Roles of curli, cellulose and BapA in Salmonella biofilm morphology studied by atomic force microscopy

Jonas, Kristina; Tomenius, Henrik; Kader, Abdul; Normark, Staffan; Römling, Ute; Belova, Lyubov; Melefors, Öjar

doi:10.1186/1471-2180-7-70

Cited by 156 publications

(150 citation statements)

References 45 publications

(40 reference statements)

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“…This can be explained by the fact that the maximum expression of the major transcriptional regulator CsgD (curli subunit gene D, also described as agfD) under aerobic conditions occurs in a nutrient-limited medium (GERSTEL and RöMLING, 2001;STEPANoVIć et al, 2004). CsgD triggers the synthesis of two key biofilm matrix components, i.e., a protein component (curli fimbriae) and a polysaccharide (cellulose) (RöMLING, 2005;JoNAS et al, 2007). CsgD directly activates aggregative fimbriae genes (agf) encoding for the synthesis of curli fimbriae, and indirectly the genes bcsA, bcsB, bcsZ and bcsC (bacterial cellulose synthesis), encoding the synthesis of cellulose (RöMLING, 2005;JoNAS et al, 2007).…”

Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

“…CsgD triggers the synthesis of two key biofilm matrix components, i.e., a protein component (curli fimbriae) and a polysaccharide (cellulose) (RöMLING, 2005;JoNAS et al, 2007). CsgD directly activates aggregative fimbriae genes (agf) encoding for the synthesis of curli fimbriae, and indirectly the genes bcsA, bcsB, bcsZ and bcsC (bacterial cellulose synthesis), encoding the synthesis of cellulose (RöMLING, 2005;JoNAS et al, 2007). the stimulative effect of nutrient limitation on biofilm formation of S. Tennessee on a stainless steel surface was confirmed using scanning electron microscopy.…”

Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

“…The negative effect of higher temperature on biofilm production may be explained by the lack of synthesis of curli fimbriae, which in Salmonella commonly takes place at temperatures below 30 °C (SoLANo et al, 2002;GERSTEL and RöMLING, 2003). Curli fimbriae are extracellular protein polymers of vital importance for initial adhesion onto biotic and abiotic surfaces, and early cell aggregation (WhIte and Surette, 2006;JONaS et al, 2007). In the microplate biofilm assay, the isolates of S. tennessee did not form a visible biofilm on the bottom of the polystyrene plate wells; however, they formed a clearly visible pellicle in the liquid-air interface.…”

Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

“…the formation of a pellicle is characteristic of Salmonella species, signifying their preference for aerobic growth conditions (LAMAS et al, 2016). An important role in pellicle formation is attributed to BapA protein (biofilm associated protein a), a large secreted protein, which is loosely associated with the cell surface (LATASA et al, 2005;JONaS et al, 2007). Curli, cellulose and the cell surface protein BapA are the key components of the Salmonella biofilm matrix (JONaS et al, D.…”

Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

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Biofilm forming ability of Salmonella enterica serovar Tennessee isolates originating from feed

Milanov¹,

Prunić²,

Ljubojević³

2017

Vet. arhiv

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Salmonella enterica subspecies enterica serovar tennessee was the most frequent Salmonella serovar isolated from plant-based animal feed throughout a two-year period (2012)(2013) of research conducted in the region of the Autonomous Province of Vojvodina. In this study, the ability of biofilm formation was investigated on 20 isolates of S. Tennessee. The starting point for this research was the established biofilm-forming ability of serovar Tennessee strains, which significantly contributes to their wide distribution in the environment (plant materials), and hence in animal feed. In this study, biofilm formation was investigated on polystyrene (microplate biofilm assay) and stainless steel (scanning electron microscopy) surfaces by culturing in Luria Bertani broth (LB) and Triptone Soya Broth (TSB). The expression of major matrix components of Salmonella biofilm (curli fimbriae and cellulose) was examined by cultivation on Congo Red agar. All isolates of S. tennessee produced a biofilm on a polystyrene surface in a microtiter plate test, by cultivation in LB at an incubation temperature of 20 °C. Scanning electron microscopy revealed biofilm formation of S. tennessee on a stainless steel surface by cultivation in LB, but not in TSB. The expression of curli fimbriae and cellulose, as well as the formation of characteristic "rdar" colonies on Congo red agar has been observed in all isolates of S. tennessee. this serovar is not specifically adapted to humans and animals, so the available epidemiological data do not indicate its major implication in food-borne infection outbreaks. however, this serovar manifests a pronounced ability for adherence to plant surfaces, biofilm formation and increased resistance to long-term desiccation, which is the most likely explanation for its frequent identification in low water activity feed.

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Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

See 2 more Smart Citations

Biofilm forming ability of Salmonella enterica serovar Tennessee isolates originating from feed

Milanov¹,

Prunić²,

Ljubojević³

2017

Vet. arhiv

View full text Add to dashboard Cite

show abstract

“…The 13 DMC coded (DMC 7,12,20,23,40,41,57,58,70,71,75,76,77) Salmonella Infantis strains, isolated from CONTACT Mustafa Akçelik akcelik@science.ankara.edu.tr raw chicken samples collected from Turkey, were all obtained from Prokaryotes Genetics Laboratory, Biology Department, Ankara University. All isolates were stored at ¡80 C in a glycerine freezing medium and recovered in Luria Bertani (LB; Merck KGaA, Germany) at 37 C overnight.…”

Section: Salmonella Infantis Isolatesmentioning

confidence: 99%

Effects of sub-MIC antibiotic concentrations on biofilm production of Salmonella Infantis

Tezel

Akçelik

Yüksel

et al. 2016

Biotechnology & Biotechnological Equipment

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In the present study, 13 Salmonella Infantis strains, which have been originated from Turkey, were selected due to their clinical and industrial relevance, sufficient biofilm producing capability and multidrug resistance. Although all tested strains were built up of thin pellicle, optimum pellicle formation has occurred at 28 C. All S. Infantis biofilms were categorized as 'bdar' morphotype following the incubation at both 20 and 28 C, while they were categorized as 'saw' morphotype at 37 C. Under a certain incubation temperature (28 C), 84.62% of strains have formed strong biofilm structures. By using the disk diffusion method, high levels of resistance have been observed among tested bacteria against nalidixic acid (100%), spectinomycin (100%), streptomycin (92.3%), tetracycline (92.3%), kanamycin (76.9%) and neomycin (76.9%). Further studies were performed with S. Infantis DMC 12 strain, due to its capability to produce biofilm and multidrug resistance phenotype. Gentamycin (>64 mg/mL, 2 £ MIC) and tetracycline (>128 mg/mL, 4 £ MIC) were determined as the most effective antibiotics against biofilm formation. The biofilm forms have showed increased antimicrobial resistance when it was compared to the planktonic bacteria. The highest resistance rates of the biofilm bacteria were observed to neomycin (12 £ MIC) followed by spectinomycin (10 £ MIC) and streptomycin (10 £ MIC). Biofilm structure was induced as a result of nalidixic acid, spectinomycin, tetracycline and neomycin treatment at sub-MIC concentrations of tested antibiotics.

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Mechanisms of Plant Colonization by Human Pathogenic Bacteria: An Emphasis on the Roots and Rhizosphere

Holden

Pritchard

Wright

et al. 2013

Molecular Microbial Ecology of the Rhizosphere

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Roles of curli, cellulose and BapA in Salmonella biofilm morphology studied by atomic force microscopy

Cited by 156 publications

References 45 publications

Biofilm forming ability of Salmonella enterica serovar Tennessee isolates originating from feed

Biofilm forming ability of Salmonella enterica serovar Tennessee isolates originating from feed

Effects of sub-MIC antibiotic concentrations on biofilm production of Salmonella Infantis

Mechanisms of Plant Colonization by Human Pathogenic Bacteria: An Emphasis on the Roots and Rhizosphere

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