Roles for CCN2 in normal physiological processes

Hall‐Glenn, Faith; Lyons, Karen M.

doi:10.1007/s00018-011-0782-7

Cited by 102 publications

(88 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From early disc development to maturation, many studies have reported an anabolic effect of CCN2 in maintaining the matrix components of the disc (Chiou et al, 2006;Hall-Glenn and Lyons, 2011;Huang et al, 2010;Tran et al, 2013a;Tran et al, 2013b;Tran et al, 2011). In the degenerative disc, several recent studies have demonstrated a positive correlation between CCN2 levels and the severity of degeneration (Tran et al, 2010) and the presence of pain (Peng et al, 2009).…”

Section: Different Molecular Signals and Interactions In Normal Intermentioning

confidence: 99%

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Aker¹,

Ghannam²,

Alzuabi³

et al. 2017

The Spine Scholar

View full text Add to dashboard Cite

The unique properties of the intervertebral disc (IVD) are evident in its structural complexity and functional importance for spinal support and stability. The contributions of the different cellular and extracellular components to the function of the IVD depend on their distinctive molecular features and pathways. Disruption of these molecular pathways influences the pathological changes involved in IVD degeneration. Therefore, the molecular features of the IVD have been the focus of interest for many researchers seeking to elucidate its normal functioning, potential pathologies, and appropriate therapies. The aim of the present article is to review the molecular aspects of IVD development, specific cellular markers, and the interactions between cellular and extracellular components responsible for homeostasis, degeneration and potential therapies. The literature available via PubMed and Google Scholar was reviewed and the relevant references in review articles were searched manually. Spine

show abstract

Section: Different Molecular Signals and Interactions In Normal Intermentioning

confidence: 99%

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Aker¹,

Ghannam²,

Alzuabi³

et al. 2017

The Spine Scholar

View full text Add to dashboard Cite

show abstract

“…Connective tissue growth factor (CTGF, CCN2) belongs to the CCN (an acronym of Cyr61/CEF-10, CTGF/Fisp-12, Nov) family of immediate early genes, which are highly conserved among different species. Studies have shown that CTGF has essential roles in the development of normal tissues, such as follicle, ovulation [2], chondrogenesis, cartilage [3], alveolarization during lung maturation [4], and angiogenesis [5]. In addition, CTGF has crucial roles in epithelial tissues [6,7].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional and posttranscriptional regulation of CXCL8/IL-8 gene expression induced by connective tissue growth factor

et al. 2015

View full text Add to dashboard Cite

Connective tissue growth factor (CTGF), a CCN family member, is a secreted protein regulating cellular functions, including fibrosis, apoptosis, adhesion, migration, differentiation, proliferation, angiogenesis, and chondrogenesis. CTGF increases proinflammatory factor production; however, inflammatory cytokine regulation by CTGF is poorly understood. The aim of this study was to identify novel biological functions and elucidate the functional mechanisms of CTGF. Specifically, the study focused on the ability of CTGF-primed monocytes to secrete interleukin 8 (CXCL8/IL-8) and determined the signaling pathways involved in CTGF-induced CXCL8/IL-8 gene regulation during inflammation. We transfected wild-type or mutant CXCL8/IL-8 promoter-derived luciferase reporter constructs into 293T cells to examine the effect of CTGF on the CXCL8/IL-8 promoter. The results showed that the activator protein-1 and nuclear factor κB binding sites of the CXCL8/IL-8 promoter are essential for CTGF-induced CXCL8/IL-8 transcription. Moreover, the CTGF-induced activation of p38 mitogen-activated protein kinase (MAPK), c-Jun-N-terminal kinase, and extracellular signal-regulated kinase (ERK) is involved in this process. In addition, adenosine-uridine-rich elements (AREs) of the CXCL8/IL-8 3'-untranslated region (3'-UTR) reduce CXCL8/IL-8 mRNA stability. To investigate whether CTGF regulates CXCL8/IL-8 gene expression at the posttranscriptional level, we transfected 293 cells with serial luciferase constructs containing different segments of the CXCL8/IL-8 3'-UTR and then stimulated the cells with CTGF. The results suggested that CTGF stabilized luciferase mRNA and increased luciferase activity by regulating the CXCL8/IL-8 3'-UTR. Moreover, the p38 MAPK pathway may contribute to CTGF-induced CXCL8/IL-8 mRNA stabilization.

show abstract

“…Sustained hyperglycemia can promote the release of some cytokines and growth factors such as connective tissue growth factor (CTGF) and epidermal growth factor-β (TGF-β) [14]. The massive relaxation of CTGF in the course of long-term diabetes will cause a huge expression of extracellular matrix(ECM) [15][16], which can promote cell hypertrophy and interstitial fibrosis, leading to the change of cardiac functions. Even more, TGF-β can also induce cell hypertrophy and mesangial matrix expansion through a series signaling reactions with receptors [17].…”

Section: Introductionmentioning

confidence: 99%

An Inhibition Effect on Diabetic Cardiomyopathy Fibrosis of db/db Mice through Reduction of Myocardial Protein N-glycosylation by Crude 1-DNJ Extract from Homemade <em>Bombyx Batryticatus</em> mori.L

Zhao¹,

Tz²,

Qc³

et al. 2018

Preprint

View full text Add to dashboard Cite

The Chinese drug Bombyx Batryticatus mori.L which also named as the white stiff silkworm is widely used in clinics, due to the significant antispasmodic and promotional blood circulation effects. In addition, its hypoglycemic effect is also recognized in recent years. From a pathological point of view, the enzymatic glycosylation and non-enzymatic glycation both have important roles in regulating properties of proteins and are associated with Diabetes. With the db/db mouse model, we examined the alterations of N-glycosylation of diabetic myocardium at primary stage and clarify the differences in glycosylation of myocardium before and after with 1-DNJ treatment. Hydrophilic chromatography solid phase extraction enrichment and LC-MS/MS identification was applied to profile the alternations in protein glycosylation. Meanwhile, N-glycan α1, 6-fucosylation alterations were profiled with LCA lectin blot and FITC-labelled lectin affinity histochemistry. Our results showed that AGES, hydroxyproline, CTGF and other serum indicators and fibrosis related cytokines expressional levels were reduced significantly by 1-DNJ in a dose-dependent manner. In order to verify this result, the well-known pathway of TGF-β/smad2/3 was picked out and α1, 6-core fucosylated TGFR-β Ⅱ was semi-quantified with western blot method. The result sustained the conclusion from LCA lectin affinity histochemistry and lectin blot analysis. The expressional level of α1, 6-fucosyltransferase mRNA was increased in the myocardium of db/db mice, however, the 1-DNJ administration did not show obvious inhibitory effect on FU8expression. This unexpected result can be interpreted as 1-DNJ plays the roles by reducing the concentration of substrate rather than inhibiting α1 ， 6-fucose glycosyltransferase expression. Meanwhile, 1-DNJ crude extract from BBm with some flavonoids accompany can also play the roles of anti-oxidant, and all the chemicals protect the diabetic myocardium from hyperglycemia damage commonly.

show abstract

Roles for CCN2 in normal physiological processes

Cited by 102 publications

References 74 publications

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Transcriptional and posttranscriptional regulation of CXCL8/IL-8 gene expression induced by connective tissue growth factor

An Inhibition Effect on Diabetic Cardiomyopathy Fibrosis of db/db Mice through Reduction of Myocardial Protein N-glycosylation by Crude 1-DNJ Extract from Homemade <em>Bombyx Batryticatus</em> mori.L

Contact Info

Product

Resources

About