Role of β-glucuronidase, a lysosomal enzyme, in the pathogenesis of intestinal amoebiasis: an experimental study

Virk, K. J.; Mahajan, Rohit; Dilawari, J. B.; Ganguly, N. K.

doi:10.1016/0035-9203(88)90149-6

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…The effects in vivo of the altered lysosomal enzyme release in jaundice are not yet clear. It is known that monocyte lysosomal enzyme may cause tissue damage in amoebiasis of the gut 37 and inflammatory bowel disease3, also fl-hexosaminidase from other sources such as mucosa can cause lesions in other tissues39. We suggest that the increased spontaneous secretion in jaundice may also be harmful to patients.…”

Section: Discussionmentioning

confidence: 99%

Monocyte Secretion of β‐Hexosaminidase in Patients With Obstructive Jaundice

Jiang

Puntis

1993

HPB Surgery

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Monocyte hydrolases are harmful when secreted inappropriately. In this study we have investigated the levels of one of the hydrolases, β-hexosaminidase in patients with obstructive jaundice. These patients showed markedly elevated plasma levels, and their monocytes show increased spontaneous secretion and total enzyme content. The plasma enzyme levels correlate with monocyte enzyme content as well as bile salt, and bilirubin levels, the high levels may also reflect Kupffer cell damage, as these cells clear the enzyme. Compared with controls monocytes from jaundiced patients show reduced enzyme secretion after PMA stimulation, in vitro, and unchanged secretion after zymozan stimulation. There is a difference between plasma enzyme levels in benign and malignant patients but this does not provide a clear distinction between the two groups. We conclude that patients with obstructive jaundice have increased blood level of β-hexosaminidase, and that activated monocytes partly contribute to this change.

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Section: Discussionmentioning

confidence: 99%

Monocyte Secretion of β‐Hexosaminidase in Patients With Obstructive Jaundice

Jiang

Puntis

1993

HPB Surgery

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“…-glucuronidase and aryl sulphatase high enzyme β secretion was suggestive of continuous breakdown of aryl substituted and -glucuronidation β reactions during cytolysis [54]. No data is available on liver cell enzymes estimated in liver cells from liver biopsy of nitroimidazole treated livers.…”

Section: Role Of Enzymes In Energy Metabolic Integrity In Hypoxiamentioning

confidence: 99%

“…Liver cell enzymes may expand the better biochemical explanation of complex hypoxia state at molecular level. However, several scattered studies on serum choline esterase, alkaline phosphatase, glucose-6P-dehydrogenase, ornithine carbamoyl transferase, cyclooxygenase2, lactate dehydrogenase enzymes have been reported significant in hepatic hypoxia or hepatic damage evaluation with addition of new members of enzymes [54][55][56].…”

Section: Role Of Enzymes In Energy Metabolic Integrity In Hypoxiamentioning

confidence: 99%

The Hepatocellular Hypoxia Criteria:2’Nitroimidazole Effect on Hepatocyte Carbohydrate Metabolizing Enzymes

Sharma

2008

Nat Prec

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Aim: to understand the 2'-nitroimidazole induced hypoxia and liver cell interaction, we proposed a "Hapatocellular Hypoxia Criteria". Hypothesis: The nitroimidazole induced metabolic energy loss and oxygen depletion (hypoxia) in liver cell mitochondria causes the phagocytosis. Based on it, ten control subjects with 2'-nitroimidazole therapy were studied for their carbohydrate metabolizing enzymes in serum and hepatocellular enzymes in liver biopsy tissues. Materials and Methods: Proven ten control subjects were studied for hypoxia by enzyme assays. The 2'nitroimidazole treated paired ten subjects were studied for hypoxia using enzyme assays and hepatocellular cytomorphology by electron microscopy. Results and Discussion: Out of ten subjects on 2'-nitroimidazole, nine showed elevated carbohydrate metabolizing and lysosomal enzyme levels in serum. The enzymes glucokinase (in 80% samples), aldolase (in 80% samples), phosphofructokinase (in 80% samples), malate dehydrogenase (in 75% samples), isocitrate dehydrogenase (ICDH) (in 60% patients) were elevated while succinate dehydrogenase and lactate dehydrogenase (LDH) levels remained unaltered. Lysosomal enzymes-glucuronidase, alkaline phosphatase, acid phosphatase, β showed enhanced levels in the serum samples. In control ten liver biopsies, the hepatocytes and Kupffer cell preparations showed altered enzyme levels. Hepatocytes showed lowered glucokinase (in 80%), LDH (in 80%), and higher content of aldolase (in 80%), pyruvate kinase (in 100%), malate dehydrogenase (in 80%), ICDH (in 80%), citrate dehydrogenase (in 70%), phosphogluconate dehydrogenase (in 80%). Kupffer cells showed higher enzyme levels of-β glucuroronidase (in 80%), leucine aminopeptidase (in 70%), acid phosphatase (in 80%) and aryl sulphatase (in 88%). In these 10 biopsy samples from subjects on 2'-nitronidazole clinical trial, the electron microscopy cytomorphology observations showed swollen bizarre mitochondria, proliferative endoplasmic reticulum, and anisonucleosis after 2'-Nitroimidazole effect in liver cell damage. Conclusion: The proposed "Hepatocellular Hypoxia Criteria" served to define origin of liver hypoxia and showed altered hepatic enzyme activities with active phagocytosis and cytotoxicity in subjects after 2'-nitroimidazole treatment. The study suggests the enzyme

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Generation of reactive oxygen species by Kupffer cells and blood monocytes during intestinal amebiasis in guinea pigs

et al. 1988

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The reactive oxygen species generation capacity of Kupffer cells and blood monocytes was studied through chemiluminescence (CL) in guinea pigs, infected intracecally with Entameba histolytica, on days 0, 3, 7, 14 and 21 post infection. There was an elevated response up to the 14th post-infection day. The CL response of these cells was diminished on the 21st post infection day though it was significantly higher than up the controls. The CL response increased exponentially with the severity of cecal lesions. A direct correlation was observed between the CL response of phagocytic cells and cecal lesions of animals. The role of oxygen species in causation of tissue injury during intestinal amebiasis is postulated.

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Role of β-glucuronidase, a lysosomal enzyme, in the pathogenesis of intestinal amoebiasis: an experimental study

Cited by 4 publications

References 16 publications

Monocyte Secretion of β‐Hexosaminidase in Patients With Obstructive Jaundice

Monocyte Secretion of β‐Hexosaminidase in Patients With Obstructive Jaundice

The Hepatocellular Hypoxia Criteria:2’Nitroimidazole Effect on Hepatocyte Carbohydrate Metabolizing Enzymes

Generation of reactive oxygen species by Kupffer cells and blood monocytes during intestinal amebiasis in guinea pigs

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