Role of β3-adrenoceptors for intrahepatic resistance and portal hypertension in liver cirrhosis

Trebicka, Jonel; Hennenberg, Martin; Pröbsting, Andrea Schulze; Laleman, Wim; Klein, Sabine; Granzow, M; Nevens, Frederik; Zaagsma, Johan; Heller, J.; Sauerbruch, Tilman

doi:10.1002/hep.23222

Cited by 61 publications

(86 citation statements)

References 51 publications

(99 reference statements)

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“…The slight difference may be attributed either to experimental techniques and/or species differences. Recently, Trebicka et al (2009) detected the presence of ADRB3 in the liver of humans and rats, and found that ADRB3 was involved in portal hypertension in liver cirrhosis.…”

Section: Discussionmentioning

confidence: 99%

Distribution and quantification of β-3 adrenergic receptor in tissues of sheep

Liu

et al. 2011

Animal

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The b-3 adrenergic receptor (ADRB3) is a G-protein coupled receptor involved in regulating lipolysis, as part of homeostatic regulation. In this study, South African Mutton Merino and Shanxi Dam Line were used to study the distribution and quantification of ADRB3 in adipose (subcutaneous, omental, retroperitoneal, mesenteric and perirenal fat) and non-adipose (heart, liver, spleen, lung and kidney) tissues of sheep. The protein was determined by immunohistochemical technique and by mRNA abundance via real-time polymerase chain reaction. ADRB3 was detected in all studied tissues with abundance in adipose tissues higher than in non-adipose tissues ( P , 0.001). For adipose tissues, greater expression was found in deep deposits such as great omental and retroperitoneal fat than in subcutaneous fat ( P , 0.05). Significant differences ( P , 0.05) both for mRNA and for protein expression also existed between the two sheep flocks. These findings are consistent with the known function of ADRB3 in mediating lipolysis and homeostasis in adipose tissues.Keywords: b-3 adrenergic receptor, sheep, tissues, mRNA expression, immunohistochemistry ImplicationsIn this study, the b-3 adrenergic receptor (ADRB3) expression in various tissues of sheep was detected by immunohistochemical technique and by mRNA abundance via real-time PCR. Despite its low expression, the presence of ADRB3 in the spleen and kidney was found in livestock for the first time. Generally, ADRB3 expressed higher in adipose than in non-adipose tissues, and for the former, it was higher in deep fat deposits than in subcutaneous fat. The distributions are consistent with the known functions of ADRB3 in mediating lipolysis and homeostasis in adipose tissues, and relaxation of vascular smooth muscle in the cardiovascular system. The relation between low expression and its function in some viscera, for example, lung, spleen and kidney of sheep deserves further study.Introduction b-Adrenergic receptors (ADRBs) are G-protein coupled receptors that mediate effects of the endogenous catecholamines adrenaline and noradrenaline. Three subtypes of ADRBs have been identified, named ADRB1, ADRB2 and ADRB3, respectively. These receptors are present in most mammalian tissues, but the distribution of each subtype varies among tissues in a given species. Among the various physiological roles of ADRBs, the ADRB3 subtype mediates lipolysis and stimulates thermogenesis in adipose tissues (Cannon and Nedergaard, 2004) by activating uncoupling protein in mitochondria.In early studies, ADRB3 was identified mainly on the surface of white and brown adipocytes as reviewed by Strosberg (1997). In recent years, expression of the protein was also found in the human heart (Rozec and Gauthier , (Rodriguez et al., 1995).It has been confirmed that the mRNA of the ADRB3 gene is expressed in various tissues of domestic animals. The 2.0 kb ADRB3 transcript in the bovine brown adipose tissue (PietriRouxel et al., 1995), and two transcripts of 2.2 and 1.9 kb in porcine subcutaneous fat (McNeel and ...

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Section: Discussionmentioning

confidence: 99%

Distribution and quantification of β-3 adrenergic receptor in tissues of sheep

Liu

et al. 2011

Animal

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“…Additional agents with impact on HSC activation and contractility include mitochondrial antioxidants [95,96], renin-angiotensin system inhibitors [97], and relaxin [98]. Finally, selective Experimental Intestinal decontamination by rifaximin blocks TLR4-mediated activation of HSCs and modulates FXR signaling by changing intestinal bile acid composition associated with reduced fibrosis, angiogenesis, and PVP in experimental PHT [69] beta3 receptor agonists induce relaxation of HSCs via cAMP accumulation and Rho-kinase inhibition with no effect on normal PVP, indicating that these drugs primarily target the dynamic components of IHVR [99,100]. FXR has recently become a major pharmacological target in NAFLD owing to its complex role in bile acid and lipid metabolism, inflammation, and fibrosis [101].…”

Section: Therapeutic Perspectivesmentioning

confidence: 99%

Origins of Portal Hypertension in Nonalcoholic Fatty Liver Disease

Baffy

2018

Dig Dis Sci

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“…Hemodynamic studies were performed under ketamine/xylazine anesthesia (78mg/kg / 12.5mg/kg i.p.) as previously described [14][15][16][17]. Rats were fasted overnight but allowed free access to water.…”

Section: Animalsmentioning

confidence: 99%

“…Hemodynamics were investigated using the coloured microsphere technique as previously described [14][15][16][17]. The colored microsphere technique was validated by the more frequently used radioactive microsphere method [18].…”

Section: Animalsmentioning

confidence: 99%

“…It has the advantage of being non-radioactive and using different colors at the same organism. This technique has been shown very accurate in the measurement of hemodynamics in many studies of our group and others [14][15][16][18][19][20][21][22][23]. A reference sample was obtained for 1 min at a rate of 0.65mL/min, using a continuous withdrawal pump (Hugo-Sachs-Elektronik,March-Hugstetten,Germany).…”

Section: Animalsmentioning

confidence: 99%

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