Role of the receptor for bacteriophage lambda in the functioning of the maltose chemoreceptor of Escherichia coli

Hazelbauer, Gerald L.

doi:10.1128/jb.124.1.119-126.1975

Cited by 52 publications

(22 citation statements)

References 17 publications

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“…5). These results strengthen the evidence that MBP and maltoporin are the only products of the mal regulon that contribute to maltose chemotaxis (20,21). The concentration of MBP in the reconstitution mixture that was needed to give maximal accumulation in maltosecontaining capillaries was 0.5 mM for a malT AmalB1J2 zib-729::TnlO strain (MM162) and 21 mM for a AmalE zib-729::TnlO strain (MM129; Table 5).…”

Section: Discussionsupporting

confidence: 74%

“…The sensitivity of the capillary assay is very low in strains lacking maltoporin (21). To solve this problem, we took advantage of the malT-independent lamB gene expression from ;jb-729::TnJO (see above).…”

Section: Resultsmentioning

confidence: 99%

“…To avoid the problem of small sample size, we turned to the capillary test. Since strains lacking maltoporin perform poorly in the maltose capillary assay (21), we used two methods to construct malT and malBJ12 strains that express the lamB gene: (i) we crossed the transposon zjb-729::TnJO, which supports malT-independent expression of the lamB gene, into strains by P1 transduction (see above); or (ii) we lysogenized strains with the phages XYh°434LB8 (39) and Xcu3h0434 (26), which carry the lamB gene downstream from the lacL8UV5 promoter ( Fig. 5).…”

Section: Discussionmentioning

confidence: 99%

“…Interaction of maltose-loaded MBP with Tar in vitro has been demonstrated (30,44). The product of the lamB gene, maltoporin, facilitates maltose permeation through the outer membrane (50, 51), increasing the chemotactic sensitivity of the cells to maltose by 100-fold (21). The malT gene codes for a positive activator needed for expression of malE, lamB, and all other malA and malB region genes except malT itself (19).…”

mentioning

confidence: 99%

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Reconstitution of maltose chemotaxis in Escherichia coli by addition of maltose-binding protein to calcium-treated cells of maltose regulon mutants

Brass¹,

Manson²

1984

J Bacteriol

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Maltose chemotaxis was reconstituted in AmalE cells lacking maltose-binding protein (MBP). Purified MBP was introduced into intact cells during incubation with 250 mM CaCl2 in Tris-hydrochloride buffer at 0C. After removal of extracellular CaCl2 and MBP, chemotaxis was measured with tethered bacteria in a flow chamber or with free-swimming cells in a capillary assay. About 20% of tethered cells responded to 10-4 M maltose; the mean response times were about half those of CaCl2-treated wild-type cells (100 s as opposed to 190 s). In capillary tests, the maltose response of reconstituted cells was between 15 and 40% of the aspartate response, about the same percentage as in wild-type cells. The best reconstitution was seen with 0.5 to 1 mM MBP in the reconstitution mixture, which is similar to the periplasmic MBP concentration estimated for maltose-induced wild-type cells. Strains containing large deletions of the malB region and malT mutants lacking the positive regulator gene of the mal regulon also could be reconstituted for maltose chemotaxis, showing that no product of the mal regulon other than MBP is essential for maltose chemotaxis.1. Adler, J. 1973. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli.

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Section: Discussionsupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Reconstitution of maltose chemotaxis in Escherichia coli by addition of maltose-binding protein to calcium-treated cells of maltose regulon mutants

Brass¹,

Manson²

1984

J Bacteriol

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“…Phages have been instrumental in the detection of dualfunction surface receptors on a variety of microbes (3,6,9,16,20,22,25). For instance, two recently isolated classes of phage-resistant mutants of Actinomyces viscosus MG-1 were altered simultaneously in their interbacterial adherence to certain strains of Streptococcus sanguis, indicating that the phage receptor and one of the adhesins on A. viscosus MG-1 are related in an as-yet-unspecified manner (24).…”

mentioning

confidence: 99%

Use of lytic bacteriophage for Actinomyces viscosus T14V as a probe for cell surface components mediating intergeneric coaggregation

et al. 1988

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A lytic bacteriophage for Actinomyces viscosus T14V (the reference strain for actinomyces coaggregation group A) was isolated from raw sewage. This phage, designated BF307, also lysed the T14V-derived nonfimbriated mutant PK455-2 as well as A. viscosus MG-1 and T14AV but not the other serotype 2 or serotype 1 strains of this species that were tested or any of nine Actinomyces naeslundii isolates. Phages BF307 belonged to Bradley morphological group C and was similar in appearance to the A. viscosus MG-1 phages Av-1 and Av-3, which do not productively infect A. viscosus T14V. A. viscosus MG-1 mutants selected for resistance to phage BF307, Av-3, or CT7 (a human dental plaque isolate with the same host range as BF307) were coresistant to the other two phages but sensitive to Av-1. These results indicate that the receptors on A. viscosus MG-1 for phages BF307, Av-3, and CT7 are identical or share a common precursor and that the receptor for phage Av-1 is distinct. Comparison of the genomes of BF307, Av-3, and CT7 revealed that their DNAs were similar in size but distinguishable by restriction analysis. Two altered coaggregation phenotypes were identified among the phage BF307-resistant mutants of strains MG-1, T14V, T14AV, and PK455-2. Class I mutants had lost the ability to interact with coaggregation group 1 streptococci, and class II mutants did not coaggregate with either group 1 or group 2 streptococci. These results are consistent with the proposal that the phage BF307 receptor on these A. viscosus strains is related to one of the structures that mediates coaggregation with oral streptococci. A model to delineate the various coaggregation mediators on the surface of actinomyces coaggregation group A cells is presented, and the use of these phages to probe surface components of human oral actinomyces strains is discussed.

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Viral Receptors Serving Host Functions

Co¹,

Fields²,

Greene³

1986

Concepts in Viral Pathogenesis II

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Role of the receptor for bacteriophage lambda in the functioning of the maltose chemoreceptor of Escherichia coli

Cited by 52 publications

References 17 publications

Reconstitution of maltose chemotaxis in Escherichia coli by addition of maltose-binding protein to calcium-treated cells of maltose regulon mutants

Reconstitution of maltose chemotaxis in Escherichia coli by addition of maltose-binding protein to calcium-treated cells of maltose regulon mutants

Use of lytic bacteriophage for Actinomyces viscosus T14V as a probe for cell surface components mediating intergeneric coaggregation

Viral Receptors Serving Host Functions

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