exosome content and production. In this review, we discuss the particular and ambiguous functions of exosomes in infectious contexts. Furthermore, we explore the vectoring capacity of exosomes in light of the extensive literature examining their function in mounting adaptive immune responses. Exosomes provide an interesting platform for antigen presentation and could therefore serve as novel therapeutic targets or be used in vaccine strategies.

show abstract

Roles of ESCRT-III polymers in cell division across the tree of life

Cited by 5 publications

References 129 publications

Evolutionarily conserved principles of ESCRT-III-mediated membrane remodelling revealed by a two-subunit Asgard archaeal system

Evolutionarily conserved principles of ESCRT-III-mediated membrane remodelling revealed by a two-subunit Asgard archaeal system

Exosome-Mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control and Vaccine Design

Exosome-mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control

Contact Info

Product

Resources

About