Role of vacuolar membrane proton pumps in the acidification of protein storage vacuoles following germination

Wilson, Karl A.; Chavda, Burzin; Pierre-Louis, Gandhy; Quinn, Adam; Tan-Wilson, Anna L.

doi:10.1016/j.plaphy.2016.03.031

Cited by 8 publications

(6 citation statements)

References 39 publications

(4 reference statements)

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“…A recent report suggested that GA and S together decrease oxidative stress by ethylene formation and promoting S use efficiency, photosynthesis and GSH production of Cd stressed plants. 29 For the regulation of S assimilation BRs can be modified by sulfation and sulfotransferases, thus exert their role on S metabolism. 30 The interplay of S with nitric oxide Nitric oxide is reported to be involved in decreasing oxidative damage through GSH generation under various abiotic stresses.…”

Section: E1477905-2mentioning

confidence: 99%

Interaction of sulfur with phytohormones and signaling molecules in conferring abiotic stress tolerance to plants

Hasanuzzaman

Bhuyan

Mahmud

et al. 2018

Plant Signaling & Behavior

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Sulfur (S) is a macronutrient for the plant, which has an immense role in basic plant processes and regulation of several metabolic pathways. It has also a major role in providing protection against adverse conditions. Sulfur-containing amino acids and metabolites maintain plant cell mechanisms to improve stress tolerance. It interacts with several biomolecules such as phytohormones, polyamines, nitric oxide (NO), and even with other plant nutrients, which can produce some derivatives those are essential for abiotic stress tolerance. Different S derivatives stimulate signaling cascades, for the upregulation of different cellular messengers such as abscisic acid, Ca, and NO. Sulfur is also known to interact with some essential plant nutrients by influencing their uptake and transport, hence, confers nutrient homeostasis efficiencies. This review focuses on how S is interacted with several signaling molecules like NO, glutathiones, phytohormones, hydrogen sulfide, polyamines, etc. This is a concise summary aimed at guiding the researchers to study S-related plant processes in the light of abiotic stress tolerance.

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Section: E1477905-2mentioning

confidence: 99%

Interaction of sulfur with phytohormones and signaling molecules in conferring abiotic stress tolerance to plants

Hasanuzzaman

Bhuyan

Mahmud

et al. 2018

Plant Signaling & Behavior

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“…Then, we examined if these plants had any defects at the cellular level. During germination of wild-type seeds, one of the most prominent changes is the fusion of PSV to each other to give rise to the LV (17,18). The atcap2-1 mutant showed delayed germination compared with wild-type seeds (SI Appendix, Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Another important feature that accompanies PSV conversion to the LV is storage protein degradation (18,24). When we examined the degradation of two storage proteins at the biochemical level ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Seed germination is a complex process mediated by the conversion of seed embryonic cells to vegetative cells of the growing seedling. The PSV undergoes conversion to the LV during germination, which depends on the correct trafficking and localization of many newly synthesized proteins (3,18). These proteins include enzymes that degrade PSV proteins, proteins that mediate fusion of PSVs, and proteins that mediate LV acidification and activity.…”

Section: Discussionmentioning

confidence: 99%

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AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking

Kwon

Shen

Lee

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Protein trafficking is a fundamental mechanism of subcellular organization and contributes to organellar biogenesis. AtCAP2 is an homolog of the calcium-dependent protein kinase 1 adaptor protein 2 (McCAP2), a member of the syntaxin superfamily. Here, we show that AtCAP2 plays an important role in the conversion to the lytic vacuole (LV) during early plant development. The loss-of-function mutant displayed delays in protein storage vacuole (PSV) protein degradation, PSV fusion, LV acidification, and biosynthesis of several vacuolar proteins during germination. At the mature stage, plants accumulated vacuolar proteins in the prevacuolar compartment (PVC) instead of the LV. In wild-type plants, AtCAP2 localizes to the PVC as a peripheral membrane protein and in the PVC compartment recruits glyceraldehyde-3-phosphate dehydrogenase C2 (GAPC2) to the PVC. We propose that AtCAP2 contributes to LV biogenesis during early plant development by supporting the trafficking of specific proteins involved in the PSV-to-LV transition and LV acidification during early stages of plant development.

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“…They can be generally classified into two families: 7S and 11-12S (Shewry et al, 2002;Wilson, Chavda, Pierre-Louis, Quinn & Tan-Wilson, 2016;Zhang et al, 2016). The 7S globulins are stored in protein bodies and act only as storage proteins; the globulins in the aleurone and embryo have little impact on the properties of grains, even though they are rich in these tissues (Shewry et al, 2002).…”

Section: Storage Globulinsmentioning

confidence: 99%

Mechanism of reduction in starch digestion rate of Durum Wheat by protein

Zou¹

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The aim of the project is to explore the mechanism of durum wheat proteins in slowing starch digestion; this reduction in digestion rate is nutritionally advantageous. The grains of three commercial durum wheat varieties (Jandaroi, Caparoi and Yawa) were employed, from which a range of pasta-derived cooked substrates were prepared: semolina (SE), whole pasta (spaghetti) (WP), powdered pasta (PP) and extracted starch (ST). SE contains inherent protein components; WP has an intact compact structure and gluten network formed by kneading and extruding the SE proteins; PP was ground from WP, thus breaking up the intact compact structure while the gluten network remained intact; and ST was extracted from SE with removal of proteins. To understand how pasta compact structure and proteins influence starch digestion, all these starch-containing samples with different protein composition and structure were subjected to in vitro digestion with various combinations of treatments mimicking gastric conditions with acid and pepsin, before the starch was digested with porcine α-amylase or pancreatin. After plotting the percentage of starch digested vs. time, first-order kinetics characterization through logarithm-of-slope analysis and morphological characterization by confocal microscopy were combined to reveal how the pasta compact structure and gluten network together slow starch digestion rate. Digested samples were collected at different times to characterize the weight distributions of branched starch molecules (wbr (logR h ), R h being hydrodynamic radius) using size-exclusion chromatography (SEC, also termed GPC). These distributions, together with the measured activity of α-amylase in the digestive solution, were used to explore the role that the compact structure and proteins in pasta play in retarding the evolution of starch molecular structure during digestion. Gluten powder (GP) extracted from SE was cooked and centrifuged to separate supernatant from gluten as a pellet, followed by the addition of α-amylase, to characterize to what extent α-amylase interacted with wheat proteins by measuring the activity of α-amylase, to elucidate if the protein components are capable of reducing enzymatic II activity; the analytical technique used was high performance liquid chromatography-mass spectrometry (LC-MS).The results showed that ST and SE were digested following simple first-order kinetics, while WP and PP followed two sequential first-order steps. The rate coefficients for these various steps were altered by pepsin hydrolysis. Confocal microscopy revealed that, following cooking, starch granules were completely swollen for ST, SE and PP samples. In WP, the granules were completely swollen in the external regions, partially swollen in the intermediate region and almost intact in the WP strand center. Gluten entrapment accounts for the sequential kinetic steps in the digestion of pasta starch; the compact microstructure of pasta also reduces digestion rates. A reduced activity of porcine α-amylase and retarded dig...

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Role of vacuolar membrane proton pumps in the acidification of protein storage vacuoles following germination

Cited by 8 publications

References 39 publications

Interaction of sulfur with phytohormones and signaling molecules in conferring abiotic stress tolerance to plants

Interaction of sulfur with phytohormones and signaling molecules in conferring abiotic stress tolerance to plants

AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking

Mechanism of reduction in starch digestion rate of Durum Wheat by protein

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