Roles of cell and microvillus deformation and receptor-ligand binding kinetics in cell rolling

Under increasing tensile load, the lifetime of a single catch bond counterintuitively increases up to a maximum and then decreases exponentially like a slip bond. So far, the characteristics of single catch bond dissociation have been extensively studied. However, it remains unclear how a cluster of catch bonds behaves under tensile load. We perform computational analysis on the following models to examine the characteristics of clustered catch bonds: (i) clusters of catch bonds with equal load sharing, (ii) clusters of catch bonds with linear load sharing, and (iii) clusters of catch bonds in micropipette-manipulated cell detachment. We focus on the differences between the slip and catch bond clusters, identifying the critical factors for exhibiting the characteristics of catch bond mechanism for the multiple-bond system. Our computation reveals that for a multiple-bond cluster, the catch bond behaviour could only manifest itself under relatively uniform loading conditions and at certain stages of decohesion, explaining the difficulties in observing the catch bond mechanism under real biological conditions.

show abstract

“…The values of related parameters in cases 1-3 were chosen based on existing studies [21,[45][46][47][48]…”

Section: System Parametersmentioning

confidence: 99%

Effect of loading conditions on the dissociation behaviour of catch bond clusters

Sun

Cheng

Gao

et al. 2011

J. R. Soc. Interface.

View full text Add to dashboard Cite

show abstract

“…The membrane kinematics are imposed as appropriately flow advected Lagrangian surface mesh points, and the membrane dynamics are computed through structural mechanics modelling subject to interpolated flow pressure and viscous stress. Many workers have pursued the IBM and hybrid IBM methods for RBC flow simulation [16,17,[35][36][37][38][39][40][41]. Closely related VOF methods have been applied to WBC adhesion [42].…”

Section: Fluid Dynamicsmentioning

confidence: 99%

Multi-scale biological and physical modelling of the tumour micro-environment

Kunz

Gaskin

et al. 2015

Drug Discovery Today: Disease Models

View full text Add to dashboard Cite

Paced by advances in high performance computing, and algorithms for multi-physics and multi-scale simulation, a number of groups have recently established numerical models of flowing blood systems, where cellscale interactions are explicitly resolved. To be biologically representative, these models account for some or all of: (1) fluid dynamics of the carrier flow, (2) structural dynamics of the cells and vessel walls, (3) interaction and transport biochemistry, and, (4) methods for scaling to physiologically representative numbers of cells. In this article, our interest is the modelling of the tumour micro-environment. We review the broader area of cell-scale resolving blood flow modelling, while focusing on the particular interactions of tumour cells and white blood cells, known to play an important role in metastasis.

show abstract

“…105 Pawar et al have modeled cell body and microvillus deformation and predicted that both catch-slip-bond behavior and lesser cell deformation are responsible for threshold phenomenon observed on selectin-mediated leukocyte rolling over ligand-immobilized substrate under shear flow. 76 …”

Section: Mathematical Modeling On Receptor-ligand Binding In Cell Adhmentioning

confidence: 99%

Advances in Experiments and Modeling in Micro- and Nano-Biomechanics: A Mini Review

et al. 2011

View full text Add to dashboard Cite

Abstract-Recent advances in micro-and nano-technologies and high-end computing have enabled the development of new experimental and modeling approaches to study biomechanics at the micro-and nano-scales that were previously not possible. These new cutting-edge approaches are contributing toward our understanding in emerging areas such as mechanobiology and mechanochemistry. Another important potential contribution lies in translational medicine, since biomechanical studies at the cellular and molecular levels have direct relevance in areas such disease diagnosis, nano-medicine and drug delivery. Thus, the developed experimental and modeling approaches are critical in elucidating important mechanistic insights in both basic sciences and clinical treatment. While it is hard to cover all the recent advances in this mini-review, we focus on several important approaches. For experimental techniques, we review the assays involving shear flow, cellular imaging, microbead, microcontact printing, and micropillars at the micro-scale, and micropipette aspiration, optical tweezers, parallel flow chamber, and atomic force microscopy at the nano-scale. In modeling and simulations, we outline the theoretical modeling for actin dynamics in migrating cell and actin-based cell motility in cellular mechanics, as well as the receptor-ligand binding in cell adhesion and the application of free, steered, and flow molecular dynamics simulations in molecular biomechanics. Relevant scientific issues and applications are also discussed.

show abstract

Roles of cell and microvillus deformation and receptor-ligand binding kinetics in cell rolling

Cited by 40 publications

References 48 publications

Effect of loading conditions on the dissociation behaviour of catch bond clusters

Effect of loading conditions on the dissociation behaviour of catch bond clusters

Multi-scale biological and physical modelling of the tumour micro-environment

Advances in Experiments and Modeling in Micro- and Nano-Biomechanics: A Mini Review

Contact Info

Product

Resources

About