Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis

Adams, Ralf H.; Wilkinson, George A.; Weiss, Cornelia; Diella, Francesca; Gale, Nicholas W.; Deutsch, Urban; Risau, Werner; Klein, Rüdiger

doi:10.1101/gad.13.3.295

Cited by 940 publications

(833 citation statements)

References 53 publications

Supporting

Mentioning

806

Contrasting

Unclassified

Order By: Relevance

“…EphrinB2, a transmembrane protein endowed with its own signaling potential, is expressed on arterial and lymphatic endothelial cells (ECs) as well as perivascular cells, and its receptor, EphB4, is largely confined to venous and lymphatic endothelium (Wang et al, 1998;Adams et al, 1999;Gerety et al, 1999;Makinen et al, 2005) Experiments using gene targeted mice have shown that these proteins are crucial for embryonic sprouting angiogenesis, and together with other family members could contribute greatly to specialization and maturation of vascular beds (Heroult et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…The final vascular response to ephrinB2 engagement with its receptors on adjacent cells is shaped by bidirectional signaling interactions within endothelial populations and between endothelial cells and adjacent nonendothelial populations (Heroult et al, 2006). The interaction between ephrinB2 and EphB4 within vascular endothelia may provide the critical repulsion signals required to set arterial-venous boundaries, reminiscent of their repulsive guidance of axonal growth cones (Wang et al, 1998;Adams et al, 1999;Gerety et al, 1999;Gerety and Anderson, 2002). However, ephrinB-Eph interaction can also stimulate endothelial cell sprouting (Adams et al, 1999;Palmer et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…The interaction between ephrinB2 and EphB4 within vascular endothelia may provide the critical repulsion signals required to set arterial-venous boundaries, reminiscent of their repulsive guidance of axonal growth cones (Wang et al, 1998;Adams et al, 1999;Gerety et al, 1999;Gerety and Anderson, 2002). However, ephrinB-Eph interaction can also stimulate endothelial cell sprouting (Adams et al, 1999;Palmer et al, 2002). Endothelial ephrinB2 can also interact with receptors on mural cells or perivascular mesenchyme to affect vascular development (Adams et al, 1999;Oike et al, 2002;Umeda et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…However, ephrinB-Eph interaction can also stimulate endothelial cell sprouting (Adams et al, 1999;Palmer et al, 2002). Endothelial ephrinB2 can also interact with receptors on mural cells or perivascular mesenchyme to affect vascular development (Adams et al, 1999;Oike et al, 2002;Umeda et al, 2004). Finally, ephrinB2 on nonvascular cells can also affect vascular patterning (Zhang et al, 2001;Foo et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Role for ephrinB2 in postnatal lung alveolar development and elastic matrix integrity

Wilkinson

Schittny

Reinhardt

et al. 2008

Developmental Dynamics

View full text Add to dashboard Cite

Alveoli are formed in the lung by the insertion of secondary tissue folds, termed septa, which are subsequently remodeled to form the mature alveolar wall. Secondary septation requires interplay between three cell types: endothelial cells forming capillaries, contractile interstitial myofibroblasts, and epithelial cells. Here, we report that postnatal lung alveolization critically requires ephrinB2, a ligand for Eph receptor tyrosine kinases expressed by the microvasculature. Mice homozygous for the hypomorphic knockin allele ephrinB2 ⌬V/⌬V , encoding mutant ephrinB2 with a disrupted C-terminal PDZ interaction motif, show severe postnatal lung defects including an almost complete absence of lung alveoli and abnormal and disorganized elastic matrix. Lung alveolar formation is not sensitive to loss of ephrinB2 cytoplasmic tyrosine phosphorylation sites. Postnatal day 1 mutant lungs show extracellular matrix alterations without differences in proportions of major distal cell populations. We conclude that lung alveolar formation relies on endothelial ephrinB2 function. Developmental Dynamics 237:2220 -2234, 2008.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Role for ephrinB2 in postnatal lung alveolar development and elastic matrix integrity

Wilkinson

Schittny

Reinhardt

et al. 2008

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…Toutes sont également exprimées dans le système nerveux où elles contrô-lent la destinée et le guidage des précur-seurs neuronaux et des axones, ce qui suggère leur rôle dans l'établissement du réseau vasculaire. Les CE artérielles du poisson zèbre, du poulet et de la souris expriment sélectivement l'éphrine-B2, la neuropiline 1 (NRP1), le récepteur notch3, le ligand de Notch Delta-like 4 et gridlock [5][6][7][8][9][10][11][9][10][11][12][13][14][15][16]. D'autres molécules sont spécifiquement exprimées par les CE veineuses, notamment EphB4, le récepteur du marqueur artériel éphrine-B2 [12].…”

Section: Les Marqueurs Moléculaires Spécifiques Des Artères Et Des Veunclassified

Différenciation artérioveineuse : génétique ou hémodynamique ?

2004

View full text Add to dashboard Cite

> Le système vasculaire adulte comprend trois compartiments: artériel, veineux et lymphatique, mais jusqu'à récemment, le contrôle de l'identité de ces vaisseaux était mal connu. Au cours du développe-ment embryonnaire, la mise en place du réseau artérioveineux s'effectue très précocement, peu après les premiers battements cardiaques, afin d'assurer la circulation embryonnaire. La décou-verte récente de marqueurs artériels et veineux spécifiques pouvait laisser supposer une prédétermination des cellules endothéliales (CE) à participer soit à une artère, soit à une veine et, de ce fait, un destin irréversiblement fixé par des facteurs génétiques. En dépit de l'expression de ces gènes spécifiques, nous avons montré que les CE gardent une plasticité vis-à-vis de leur différen-ciation artérielle ou veineuse, et ce en relation avec des facteurs hémodyna-miques.

show abstract

Characterization of the Larynx in Ephrin-B2 Knockout Mice

Neilan

Shao

Dravis

et al. 2012

Arch Otolaryngol Head Neck Surg

View full text Add to dashboard Cite

Subjects: 129/CD1 mice with the ephrin-B2 gene disrupted by the ␤-galactosidase (lacZ) gene were humanely killed at embryonic day 18 (E18) and evaluated for the presence and characterization of a laryngeal cleft. Homozygous and heterozygous lacZ knockout mice as well as wild-type littermates were evaluated. Main Outcome Measures: Microsurgical dissection of the oral cavity and pharynx allowed for a pseudoen-doscopic view of the larynx to determine the presence or absence of a cleft. The specimens were also histologically sectioned and examined for characterization and classification of the cleft. Results: A laryngeal cleft was identified in 12 of 27 ephrin-B2 homozygous lacZ knockout mice (44%). Laryngeal clefts were not identified in heterozygous ephrin-B2 knockout mice or in wild-type littermates. Conclusions: Disruption of ephrin-B2 reverse signaling results in laryngeal clefts in lacZ knockout mice. This presents a novel mouse model in which future investigations into etiology of laryngeal clefts may be examined.

show abstract

Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis

Cited by 940 publications

References 53 publications

Role for ephrinB2 in postnatal lung alveolar development and elastic matrix integrity

Role for ephrinB2 in postnatal lung alveolar development and elastic matrix integrity

Différenciation artérioveineuse : génétique ou hémodynamique ?

Characterization of the Larynx in Ephrin-B2 Knockout Mice

Contact Info

Product

Resources

About