Role of the ubiquitin‐proteasome pathway on proteolytic activity in postmortem proteolysis and tenderisation of sheep skeletal muscle

Liu, Yue; Du, Ming; Li, Xin; Chen, Li; Shen, Qingwu; Tian, Jianwen; Zhang, Dequan

doi:10.1111/ijfs.13214

Cited by 11 publications

(7 citation statements)

References 28 publications

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“…BIRC6 is part of the ubiquitin-mediated proteolysis pathway and was positively associated with M17. By impairing proteolysis, the up-regulation of BIRC6 likely increases shear force ( Liu et al, 2016 ). Genes from the E3 ubiquitin-protein ligase family ( HERC1, HERC2, HUWE1, ITCH , and UBR5 ) were also identified in agreement with a recent report that found ubiquitination and apoptosis to be potential regulators of meat tenderness in Nelore cattle ( Gonçalves et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Detection of Co-expressed Pathway Modules Associated With Mineral Concentration and Meat Quality in Nelore Cattle

et al. 2019

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Meat quality is a complex trait that is influenced by genetic and environmental factors, which includes mineral concentration. However, the association between mineral concentration and meat quality, and the specific molecular pathways underlying this association, are not well explored. We therefore analyzed gene expression as measured with RNA-seq in Longissimus thoracis muscle of 194 Nelore steers for association with three meat quality traits (intramuscular fat, meat pH, and tenderness) and the concentration of 13 minerals (Ca, Cr, Co, Cu, Fe, K, Mg, Mn, Na, P, S, Se, and Zn). We identified seven sets of co-expressed genes (modules) associated with at least two traits, which indicates that common pathways influence these traits. From pathway analysis of module hub genes, we further found an over-representation for energy and protein metabolism (AMPK and mTOR signaling pathways) in addition to muscle growth, and protein turnover pathways. Among the identified hub genes FASN, ELOV5, and PDE3B are involved with lipid metabolism and were affected by previously identified eQTLs associated to fat deposition. The reported hub genes and over-represented pathways provide evidence of interplay among gene expression, mineral concentration, and meat quality traits. Future studies investigating the effect of different levels of mineral supplementation in the gene expression and meat quality traits could help us to elucidate the regulatory mechanism by which the genes/pathways are affected.

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Section: Discussionmentioning

confidence: 99%

Detection of Co-expressed Pathway Modules Associated With Mineral Concentration and Meat Quality in Nelore Cattle

et al. 2019

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“…The TEM examination of muscle structure was conducted as described by Liu et al . () with some modification. Muscle (4 × 2 × 2 mm) was submitted to double fixation, (2% glutaraldehyde, pH 7.4; 1.0% osmium tetroxide), dehydrated through a graded ethanol (30%, 50%, 60%, 70%, 80%, 90%, 100%), replaced three times with anhydrous acetone and then embedded in an Epon–Araldite mixture.…”

Section: Methodsmentioning

confidence: 99%

Changes in degradation and phosphorylation level of titin in three ovine muscles during postmortem

Wang

et al. 2017

Int J of Food Sci Tech

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The objective of this study was to investigate the degradation of titin and its phosphorylation level in three muscles from sheep. The MFI and pH from the longissimus lumborum (LL), semimembranosus (SM) and psoas major (PM) muscles were measured at 30 min, 1, 2, 7, 14, 21 and 28 days postmortem. Myofibrillar proteins were extracted, separated by SDS-PAGE and quantified by phosphor-specific staining. Phosphorylation of titin was predicted by Pro-Q Diamond-SYPRO Ruby staining. Two days after exsanguination, the pH and MFI of the PM were higher than those of the LL and SM muscles (P < 0.05). The sarcomere length of the PM muscle was also longer than that of the LL and SM muscle (P < 0.05). PM muscles had a highest phosphorylation level (P < 0.05) at 0.5 h postmortem and showed the greatest degree of titin degradation over 28 days. This suggests that phosphorylation of titin might accelerate its degradation.

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“…Recent studies have shown that muscle atrophy occurs due to increased proteolysis due to the activation of the ubiquitin-proteasome pathway (Jagoe and Goldberg, 2001;Bodine and Baehr, 2014;Liu et al, 2016). In particular, muscle atrophy F-box (MAFbx)/atrogin-1 and muscle RING finger-1 (MuRF-1), which are muscle-specific ubiquitin ligases, are expressed early in the muscle atrophy process and are directly involved in muscle protein degradation (Bodine and Baehr, 2014;Liu et al, 2016).…”

Section: Inhibitory Activity Of Deer Antler Extracts On Muscle Atrophy Factors In Aicar-induced Muscle Atrophied C2c12 Cellsmentioning

confidence: 99%

Effect of Deer Antler Extract on Muscle Differentiation and 5-Aminoimidazole-4-Carboxamide Ribonucleoside (AICAR)-Induced Muscle Atrophy in C2C12 Cells

Jo¹,

Jang²,

Yun³

et al. 2021

Food Sci Anim Resour

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The effect of deer antler extract on muscle differentiation and muscle atrophy were evaluated to minimize muscle loss following aging. Various deer antler extracts (HWE, hot water extract of deer antler; FE, hot water extract of fermented deer antler; ET, enzyme-assisted extract of deer antler; UE, extract prepared by ultrasonication of deer antler) were evaluated for their effect on muscle differentiation and inhibition of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR)-induced muscle atrophy in C2C12 cells. To confirm the morphological changes caused by the effect of deer antler extracts on muscle differentiation, the length and thickness of myotubes were measured by Jenner-Giemsa staining. In addition, the expression levels of genes related to muscle differentiation and atrophy were confirmed through qRT-PCR. In the presence of antler extracts, the length and thickness of myotubes and MyoD1 and Myf5 gene expression were increased compared to those in the control group. Gene expression of AMPK, MyoD1, and myogenin, along with the muscle atrophy factors MuRF-1 and FoxO3a upon addition of deer antler extracts to muscle-atrophied C2C12 cells was determined by qRT-PCR after treatment with AICAR. The expression of MuRF-1 and FoxO3a decreased in the groups treated with antler extracts compared to that in the group treated with AICAR alone. In addition, gene expression of MyoD1 and myogenin in the muscle atrophy cell model was significantly increased compared that into the control group. Therefore, our findings indicate that deer antler extract can increase the expression of MyoD1, Myf5 and myogenin, inhibit muscle atrophy, and promote muscle differentiation.

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Role of the ubiquitin‐proteasome pathway on proteolytic activity in postmortem proteolysis and tenderisation of sheep skeletal muscle

Cited by 11 publications

References 28 publications

Detection of Co-expressed Pathway Modules Associated With Mineral Concentration and Meat Quality in Nelore Cattle

Detection of Co-expressed Pathway Modules Associated With Mineral Concentration and Meat Quality in Nelore Cattle

Changes in degradation and phosphorylation level of titin in three ovine muscles during postmortem

Effect of Deer Antler Extract on Muscle Differentiation and 5-Aminoimidazole-4-Carboxamide Ribonucleoside (AICAR)-Induced Muscle Atrophy in C2C12 Cells

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