Role of the proteome in providing phenotypic stability in control and ectomycorrhizal poplar plants exposed to chronic mild Pb stress

Szuba, Agnieszka; Marczak, Łukasz; Kozłowski, Rafał

doi:10.1016/j.envpol.2020.114585

Cited by 16 publications

(7 citation statements)

References 59 publications

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“…In the present experiment, decreased protein turnover was rather related to the low Pb dose, and most importantly to the chronic nature of Pb exposure. To reduce energy-consuming processes such as protein biosynthesis [ 3 , 47 , 53 ] only proteins necessary to provide metabolome stability are upregulated. Decreased or unchanged protein turnover was previously observed in plants exposed to prolonged stress [ 49 , 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the decreased Pb uptake observed in bigger M-Pb plants coexisted with the intensive activation of molecular processes involved in Pb sequestration [ 83 ] or the redirection of the root metabolic flux into an increase in the production of Pb chelates such as citrate [ 44 ]. In this context, in inoculated but minimally colonized roots, the plant cell molecular response was more intense than that in noninoculated plants (and the opposite of that in massively colonized plants, where the fungal biofilter resulted in reduced proteome responses in plant host cells exposed to Pb; [ 53 ]) but more efficient than that in noncolonized plants. Inoculation with ectomycorrhizal fungi increases the host plant’s heavy metal tolerance (mainly via activation of molecular pathways associated with reducing Pb bioavailability/toxicity) regardless of low root colonization ratio.…”

Section: Discussionmentioning

confidence: 99%

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Pb Stress and Ectomycorrhizas: Strong Protective Proteomic Responses in Poplar Roots Inoculated with Paxillus involutus Isolate and Characterized by Low Root Colonization Intensity

Szuba

Marczak

Kozłowski

2021

IJMS

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The commonly observed increased heavy metal tolerance of ectomycorrhized plants is usually linked with the protective role of the fungal hyphae covering colonized plant root tips. However, the molecular tolerance mechanisms in heavy metal stressed low-colonized ectormyocrrhizal plants characterized by an ectomycorrhiza-triggered increases in growth are unknown. Here, we examined Populus × canescens microcuttings inoculated with the Paxillus involutus isolate, which triggered an increase in poplar growth despite successful colonization of only 1.9% ± 0.8 of root tips. The analyzed plants, lacking a mantle—a protective fungal biofilter—were grown for 6 weeks in agar medium enriched with 0.75 mM Pb(NO3)2. In minimally colonized ‘bare’ roots, the proteome response to Pb was similar to that in noninoculated plants (e.g., higher abundances of PM- and V-type H+ ATPases and lower abundance of ribosomal proteins). However, the more intensive activation of molecular processes leading to Pb sequestration or redirection of the root metabolic flux into amino acid and Pb chelate (phenolics and citrate) biosynthesis coexisted with lower Pb uptake compared to that in controls. The molecular Pb response of inoculated roots was more intense and effective than that of noninoculated roots in poplars.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pb Stress and Ectomycorrhizas: Strong Protective Proteomic Responses in Poplar Roots Inoculated with Paxillus involutus Isolate and Characterized by Low Root Colonization Intensity

Szuba

Marczak

Kozłowski

2021

IJMS

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“…6A and B). Although the analysed P. involutus hyphae may be freely penetrated by the fluorochromes used here (Szuba et al ., 2020), the low fluorescence observed in the mycorrhizal root tips may be partly due to the physical barrier created by the fungal mantle, which reduced the concentration of the fluorochromes that reached the plant cells (only in the fungus‐covered root fraction; see also Supporting Information Fig. S1).…”

Section: Resultsmentioning

confidence: 99%

Integrated proteomic and metabolomic analyses revealed molecular adjustments in Populus × canescens colonized with the ectomycorrhizal fungus Paxillus involutus, which limited plant host growth

Szuba

Marczak

Ratajczak

et al. 2020

Environmental Microbiology

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Ectomycorrhizae (ECMs) are a highly context-dependent interactions that are not always beneficial for the plant host, sometimes leading to a decrease in plant growth. However, the molecular status of these plants remains unknown. We studied Populus × canescens microcuttings characterized by impaired growth in response to colonization by a Paxillus involutus strain via integrative proteomics-metabolomics analyses. The analysed strain was characterized by low compatibility and formed only mantles, not a Hartig net, in the majority of root tips. The increased abundance of photosynthetic proteins and foliar carbohydrates co-occurred with signals of intensified resource exchange via the stems of colonized plants. In the roots, intensified C metabolism resulted in the biosynthesis of secondary C compounds unavailable to the fungal partner but also C skeletons necessary to increase insufficient N uptake from the hyphae. The stress response was also detected in colonized plants but was similar to that reported previously during mutualistic ECM interactions. In colonized poplar plants, mechanisms to prevent imbalanced C/N tradeoffs were activated. Root metabolism strongly depended on features of the whole plant, especially the foliar C/N budget. However, despite ECM-triggered growth impairment and the foliar nutrient status, the fungal partner was recognized to be a symbiotic partner.

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“…Proteomics, as a state-of-the-art technology, links these networks to protein products and addresses the biological function of proteins under various environmental stresses in crops [ 27 ]. Two-dimensional electrophoresis (2-DE) has been massively employed in the past two decades for studying protein abundance and its functional characterization on plants’ responses to heavy metal stresses, including Cd, Hg, Pb, and As [ 28 , 29 , 30 , 31 , 32 ]. However, several studies related to Cu stress have mostly been executed in Triticum aestivum L., Oenothera glazioviana , Elsholtzia splendens , Phragmites australis , and Sorghum bicolor L. [ 4 , 33 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Proteome Changes Reveal the Protective Roles of Exogenous Citric Acid in Alleviating Cu Toxicity in Brassica napus L.

Roy

Choudhury

et al. 2021

IJMS

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Citric acid (CA), as an organic chelator, plays a vital role in alleviating copper (Cu) stress-mediated oxidative damage, wherein a number of molecular mechanisms alter in plants. However, it remains largely unknown how CA regulates differentially abundant proteins (DAPs) in response to Cu stress in Brassica napus L. In the present study, we aimed to investigate the proteome changes in the leaves of B. L. seedlings in response to CA-mediated alleviation of Cu stress. Exposure of 21-day-old seedlings to Cu (25 and 50 μM) and CA (1.0 mM) for 7 days exhibited a dramatic inhibition of overall growth and considerable increase in the enzymatic activities (POD, SOD, CAT). Using a label-free proteome approach, a total of 6345 proteins were identified in differentially treated leaves, from which 426 proteins were differentially expressed among the treatment groups. Gene ontology (GO) and KEGG pathways analysis revealed that most of the differential abundance proteins were found to be involved in energy and carbohydrate metabolism, photosynthesis, protein metabolism, stress and defense, metal detoxification, and cell wall reorganization. Our results suggest that the downregulation of chlorophyll biosynthetic proteins involved in photosynthesis were consistent with reduced chlorophyll content. The increased abundance of proteins involved in stress and defense indicates that these DAPs might provide significant insights into the adaptation of Brassica seedlings to Cu stress. The abundances of key proteins were further verified by monitoring the mRNA expression level of the respective transcripts. Taken together, these findings provide a potential molecular mechanism towards Cu stress tolerance and open a new route in accelerating the phytoextraction of Cu through exogenous application of CA in B. napus.

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Role of the proteome in providing phenotypic stability in control and ectomycorrhizal poplar plants exposed to chronic mild Pb stress

Cited by 16 publications

References 59 publications

Pb Stress and Ectomycorrhizas: Strong Protective Proteomic Responses in Poplar Roots Inoculated with Paxillus involutus Isolate and Characterized by Low Root Colonization Intensity

Pb Stress and Ectomycorrhizas: Strong Protective Proteomic Responses in Poplar Roots Inoculated with Paxillus involutus Isolate and Characterized by Low Root Colonization Intensity

Integrated proteomic and metabolomic analyses revealed molecular adjustments in Populus × canescens colonized with the ectomycorrhizal fungus Paxillus involutus, which limited plant host growth

Proteome Changes Reveal the Protective Roles of Exogenous Citric Acid in Alleviating Cu Toxicity in Brassica napus L.

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