Roles of dental pulp fibroblasts in the recognition of bacterium-related factors and subsequent development of pulpitis

Nakanishi, Tadashi; Takegawa, Daisuke; Hirao, Kazuyuki; Takahashi, Kanako; Yumoto, Hiromichi; Matsuo, Takashi

doi:10.1016/j.jdsr.2011.02.001

Cited by 24 publications

(24 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…, Nakanishi et al . ). Although there is extensive homology in the soluble factors released by nemotic and inflammatory pulp cells, further studies are needed to compare nemosis process and dental pulp inflammation to know if they are both connected.…”

Section: Discussionmentioning

confidence: 97%

“…Fibroblasts are heterogeneous cells able to activate and to be involved in inflammation and immune reactions (Silzle et al 2004, Staquet et al 2008. These cells are known to have great plasticity and to be the predominant cell type in the dental pulp (Goldberg & Smith 2004, Goldberg et al 2008, Nakanishi et al 2011, Osonoi et al 2011. In this study, the purpose was to highlight the nemosis process in dental pulp and lung fibroblasts spheroids to compare this type of cell death in these two cell populations.…”

Section: Discussionmentioning

confidence: 99%

“…This fact may be explained by the need of dental pulp fibroblasts to have interactions with other cells such as odontoblasts, crucial for the release of some soluble factors. Nemotic fibroblasts also produce extracellular proteolytic enzymes such as matrix metalloproteinases and many cytokines and chemokines able to recruit specific populations of leucocytes during inflammation, including initiation and progression of pulpitis (Smith et al 1997, Nakanishi et al 2011. Although there is extensive homology in the soluble factors released by nemotic and inflammatory pulp cells, further studies are needed to compare nemosis process and dental pulp inflammation to know if they are both connected.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of a programmed necrosis process in 3‐dimensional cultures of dental pulp fibroblasts

Clerc¹,

Pérard²,

Pellen‐Mussi

et al. 2012

Int Endodontic J

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of a programmed necrosis process in 3‐dimensional cultures of dental pulp fibroblasts

Clerc¹,

Pérard²,

Pellen‐Mussi

et al. 2012

Int Endodontic J

View full text Add to dashboard Cite

show abstract

“…As the major cells in the dental pulp tissue (Nakanishi et al . ), these mesenchymal cells are critical for the homeostasis of this connective tissue and are responsible for the repair process in case of chemical, mechanical and microbial injuries. Long considered to be solely support cells, fibroblasts are now considered as active cells involved in wound healing, angiogenesis, inflammatory and immune processes in many tissues (Smith et al .…”

Section: Introductionmentioning

confidence: 99%

Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts

et al. 2017

View full text Add to dashboard Cite

Le Clerc J, Tricot-Doleux S, Pellen-Mussi P, P erard M, Jeanne S, P erez F. Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts. International Endodontic Journal, 51, e94-e106, 2018.Aim To investigate in human dental pulp fibroblasts (HDPF) the expression of factors involved in dental pulp physiopathological processes and in an experimental model of cell activation called nemosis, and to compare the behaviour of pulp cell activation with sound lung fibroblast MRC5, employed as a reference model for nemosis. Methodology Nemotic response was induced in three-dimensional cultures of HDPF and lung fibroblasts. The expressions of molecules involved in physiological (alkaline phosphatase, type I collagen) and in inflammatory processes (IL-6, CXCL8, CCL20, COX-2) were studied using real-time PCR. Concentrations of IL-6 and CXCL8 were analysed during 4 days with ELISA. Nonparametric tests were used to determine statistical differences between groups. Results A significant decrease (P < 0.001) in type I collagen and alkaline phosphatase was observed in MRC5 and HDPF nemotic responses. Although the amounts of mRNA differed between these cell types, there was an increase in CCL20, CXCL8 and COX-2 expression (P < 0.001). Unlike HDPF, MRC5 spheroids displayed significant amounts of IL-6 concentrations and mRNA expression. Notably, increased concentrations of CXCL8 were recorded in all threedimensional cultures compared with monolayers as a function of time (P < 0.05). Conclusion Although the nemotic responses observed were not identical in the pulpal and lung fibroblasts, similarities occurred in the expression of chemokines and cyclooxygenase-2. Nemotic reactions and inflammatory processes in pulp diseases share similarities in terms of the expression of factors. Thus, this in vitro model could constitute a powerful tool to study intercellular relations within the dental pulp and to develop new local treatments to counteract the inflammatory reaction that occurs during pulpitis.

show abstract

“…Asimismo ha sido reportada la síntesis de mediadores de inflamación y citoquinas en respuesta a componentes bacterianos (Nakanishi et al, 2011), contribuyendo además a la inmunidad innata (Takeuchi & Akira, 2010). Si bien su función principal es secretora, ante injuria pulpar, estas células son las primeras en ir en reemplazo de los odontoblastos destruidos y diferenciarse a estos para suplir su función (Vavpotic et al, 2009;Nakanishi et al), por lo cual los FP pueden ser considerados células odontoprogenitoras (Liwei et al, 2016).…”

Section: Introductionunclassified

Pulpa Dentaria Sana vs. Pulpitis Reversible: Caracterización Estereológica de Fibroblastos

Trujillo¹,

Morales²,

Roa³

2016

Int. J. Morphol.

View full text Add to dashboard Cite

MORALES, R. & ROA, I. Pulpa dentaria sana vs. pulpitis reversible: caracterización estereológica de fibroblastos. Int. J. Morphol., 34(3):945-949, 2016. RESUMEN:La reacción y reparación de la dentina depende del número de células presentes en la pulpa, dentro de éstas fibroblastos. Los métodos diseñados para obtener una estimación fiable de la cantidad de elementos celulares de la pulpa han sido subjetivos y sesgados, sobre todo al evaluar los cambios cuantitativos y potencial capacidad reparadora en presencia de caries. El objetivo fue estimar y comparar cuantitativamente las densidades de número, volumen y superficie de fibroblastos en pulpas sanas y con diagnóstico de pulpitis reversible producto de caries en dientes humanos jóvenes. Se utilizaron dientes premolares humanos obtenidos de exodoncias, divididos en un grupo sano y cariado, los cuales fueron fijados y posteriormente descalcificados con ácido nítrico al 5 %. Siguiendo el protocolo del orientator se obtuvieron 5 secciones de 5 µm teñidas por H-E de cada diente. Se aplicó el recuento estereológico de los fibroblastos pulpares (FP) con el test multipropósito M42. Se estimaron las densidades de número (Nv), volumen (Vv) y superficie (Sv), y calcularon las Medias (±DE) por diente, y Medias (±EE) por grupo. Las diferencias entre grupos se analizaron mediante la prueba T, con un valor p ≤0,05 de significación estadística. En dientes sanos, la Media (±EE) para Nv de FP fue 0,393 x 10 5 /mm 3 (±0,020x10 ). Al comparar las Nv, los FP del grupo con caries aumentaron significativamente (p= 0,047), al igual que la Vv (p= 0,0105) y Sv (p= 0,013). Existe un aumento del número de FP en los dientes con pulpitis reversible, lo que condicionaría su capacidad de respuesta. La metodología empleada puede ser aplicable para determinar el comportamiento pulpar y cuantificar variables de respuesta odontoblástica en tratamientos restauradores atraumáticos de manera imparcial y reproducible.

show abstract

Roles of dental pulp fibroblasts in the recognition of bacterium-related factors and subsequent development of pulpitis

Cited by 24 publications

References 71 publications

Characterization of a programmed necrosis process in 3‐dimensional cultures of dental pulp fibroblasts

Characterization of a programmed necrosis process in 3‐dimensional cultures of dental pulp fibroblasts

Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts

Pulpa Dentaria Sana vs. Pulpitis Reversible: Caracterización Estereológica de Fibroblastos

Contact Info

Product

Resources

About