Roles for Sphingolipids in Saccharomyces cerevisiae

Dickson, Robert C.

doi:10.1007/978-1-4419-6741-1_15

Cited by 93 publications

(94 citation statements)

References 135 publications

(187 reference statements)

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“…In addition to glycerophospholipids, yeast cells contain a second class of phospholipids termed sphingolipids (Schneiter 1999;Guan and Wenk 2006;Dickson 2010). These lipids are derived from sphingosine or phytosphingosine, which are produced in a condensation reaction between serine and palmitoyl-CoA.…”

Section: Sphingolipidsmentioning

confidence: 99%

Analyzing and Understanding Lipids of Yeast: A Challenging Endeavor

Kohlwein

2017

Cold Spring Harb Protoc

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Lipids are essential biomolecules with diverse biological functions, ranging from building blocks for all biological membranes to energy substrates, signaling molecules, and protein modifiers. Despite advances in lipid analytics by mass spectrometry, the extraction and quantitative analysis of the diverse classes of lipids are still an experimental challenge. Yeast is a model organism that provides several advantages for studying lipid metabolism, because most biosynthetic pathways are well described and a great deal of information is available on the regulatory mechanisms that control lipid homeostasis. In addition, the composition of yeast lipids is much less complex than that of mammalian lipids, making yeast an excellent reference system for studying lipid-associated cell functions.

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Section: Sphingolipidsmentioning

confidence: 99%

Analyzing and Understanding Lipids of Yeast: A Challenging Endeavor

Kohlwein

2017

Cold Spring Harb Protoc

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“…Furthermore, lipids can be distributed asymmetrically across the leaflets of a membrane bilayer (67). Thus, the concerted synthesis, degradation, modification, and trafficking of lipids generate and sustain the highly heterogeneous lipid composition of organellar membranes (68)(69)(70). Mitochondria are organelles of endosymbiotic origin that, over evolution, have transferred most of their protein encoding genes into the cell host nucleus, with only a few genes still residing in the mitochondrial DNA (mtDNA) (71).…”

Section: Making Mitochondrial Membranes: the Contribution Of Er-mitocmentioning

confidence: 99%

Lipid synthesis and membrane contact sites: a crossroads for cellular physiology

Fernández-Murray

McMaster

2016

Journal of Lipid Research

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high throughput analyses, makes this yeast a powerful organism to unveil the intricacies of this field. The yeast S. cerevisiae has played a foundational role in the field of molecular and cellular biology of lipids, including the identification of membrane contact sites (MCSs) and how they link lipid metabolism with organelle dynamics. We present advances in two topics where extensive and outstanding contributions have recently been made where their relevance transcends the yeast lipid field. We first review new information about the phosphatidic acid (PA) phosphatase, Pah1, the yeast homolog of human lipin, and its role in directing lipid flux into membrane synthesis or storage. We review the contribution of Pah1 to lipid droplet (LD) formation and its interaction with the seipin complex, Fld1/Ldb16, to regulate endoplasmic reticulum (ER)-LD contact site dynamics. Second, we recapitulate recent developments revealing MCS connections between the ER and mitochondria with other cellular compartments, and how these intersect with the maintenance of lipid homeostasis. Furthermore, we explore MCS redundancies that allow cells to cope with changing metabolic demand. PA METABOLISM CO-COORDINATES MEMBRANE AND LD SYNTHESISPA is the common precursor for the synthesis of membrane phospholipids (PLs) and the major component of The pervasive importance of lipids in cell biology has become evident. From structural roles defining cellular compartments and surfaces with specific biological properties to functional roles in signal transduction processes and modulation of regulatory networks, the involvement of lipids in varied cellular functions is well-established. Concurrently, we have gained a broad understanding about the repertoire of proteins involved in synthesis, degradation, transport, and sensing of lipids, as well as the regulatory mechanisms that interconnect lipid metabolism with other cellular processes. The amenability of Saccharomyces cerevisiae to classical approaches of molecular genetics, and toThe work on the science described in this review was supported by a Discovery Grant from the Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada to C.R.M. Manuscript received 19 July 2016 and in revised form 6 August 2016. Published, JLR Papers in Press, August 12, 2016 DOI 10.1194 Lipid synthesis and membrane contact sites: a crossroads for cellular physiology Abbreviations: cER, cortical endoplasmic reticulum; ChiMERA, construct helping in mitochondria-endoplasmic reticulum association; CL, cardiolipin; DAG, diacylglycerol; EMC, endoplasmic reticulum membrane protein complex; ER, endoplasmic reticulum; ERMES, endoplasmic reticulum-mitochondria encounter structure; HOPS, homotypic fusion and vacuole protein sorting; Lam, lipid transfer protein anchored at a membrane contact site; LD, lipid droplet; Ltc, lipid transfer at contact site; LTP, lipid transfer protein; Mcp, maintenance of mitochondrial morphology 10 complementing protein; M...

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“…Their abundance in the model yeast S. cerevisiae, representing 30% of phospholipid from the plasma membrane [32], eased investigation of their structure, metabolism and function. These sphingolipids are key signaling molecules in a number of yeast cellular processes including trafficking/exocytosis, endocytosis, actin cytoskeleton dynamics, membrane microdomains, calcium signaling, cell cycle control, stress resistance and nutrient uptake (see reviews [33][34][35]). …”

Section: Inositolphosphoceramidementioning

confidence: 99%

Sphingolipids from the human fungal pathogen Aspergillus fumigatus

Fontaine

2017

Biochimie

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Roles for Sphingolipids in Saccharomyces cerevisiae

Cited by 93 publications

References 135 publications

Analyzing and Understanding Lipids of Yeast: A Challenging Endeavor

Analyzing and Understanding Lipids of Yeast: A Challenging Endeavor

Lipid synthesis and membrane contact sites: a crossroads for cellular physiology

Sphingolipids from the human fungal pathogen Aspergillus fumigatus

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