Roles of bacterial membrane vesicles

Ávila-Calderón, Eric Daniel; Araiza-Villanueva, Minerva Georgina; Cancino-Diaz, Mario E.; López-Villegas, Edgar Óliver; Sriranganathan, Nammalwar; Boyle, Stephen M.; Contreras-Rodríguez, Araceli

doi:10.1007/s00203-014-1042-7

Cited by 70 publications

(61 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exosomes are used to communicate between cells, and have been engineered to incorporate heterologous cargoes (Marcus and Leonard 2013). Similarly, in Gram-negative bacteria, outer membrane vesicles are produced naturally for many purposes, including carrying toxins and protecting bacteria from antibiotics and phage (reviewed in Kulp and Kuehn 2010;Avila-Calderó n et al 2015). They are also the focus of active engineering efforts, for example, in the production of vaccines ).…”

Section: The Extracellular Environmentmentioning

confidence: 99%

Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities

Polka

Hays

Silver

2016

Cold Spring Harb Perspect Biol

View full text Add to dashboard Cite

Traditional views of synthetic biology often treat the cell as an unstructured container in which biological reactions proceed uniformly. In reality, the organization of biological molecules has profound effects on cellular function: not only metabolic, but also physical and mechanical. Here, we discuss a variety of perturbations available to biologists in controlling protein, nucleotide, and membrane localization. These range from simple tags, fusions, and scaffolds to heterologous expression of compartments and other structures that confer unique physical properties to cells. Next, we relate these principles to those guiding the spatial environments outside of cells such as the extracellular matrix. Finally, we discuss new directions in building intercellular organizations to create novel symbioses.

show abstract

Section: The Extracellular Environmentmentioning

confidence: 99%

Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities

Polka

Hays

Silver

2016

Cold Spring Harb Perspect Biol

View full text Add to dashboard Cite

show abstract

“…toxins and proteases), DNA to enable horizontal gene transfer or signaling molecules for intra-and inter-species communication (e.g. quorum sensing signaling molecules) [3,4]. OMVs range from 10-300 nm in diameter and consist mainly of OM components, such as phospholipids, OM proteins, and lipooligosaccharide (LOS) or lipopolysaccharide (LPS).…”

Section: Descriptionmentioning

confidence: 99%

A Glimpse on Outer Membrane Vesicles as Vaccine Candidates

Rl¹,

Gz²,

Schild³

2015

J Vaccines Vaccin

View full text Add to dashboard Cite

DescriptionRecently it becomes increasingly evident that actively release of membrane-derived vesicles from cellular surfaces is conserved in all domains of life, including eukaryotes, archaea, Gram-positive and Gram-negative bacteria. For the latter case these spherical structures originate from the outer membrane (OM) and are consequently referred to as outer membrane vesicles (OMVs). Notably, all Gramnegative bacteria that have been investigated so far are able to naturally release OMVs [1,2]. Although OMV formation seems to be a common feature of Gram-negative bacteria the knowledge of their biological roles and biogenesis remains very limited. The hypothesized functions range from simple waste dumps to delivery vehicles for virulence factors (e.g. toxins), degradation enzymes (e.g. toxins and proteases), DNA to enable horizontal gene transfer or signaling molecules for intra-and inter-species communication (e.g. quorum sensing signaling molecules) [3,4]. OMVs range from 10-300 nm in diameter and consist mainly of OM components, such as phospholipids, OM proteins, and lipooligosaccharide (LOS) or lipopolysaccharide (LPS). Additionally, OMVs contain periplasmic components, which are trapped in the lumen of OMVs during the vesiculation process [5]. Hence, OMVs are basically non-living facsimiles of the bacterial cell surface, naturally containing multiple native surface-exposed antigens as well as immunostimulatory molecules [1,6]. Based on their aforementioned immunogenic potency and on positive examples of the OMV-derived vaccines against serogroup B Neisseria meningitides, we initiated several projects over the last years to analyze the potential of OMVs derived from human Gram-negative pathogens as vaccine candidates [7][8][9][10][11][12]. This concise communication summarizes our recent findings in the emerging research field of OMVs as vaccine candidates including immunization studies against human gastrointestinal pathogens (e.g. V. cholerae and ETEC) as well as human and veterinarian respiratory tract pathogens (e.g. Pasteurellaceae family members).For example, we comprehensively characterized OMVs derived from V. cholerae as a new approach for an effective vaccine candidate against cholera [7][8][9][10]13]. As it turns out OMVs exhibit several advantages compared to other vaccines. For example, OMVs are highly stable, even at room temperature, and highly immunogenic without requirements of additional adjuvants [7][8][9]. Thus, a cold chain or accessory buffer solutions are unlikely to be required for the OMV vaccine candidate. In addition, OMV donor strains can be genetically modified to secrete altered OMVs. We successfully applied this strategy to isolate OMVs from a LPS acyltransferase mutant strain, demonstrating that reduction of OMV endotoxicity can be achieved without diminishing the immunogenic potential by genetic modification resulting in underacylated lipid A [10]. Overall, we demonstrated the induction of a specific, long-lasting, high-titer, protective immune response upon mucosal immuniz...

show abstract

“…The study of the EVs was initially limited to Gram-negative bacteria, as it was thought that the rigidity of the Gram-positive cell wall, which is rich in peptidoglycans, would not allow vesicle blebbing [11]. However, the production of EVs was also observed in some Gram-positive bacteria [14–17]. Current studies [18–20] showed that the activity of cell wall-degrading enzymes, which weaken the peptidoglycan layer and thus facilitate the release of Gram-positive EVs, could probably explain such phenomena in Gram-positive bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…Several studies [13, 14, 18] showed that bacteria exploit vesicle production as a virulence strategy. Bacterial components, including virulence factors, are packed in the vesicles and delivered to the host cells and tissues.…”

Section: Introductionmentioning

confidence: 99%

Proteomics of Extracellular Vesicles Produced byGranulicatellaSpecies that cause Infective Endocarditis

Ellepola

et al. 2019

Preprint

View full text Add to dashboard Cite

30When oral bacteria accidentally enter the bloodstream due to transient tissue 31 damage during dental procedures, they have the potential to attach to the endocardium 32 or an equivalent surface of an indwelling prosthesis and cause infection. Many bacterial 33 species produce extracellular vesicles (EVs) as part of normal physiology, but also use 34 it as a virulence strategy. In this study, it was hypothesized that Granulicatella species 35 produce EVs that possibly help them in virulence. Therefore, the objectives were to 36 isolate and characterize EVs produced by these species and to investigate their immune-37 stimulatory effects. The reference strains G. adiacens CCUG 27809 and G. elegans 38 CCUG 38949 were cultured on chocolate blood agar for 2 days. From subsequent broth 39 cultures, the EVs were isolated using differential centrifugation and filtration protocol 40 and then observed using scanning electron microscopy. Proteins in the vesicle 41 preparations were identified by nano LC-ESI-MS/MS. The EVs proteomes were 42 analyzed and characterized using different bioinformatics tools. The immune-43 stimulatory effect of the EVs was studied via ELISA quantification of IL-8, IL-1β and 44 CCL5, major proinflammatory cytokines, produced from stimulated human PBMCs. It 45 was revealed that both G. adiacens and G. elegans produced EVs, ranging in diameter 46 from 30 to 250 nm. Overall, G. adiacens EVs contained 160 proteins, and G. elegans 47 EVs contained 107 proteins. Both proteomes consist of several ribosomal proteins, 48DNA associated proteins, binding proteins, and metabolic enzymes. It was also shown 49 that these EVs carry putative virulence factors including moonlighting proteins. These 50 EVs were able to induce the production of IL-8, IL-1β and CCL5 from human PBMCs. 51The diversity in EVs content indicates that these vesicles could have possible roles in 3 52 bacterial survival, invasion, host immune modulation as well as infection. Further 53 functional characterization of the Granulicatella EVs may provide new insights into 54 virulence mechanisms of these important but less studied oral bacterial species. 55 56 57 Granulicatella species, formerly known as nutritionally variant streptococci 58 based on their characteristic dependence on pyridoxal or cysteine supplementation for 59 their growth in standard media [1], are catalase and oxidase negative, non-motile, non-60 spore-forming, facultatively anaerobic Gram-positive cocci [2, 3]. They are part of the 61 normal oral flora [4], but cause serious infections such as infective endocarditis. The 62 genus Granulicatella consists of 3 species: Granulicatella adiacens, Granulicatella 63 elegans and Granulicatella balaenopterae [3]. The species G. balaenopterae has not 64 been isolated from human samples, whereas both G. adiacens and G. elegans have been 65 reported from IE cases [5, 6]. In addition, these oral commensal cocci have been 66 associated with endodontic infections [7, 8], dental caries [9], and periodontitis [8, 10] 67 via DNA-based s...

show abstract

Roles of bacterial membrane vesicles

Cited by 70 publications

References 90 publications

Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities

Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities

A Glimpse on Outer Membrane Vesicles as Vaccine Candidates

Proteomics of Extracellular Vesicles Produced byGranulicatellaSpecies that cause Infective Endocarditis

Contact Info

Product

Resources

About