Role of tumour necrosis factor in lung injury caused by intestinal ischaemia–reperfusion

Abstract

Treatment with an anti-TNF antibody resulted in a significant attenuation of lung injury following intestinal I/R. The data indicate that TNF is an important trigger for upregulation of pulmonary endothelial and neutrophil adhesion molecules after intestinal I/R.

Interleukin-10 Gene Therapy Attenuates Pulmonary Tissue Injury Caused by Mesenteric Ischemia-Reperfusion in a Mouse Model

“…Lung tissue wet/dry ratio and Evans blue dye concentration were used in the current study to assess the changes in the pulmonary microvascular permeability. In our model, Evans blue dye concentration and the lung tissue wet/dry ratio of animals sustaining mesenteric I/R were significantly higher than that of controls and, a finding consistent with previous studies (Koksoy et al 2001). The lung edema expressed by both increased Evans blue dye concentration and tissue wet/dry ratio was significantly reduced with the use of pre-ischemic IL-10 gene overexpression.…”

“…There are several methods to evaluate the degree of pulmonary edema in literature (Iglesias et al 1998;Koike et al 2000;Koksoy et al 2001;Dowdall et al 2002). Lung tissue wet/dry ratio and Evans blue dye concentration were used in the current study to assess the changes in the pulmonary microvascular permeability.…”

“…Pulmonary tissue neutrophil sequestration was determined according to the method described by Koksoy et al (2001). A single pathologist blinded to the groups examined at least two different sections of each specimen.…”

Perfil temporal da inflamação pulmonar induzida pela isquemia/reperfusão intestinal em ratos. Estudo do papel do sistema linfático.

“…Estudos experimentais demonstram o aumento dos seus níveis plasmáticos durante a I/R intestinal, sendo a lesão pulmonar aguda com infiltrado neutrofílico e aumento da permeabilidade características de inflamação pulmonar observadas em modelos de injeção intravenosa de TNF- (OKUSAWA et al, 1998). Além disso, Koksoy et al (2001) mostraram que o bloqueio do TNF- com anticorpos reduz o infiltrado de neutrófilos e o aumento da permeabilidade vascular pulmonar induzido pela I/R intestinal. No entanto, Gaines et al (1999) demonstraram que em animais submetidos a I/R da pata anterior, o tratamento com receptores solúveis para TNF- reduz a lesão microvascular, mas não o recrutamento pulmonar de neutrófilos, demonstrando o complexo papel desta citocina na lesão e inflamação pulmonar aguda pós I/R.…”

“…Diversos relatos indicam que indivíduos submetidos à I/R intestinal desenvolvem lesão pulmonar aguda (MITSUOKA et al, 1999;KOKSOY et al, 2001;KUZU et al, 2002). Nesse sentido, a despeito de seus efeitos benéficos inquestionáveis, a reperfusão tecidual subsequente aos quadros de isquemia, é importante fator de risco para o desencadeamento de lesões teciduais generalizadas graves onde o pulmão é o principal órgão afetado.…”