Roles of cytochromes P450 in plant reproductive development

Distefano, Ayelén Mariana; Setzes, Nicolás; Cascallares, María Milagros; Fiol, Diego Fernando; Zabaleta, Eduardo; Pagnussat, Gabriela Carolina

doi:10.1387/ijdb.200100gp

Cited by 10 publications

(11 citation statements)

References 44 publications

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“…As such, eight genes were identified as candidates and all were associated with branching, branch type, and branching level traits. For example, Manes.18G016700 (aldehyde oxidase GLOX) as well as Manes.18G016725 (lysine-specific histone demethylase 1 homolog 3) and Manes.18G016200 (cytochrome P450 83B1), all of which are associated with SNP S18_1832353 on chromosome 18, are involved in anther and pollen development [ 60 ], promotion of the floral transition [ 61 ], auxin homeostasis, and reproductive development [ 62 , 63 ], respectively in A. thaliana . Manes.05G186700 (protein DETOXIFICATION 48) associated with SNP S5_29309724 on chromosome 5 is involved in specifying the lateral organ initiation rate in A. thaliana [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

“…Auxins are plant growth regulators integrated within the gene regulatory networks that control most aspects of plant development, including coordinated organ outgrowth and initiation of floral meristems [ 95 ]. Additionally, cytochrome P450 proteins, which are widely distributed in plants, participate in a vast array of pathways leading to the synthesis and modification of multiple metabolites with variable and important functions during different stages of plant development [ 63 ]. Meanwhile, although the role of Manes.18G016700 (aldehyde oxidase GLOX, Germin-like oxalate oxidases) in plants is not yet fully understood, it is believed to be involved in anther development, during which it plays a role in tapetum and pollen development in A. thaliana [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

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Identification of Genomic Regions for Traits Associated with Flowering in Cassava (Manihot esculenta Crantz)

Baguma,

Mukasa,

Nuwamanya

et al. 2024

Plants

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Flowering in cassava (Manihot esculenta Crantz) is crucial for the generation of botanical seed for breeding. However, genotypes preferred by most farmers are erect and poor at flowering or never flower. To elucidate the genetic basis of flowering, 293 diverse cassava accessions were evaluated for flowering-associated traits at two locations and seasons in Uganda. Genotyping using the Diversity Array Technology Pty Ltd. (DArTseq) platform identified 24,040 single-nucleotide polymorphisms (SNPs) distributed on the 18 cassava chromosomes. Population structure analysis using principal components (PCs) and kinships showed three clusters; the first five PCs accounted for 49.2% of the observed genetic variation. Linkage disequilibrium (LD) estimation averaged 0.32 at a distance of ~2850 kb (kilo base pairs). Polymorphism information content (PIC) and minor allele frequency (MAF) were 0.25 and 0.23, respectively. A genome-wide association study (GWAS) analysis uncovered 53 significant marker–trait associations (MTAs) with flowering-associated traits involving 27 loci. Two loci, SNPs S5_29309724 and S15_11747301, were associated with all the traits. Using five of the 27 SNPs with a Phenotype_Variance_Explained (PVE) ≥ 5%, 44 candidate genes were identified in the peak SNP sites located within 50 kb upstream or downstream, with most associated with branching traits. Eight of the genes, orthologous to Arabidopsis and other plant species, had known functional annotations related to flowering, e.g., eukaryotic translation initiation factor and myb family transcription factor. This study identified genomic regions associated with flowering-associated traits in cassava, and the identified SNPs can be useful in marker-assisted selection to overcome hybridization challenges, like unsynchronized flowering, and candidate gene validation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of Genomic Regions for Traits Associated with Flowering in Cassava (Manihot esculenta Crantz)

Baguma,

Mukasa,

Nuwamanya

et al. 2024

Plants

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“…Through coevolution with their host plants, herbivorous insects may develop detoxification defense strategies, particularly the cytochrome P450 monooxygenases (P450s), GSTs, and carboxy/cholinesterases (Strode et al ., 2008; Huang et al ., 2011). For example, P450 is associated with the biosynthesis of many metabolites implicated in a variety of physiological responses such as signaling and defense (Distéfano et al ., 2021). Overexpression of the detoxifying enzyme genes P450 and GST are important in N. lugens resistance using increased metabolic ability (Vontas et al ., 2002; Zhang et al ., 2016).…”

Section: Discussionmentioning

confidence: 99%

Cardinium infection alters cotton defense and detoxification metabolism of its whitefly host

et al. 2022

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Field monitoring revealed that the infection ratio of the bacterial symbiont Cardinium in the whitefly (Bemisia tabaci MED) was relatively low in northern China. However, the role of this symbiont and the symbiont-whitefly-host plant interaction mechanism are poorly understood. We investigated the influence of Cardinium on the competitiveness of the host whitefly and the physiological interaction between the host plants and host whiteflies. Cardinium-infected whiteflies were displaced by uninfected whiteflies after 5 generations, which showed that Cardinium infection reduced whitefly competitiveness. The defense response genes of cotton significantly decreased under infestation by infected whiteflies compared to uninfected whiteflies. The expression of detoxification metabolism genes, especially the uridine 5ʹ-diphosphoglucuronyltransferase and P450 genes, in infected whiteflies significantly decreased. These results demonstrated that Cardinium could inhibit the defense response of the host plant and decrease the detoxification metabolism ability of the host whitefly. The reduced competitiveness of infected whiteflies may be associated with the inhibition of the whitefly detoxification metabolism by Cardinium, resulting in the reduced performance of infected whiteflies. However, Cardinium infection can suppress plant defenses, which may benefit both infected and uninfected whiteflies when they coexist. This research illustrates the symbiont-whitefly-host plant interaction mechanism and the population dynamics of the whitefly.

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“…Since most bacterial and fungal P450s are cytosolic and soluble, these variants are better suited for biotechnology applications compared to their membrane-bound plant and mammalian homologs (Distefano et al 2021;Finnigan et al 2020;Iizaka et al 2021;Toplak et al 2021;Zhang et al 2021b). In P. megaterium a number of different P450 enzymes were found.…”

Section: Secretion Of Recombinant Proteins With P Megateriummentioning

confidence: 99%

The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology

Biedendieck

Knuuti

Jahn

2021

Appl Microbiol Biotechnol

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Over 30 years, the Gram-positive bacterium Priestia megaterium (previously known as Bacillus megaterium) was systematically developed for biotechnological applications ranging from the production of small molecules like vitamin B12, over polymers like polyhydroxybutyrate (PHB) up to the in vivo and in vitro synthesis of multiple proteins and finally whole-cell applications. Here we describe the use of the natural vitamin B12 (cobalamin) producer P. megaterium for the elucidation of the biosynthetic pathway and the subsequent systematic knowledge-based development for production purposes. The formation of PHB, a natural product of P. megaterium and potential petro-plastic substitute, is covered and discussed. Further important biotechnological characteristics of P. megaterium for recombinant protein production including high protein secretion capacity and simple cultivation on value-added carbon sources are outlined. This includes the advanced system with almost 30 commercially available expression vectors for the intracellular and extracellular production of recombinant proteins at the g/L scale. We also revealed a novel P. megaterium transcription-translation system as a complementary and versatile biotechnological tool kit. As an impressive biotechnology application, the formation of various cytochrome P450 is also critically highlighted. Finally, whole cellular applications in plant protection are completing the overall picture of P. megaterium as a versatile giant cell factory. Key points • The use of Priestia megaterium for the biosynthesis of small molecules and recombinant proteins through to whole-cell applications is reviewed. • P. megaterium can act as a promising alternative host in biotechnological production processes.

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Roles of cytochromes P450 in plant reproductive development

Cited by 10 publications

References 44 publications

Identification of Genomic Regions for Traits Associated with Flowering in Cassava (Manihot esculenta Crantz)

Identification of Genomic Regions for Traits Associated with Flowering in Cassava (Manihot esculenta Crantz)

Cardinium infection alters cotton defense and detoxification metabolism of its whitefly host

The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology

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