Roles of AKT and sphingosine kinase in the antiapoptotic effects of bile duct ligation in mouse liver

Osawa, Yosuke; Hannun, Yusuf A.; Proia, Richard L.; Brenner, David A.

doi:10.1002/hep.20967

Cited by 43 publications

(51 citation statements)

References 29 publications

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“…18 Blots were incubated with antibodies against phosphoFoxO1 (Ser-256), phospho-AKT (Ser-473), hemagglutinin (both Cell Signaling, Beverly, MA), FoxO1, AKT (both Santa Cruz, Santa Cruz, CA), p27 kip1 (BD Biosciences), MnSOD (Stressgen, Victoria BC, Canada), ␣-SMA, ␤-actin (both Sigma-Aldrich), and desmin (DAKO, Glostrup, Denmark) overnight at 4°C. For detection of insulin receptor substrate-2 (IRS-2) tyrosine phosphorylation, cell lysates from insulin-treated rat HSCs were immunoprecipitated with anti-IRS-2 antibody (Upstate, Lake Placid, NY) and protein A-agarose (Santa Cruz).…”

Section: Western Blot Analysis and Immunoprecipitationmentioning

confidence: 99%

The Forkhead Transcription Factor FoxO1 Regulates Proliferation and Transdifferentiation of Hepatic Stellate Cells

et al. 2007

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Section: Western Blot Analysis and Immunoprecipitationmentioning

confidence: 99%

The Forkhead Transcription Factor FoxO1 Regulates Proliferation and Transdifferentiation of Hepatic Stellate Cells

et al. 2007

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“…Internal signaling pathways such as S1P/SPK, ERK, and Stat3 play a pivotal role in the process of hepatocyte proliferation. 1,2,15,21,22 We investigated the effect of rhHPPCn on SPK, ERK1/2, and Stat3 in SMMC7721 cells. rhHPPCn treatment caused a rapid increase in SPK activity (Fig.…”

Section: Effect Of Rhhppcn On Cell Growth and Proliferation In Vitromentioning

confidence: 99%

Isolation and functional identification of a novel human hepatic growth factor: Hepatopoietin Cn

Chu¹,

Zhang²,

Shi³

et al. 2008

Hepatology

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Hepatic stimulating substance (HSS) was first isolated from weanling rat liver in 1975 and found to stimulate hepatic DNA synthesis both in vitro and in vivo. Since then, mammalian and human HSS have been investigated for their potential to treat hepatic diseases. However, the essential nature in composition and structure of HSS remain puzzling because HSS has not been completely purified. Heating, ethanol precipitation, and ion-exchange chromatographies had been carried out to isolate the protein with specific stimulating activity from newborn calf liver, and [ 3 H]thymidine deoxyribose (TdR)/bromodeoxyuridine (BrdU) incorporation and carboxyfluorescein diacetate succinimidyl ester (CFSE)-based proliferation assay to determine the bioactivity in vitro and in vivo. We report the purification of a novel 30-kDa protein from a crude extract of calf liver HSS. This protein is a member of the leucine-rich acidic nuclear protein family (LANP) and has been named hepatopoietin Cn (HPPCn). Studies of partially hepatectomized (PH) mice show that levels of HPPCn messenger RNA (mRNA) increase after liver injury. Furthermore, the recombinant human protein (rhHPPCn) was shown to stimulate hepatic DNA synthesis and activate signaling pathways involved in hepatocyte proliferation in vitro and in vivo. Conclusion: HPPCn is a novel hepatic growth factor that plays a role in liver regeneration. (HEPATOLOGY 2008;47:986-995.)

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“…AKT and Erk1/2 signaling pathways are known to play a key role in cell survival and proliferation, respectively. 34,35 Therefore, ILK could play a role in sustaining the proliferation of the activated HSCs, thus preventing their death by apoptosis. 34 To test this hypothesis we transfected CFSC-2G with ILK siRNA and determined the extent of PKB and MAPKs phosphorylation and HSC proliferation.…”

Section: Mmp-9mentioning

confidence: 99%

Involvement of integrin-linked kinase in carbon tetrachloride–induced hepatic fibrosis in rats

et al. 2006

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Integrin-linked kinase (ILK) is a multidomain focal adhesion protein implicated in signal transduction between integrins and growth factor receptors. Although its expression is upregulated in pulmonary and renal fibrosis, its role in the development of hepatic fibrosis remains to be determined. Therefore, we considered it important to investigate whether ILK is involved in activation of hepatic stellate cells and thus plays a role in the development of hepatic fibrosis. Immunohistochemical analysis of liver sections obtained from rats with CCl 4 -induced cirrhosis revealed increased expression and colocalization of ILK and alpha-smooth muscle actin in hepatic stellate cells in perisinusoidal areas. In addition, hepatic stellate cells isolated from fibrotic livers expressed high levels of ILK and alpha-smooth muscle actin, and their expression was sustained in culture. In contrast, hepatic stellate cells (HSCs) isolated from normal rat liver did not express ILK, but its expression was increased when the cells were activated in culture. Our studies also showed that ILK is involved in the phosphorylation of ERK 1/2, p38 MAPK, JNK, and PKB and that selective inhibition of ILK expression by siRNA results in a significant decrease in their phosphorylation. These changes were accompanied by significant inhibition of cell spreading and migration without affecting cell proliferation. In conclusion, ILK plays a key role in HSC activation and could be a possible target for antifibrogenic therapy. (HEPATOLOGY 2006;44:612-622.)

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Roles of AKT and sphingosine kinase in the antiapoptotic effects of bile duct ligation in mouse liver

Cited by 43 publications

References 29 publications

The Forkhead Transcription Factor FoxO1 Regulates Proliferation and Transdifferentiation of Hepatic Stellate Cells

The Forkhead Transcription Factor FoxO1 Regulates Proliferation and Transdifferentiation of Hepatic Stellate Cells

Isolation and functional identification of a novel human hepatic growth factor: Hepatopoietin Cn

Involvement of integrin-linked kinase in carbon tetrachloride–induced hepatic fibrosis in rats

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