Role of WUSCHEL in Regulating Stem Cell Fate in the Arabidopsis Shoot Meristem

Mayer, Klaus; Schoof, Heiko; Haecker, Achim; Lenhard, Michael; Jürgens, Gerd; Laux, Thomas

doi:10.1016/s0092-8674(00)81703-1

Cited by 1,511 publications

(1,466 citation statements)

References 54 publications

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“…WUSCHEL ( WUS ) is a homeodomain TF that is expressed in flower and shoot meristems, where it induces stem cell fate in a non‐cell‐autonomous manner (Laux, Mayer, Berger, & Jurgens, 1996; Mayer et al., 1998). Overexpression of WUS in arabidopsis is sufficient to induce organogenesis and SE in the shoot and root tip (Chatfield et al., 2013; Gallois, Nora, Mizukami, & Sablowski, 2004).…”

Section: A Network Of Arabidopsis Transcription Factors Controls Somamentioning

confidence: 99%

A transcriptional view on somatic embryogenesis

2017

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Somatic embryogenesis is a form of induced plant cell totipotency where embryos develop from somatic or vegetative cells in the absence of fertilization. Somatic embryogenesis can be induced in vitro by exposing explants to stress or growth regulator treatments. Molecular genetics studies have also shown that ectopic expression of specific embryo‐ and meristem‐expressed transcription factors or loss of certain chromatin‐modifying proteins induces spontaneous somatic embryogenesis. We begin this review with a general description of the major developmental events that define plant somatic embryogenesis and then focus on the transcriptional regulation of this process in the model plant Arabidopsis thaliana (arabidopsis). We describe the different somatic embryogenesis systems developed for arabidopsis and discuss the roles of transcription factors and chromatin modifications in this process. We describe how these somatic embryogenesis factors are interconnected and how their pathways converge at the level of hormones. Furthermore, the similarities between the developmental pathways in hormone‐ and transcription‐factor‐induced tissue culture systems are reviewed in the light of our recent findings on the somatic embryo‐inducing transcription factor BABY BOOM.

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Section: A Network Of Arabidopsis Transcription Factors Controls Somamentioning

confidence: 99%

A transcriptional view on somatic embryogenesis

2017

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“…The class I KNOX genes, which include STM, BP, KNAT2, and KNAT6, are also implicated in SAM function (Lincoln et al, 1994;Long et al, 1996;Semiarti et al, 2001). The WUSCHEL (WUS) is required for stem cell function in the SAM (Laux et al, 1996;Mayer et al, 1998). The CLA-VATA3 (CLV3) is involved in restricting stem cell population in the SAM by negatively regulating WUS (Brand et al, 2000;Schoof et al, 2000).…”

Section: Ectopic Expression In 35s::cuc1 Seedlings Of Genes Involvedmentioning

confidence: 99%

CUC1 gene activates the expression of SAM‐related genes to induce adventitious shoot formation

Hibara

Takada

Tasaka³

2003

The Plant Journal

177

138

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SummaryCUP-SHAPED COTYLEDON (CUC )1 encodes members of the NAC family. These are functionally redundant genes that are involved in shoot apical meristem (SAM) formation and cotyledon separation during embryogenesis in Arabidopsis. We analyzed transgenic plants overexpressing CUC1 (35S::CUC1 ). The cotyledons of these transgenic seedlings regularly had two basal lobes, small and round epidermal cells between the sinuses, and adventitious SAMs on the adaxial surface of this region. This suggests that CUC1 promotes adventitious SAM formation by maintaining epidermal cells in an undifferentiated state. In 35S::CUC1 cotyledons, the class I knotted-like homeobox (KNOX ) genes, including SHOOT MERISTEMLESS (STM ) and BREVIPEDICELLUS (BP ), which are involved in SAM formation and/or maintenance, were ectopically expressed before adventitious SAM formation. In stm mutants, ectopic expression of CUC1 could not induce adventitious SAMs, whereas they continued to be observed in bp mutants. These results suggest that STM, but not BP, is necessary for the formation of adventitious SAMs in 35S::CUC1 cotyledons. Furthermore, we examined the relationship between CUC1 and ASYMMETRIC LEAVES (AS )1 and AS2. The as1 and as2 mutations genetically enhance 35S::CUC1 phenotypes even in the absence of STM function. Interestingly, the as1 mutation can partially rescue the mutant vegetative development phenotypes in the cuc1 cuc2 double mutant. Our results suggest that CUC1 positively regulates SAM formation not only through STM but also through an STM-independent pathway that is negatively regulated by AS1 and AS2.

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“…The pga6 mutant was found to efficiently produce somatic embryos on seedlings and explants in the presence of the inducer. Molecular analysis demonstrated that PGA6 was identical to the homeobox gene WUSCHEL (WUS) [21], which regulates stem cell fate in shoot and floral meristems [23][24][25]. Moreover, ectopic expression of WUS also induces shoot and floral stem cell activities in roots and stems, respectively [26,27].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis

et al. 2008

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Formation of somatic embryos from non-germline cells is unique to higher plants and can be manipulated in a variety of species. Previous studies revealed that overexpression of several Arabidopsis genes, including WUSCHEL (WUS)/PLANT GROWTH ACTIVATOR6 (PGA6), BABY BOOM, LEAFY COTYLEDON1 (LEC1), and LEC2, is able to cause vegetative-to-embryonic transition or the formation of somatic embryos. Here, we report that a gain-offunction mutation in the Arabidopsis PGA37 gene, encoding the MYB118 transcription factor, induced vegetative-toembryonic transition, the formation of somatic embryos from root explants, and an elevated LEC1 expression level. Double mutant analysis showed that WUS was not required for induction of somatic embryos by PGA37/MYB118. In addition, overexpression of MYB115, a homolog of PGA37/MYB118, caused a pga37-like phenotype. A myb118 myb115 double mutant did not show apparent developmental abnormalities. Collectively, these results suggest that PGA37/ MYB118 and MYB115 promote vegetative-to-embryonic transition, through a signaling pathway independent of WUS.

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Role of WUSCHEL in Regulating Stem Cell Fate in the Arabidopsis Shoot Meristem

Cited by 1,511 publications

References 54 publications

A transcriptional view on somatic embryogenesis

A transcriptional view on somatic embryogenesis

CUC1 gene activates the expression of SAM‐related genes to induce adventitious shoot formation

Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis

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